Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts

Abstract We analyze the evolutionary relationships and expression patterns of the large set of genes for chemosensory proteins (CSPs) in the two main pest locusts. We used the available transcriptome and genome data to infer the number of genes using BLAST searches and sequence similarity matrices....

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Autores principales:	R. Martín-Blázquez, B. Chen, L. Kang, M. Bakkali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93302d335ee745bcaeb5539ba2348cdd
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spelling	oai:doaj.org-article:93302d335ee745bcaeb5539ba2348cdd2021-12-02T16:07:57ZEvolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts10.1038/s41598-017-07068-02045-2322https://doaj.org/article/93302d335ee745bcaeb5539ba2348cdd2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07068-0https://doaj.org/toc/2045-2322Abstract We analyze the evolutionary relationships and expression patterns of the large set of genes for chemosensory proteins (CSPs) in the two main pest locusts. We used the available transcriptome and genome data to infer the number of genes using BLAST searches and sequence similarity matrices. Maximum likelihood phylogenies revealed the relationships between these CSPs and CSPs from several arthropods. RNAseq and qPCR allowed associating CSPs to locust phases. Crossing the phylogenetic and expression data allowed us to deduce homologies and conservation of the involvement in the phase change. We confirm that Locusta migratoria has at least 58 CSP gene copies, only five of which lack evidence of expression, and we reveal that Schistocerca gregaria has at least 42 expressed CSP genes. Both species share 21 orthologs, whereas 33 L. migratoria and 15 S. gregaria CSPs seem species-specific. Additional six S. gregaria and four L. migratoria CSPs seem duplications. Although the expression profiles are not especially conserved, seven orthologous CSP pairs share a gregarious over-expression pattern in adult locusts. We thus confirm that the number of locusts’ CSPs is large, due to gene duplications during the evolution of Orthoptera, we establish sequence and potential functional homologies, and we highlight specific CSPs that appear to be involved in locust gregariousness either in general or in a species-specific manner.R. Martín-BlázquezB. ChenL. KangM. BakkaliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. Martín-Blázquez B. Chen L. Kang M. Bakkali Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
description	Abstract We analyze the evolutionary relationships and expression patterns of the large set of genes for chemosensory proteins (CSPs) in the two main pest locusts. We used the available transcriptome and genome data to infer the number of genes using BLAST searches and sequence similarity matrices. Maximum likelihood phylogenies revealed the relationships between these CSPs and CSPs from several arthropods. RNAseq and qPCR allowed associating CSPs to locust phases. Crossing the phylogenetic and expression data allowed us to deduce homologies and conservation of the involvement in the phase change. We confirm that Locusta migratoria has at least 58 CSP gene copies, only five of which lack evidence of expression, and we reveal that Schistocerca gregaria has at least 42 expressed CSP genes. Both species share 21 orthologs, whereas 33 L. migratoria and 15 S. gregaria CSPs seem species-specific. Additional six S. gregaria and four L. migratoria CSPs seem duplications. Although the expression profiles are not especially conserved, seven orthologous CSP pairs share a gregarious over-expression pattern in adult locusts. We thus confirm that the number of locusts’ CSPs is large, due to gene duplications during the evolution of Orthoptera, we establish sequence and potential functional homologies, and we highlight specific CSPs that appear to be involved in locust gregariousness either in general or in a species-specific manner.
format	article
author	R. Martín-Blázquez B. Chen L. Kang M. Bakkali
author_facet	R. Martín-Blázquez B. Chen L. Kang M. Bakkali
author_sort	R. Martín-Blázquez
title	Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
title_short	Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
title_full	Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
title_fullStr	Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
title_full_unstemmed	Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
title_sort	evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/93302d335ee745bcaeb5539ba2348cdd
work_keys_str_mv	AT rmartinblazquez evolutionexpressionandassociationofthechemosensoryproteingeneswiththeoutbreakphaseofthetwomainpestlocusts AT bchen evolutionexpressionandassociationofthechemosensoryproteingeneswiththeoutbreakphaseofthetwomainpestlocusts AT lkang evolutionexpressionandassociationofthechemosensoryproteingeneswiththeoutbreakphaseofthetwomainpestlocusts AT mbakkali evolutionexpressionandassociationofthechemosensoryproteingeneswiththeoutbreakphaseofthetwomainpestlocusts
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Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts

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