Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea
Abstract In Korea, subtype B is the predominant variant of HIV-1, but full genome sequencing and analysis of its viral variants are lacking. We performed near full-length genome (NFLG) sequencing and phylogenetic and recombination analyses of fifty plasma samples from HIV-positive men who have sex w...
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2021
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oai:doaj.org-article:9332799b8a074a4bad57a7e4ce7f0f7e2021-12-02T12:11:40ZCharacterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea10.1038/s41598-021-82872-32045-2322https://doaj.org/article/9332799b8a074a4bad57a7e4ce7f0f7e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82872-3https://doaj.org/toc/2045-2322Abstract In Korea, subtype B is the predominant variant of HIV-1, but full genome sequencing and analysis of its viral variants are lacking. We performed near full-length genome (NFLG) sequencing and phylogenetic and recombination analyses of fifty plasma samples from HIV-positive men who have sex with men (MSM) from a Korea HIV/AIDS cohort study. Viral genomes were amplified and the near-full-length sequences were determined using next-generation sequencing (NGS) and Sanger sequencing. We focused on the HIV-1 subtype classification and identification of HIV recombinants. Twelve HIV-1 NFLGs were determined: ten were subtyped as pure HIV-1 subtype B and two recombinant strains as a common subtype CRF07_BC, and a novel subtype CRF43_02G recombined with CRF02_AG again, or a new CRF02_AG and subtype G recombinant. For the ten NFLGs determined by NGS, “the novel recombinant emerged at approximately 2003 and the other nine subtype B about 2004 or 2005”. This is the first report analyzing HIV-1 NFLG, including recombinants and clinical characteristics, by subtype among MSM in Korea. Our results provide novel insights for understanding the recombinants in the HIV-1 epidemic in Korea.Sangmi RyouMyeongsu YooKisoon KimSangsoo KimSang Il KimYoun Jeong KimDae Won ParkJun Yong ChoiHyo Youl KimJung Ho KimJoon Young SongShin-Woo KimHyun-Ha ChangBo Youl ChoiMee-Kyung KeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Sangmi Ryou Myeongsu Yoo Kisoon Kim Sangsoo Kim Sang Il Kim Youn Jeong Kim Dae Won Park Jun Yong Choi Hyo Youl Kim Jung Ho Kim Joon Young Song Shin-Woo Kim Hyun-Ha Chang Bo Youl Choi Mee-Kyung Kee Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| description |
Abstract In Korea, subtype B is the predominant variant of HIV-1, but full genome sequencing and analysis of its viral variants are lacking. We performed near full-length genome (NFLG) sequencing and phylogenetic and recombination analyses of fifty plasma samples from HIV-positive men who have sex with men (MSM) from a Korea HIV/AIDS cohort study. Viral genomes were amplified and the near-full-length sequences were determined using next-generation sequencing (NGS) and Sanger sequencing. We focused on the HIV-1 subtype classification and identification of HIV recombinants. Twelve HIV-1 NFLGs were determined: ten were subtyped as pure HIV-1 subtype B and two recombinant strains as a common subtype CRF07_BC, and a novel subtype CRF43_02G recombined with CRF02_AG again, or a new CRF02_AG and subtype G recombinant. For the ten NFLGs determined by NGS, “the novel recombinant emerged at approximately 2003 and the other nine subtype B about 2004 or 2005”. This is the first report analyzing HIV-1 NFLG, including recombinants and clinical characteristics, by subtype among MSM in Korea. Our results provide novel insights for understanding the recombinants in the HIV-1 epidemic in Korea. |
| format |
article |
| author |
Sangmi Ryou Myeongsu Yoo Kisoon Kim Sangsoo Kim Sang Il Kim Youn Jeong Kim Dae Won Park Jun Yong Choi Hyo Youl Kim Jung Ho Kim Joon Young Song Shin-Woo Kim Hyun-Ha Chang Bo Youl Choi Mee-Kyung Kee |
| author_facet |
Sangmi Ryou Myeongsu Yoo Kisoon Kim Sangsoo Kim Sang Il Kim Youn Jeong Kim Dae Won Park Jun Yong Choi Hyo Youl Kim Jung Ho Kim Joon Young Song Shin-Woo Kim Hyun-Ha Chang Bo Youl Choi Mee-Kyung Kee |
| author_sort |
Sangmi Ryou |
| title |
Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| title_short |
Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| title_full |
Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| title_fullStr |
Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| title_full_unstemmed |
Characterization of HIV-1 recombinant and subtype B near full-length genome among men who have sex with men in South Korea |
| title_sort |
characterization of hiv-1 recombinant and subtype b near full-length genome among men who have sex with men in south korea |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/9332799b8a074a4bad57a7e4ce7f0f7e |
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