Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

Exopolysaccharide (EPS) was produced by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir grains, and the preliminary structure of two EPS fractions (EPS1 and EPS2) was investigated. NMR analysis revealed that the backbone of higher producing EPS1 was consisted of →6)-α-D-Manp-(1→, →2,6)...

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Autores principales:	Luyao Xiao, Danling Xu, Nanyu Tang, Xin Rui, Qiuqin Zhang, Xiaohong Chen, Mingsheng Dong, Wei Li
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Lacticaseibacillus paracasei ZY-1 Exopolysaccharide (EPS) Structural characterization eps gene cluster Biosynthesis Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/93350834b934484c9d39906a783249ff
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spelling	oai:doaj.org-article:93350834b934484c9d39906a783249ff2021-11-26T04:41:13ZBiosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir2666-566210.1016/j.fochms.2021.100054https://doaj.org/article/93350834b934484c9d39906a783249ff2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666566221000459https://doaj.org/toc/2666-5662Exopolysaccharide (EPS) was produced by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir grains, and the preliminary structure of two EPS fractions (EPS1 and EPS2) was investigated. NMR analysis revealed that the backbone of higher producing EPS1 was consisted of →6)-α-D-Manp-(1→, →2,6)-α-D-Glcp-(1→, α-D-Manp-(1→, →2)-α-D-Glcp-(1→, →3)-α-D-Manp-(1→, →6)-α-D-Glcp-(1→. Furthermore, an eps gene cluster that encodes the glycosyltransferase and relevant proteins for EPS biosynthesis was identified on the basis of bioinformation analysis of the complete genome. RT-qPCR results indicated that wzd (ZY-1_2260) and wze (ZY-1_2259) might be essential genes involved in EPS production. Meanwhile, the synthetic mechanism of EPS1 in L. paracasei ZY-1 was further proposed. Besides, the crude and purified EPS showed certain scavenging activities against DPPH, hydroxyl and ABTS radicals. Results provided a better understanding of EPS biosynthesis in L. paracasei ZY-1 at the gene level.Luyao XiaoDanling XuNanyu TangXin RuiQiuqin ZhangXiaohong ChenMingsheng DongWei LiElsevierarticleLacticaseibacillus paracasei ZY-1Exopolysaccharide (EPS)Structural characterizationeps gene clusterBiosynthesisNutrition. Foods and food supplyTX341-641ENFood Chemistry: Molecular Sciences, Vol 3, Iss , Pp 100054- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lacticaseibacillus paracasei ZY-1 Exopolysaccharide (EPS) Structural characterization eps gene cluster Biosynthesis Nutrition. Foods and food supply TX341-641
spellingShingle	Lacticaseibacillus paracasei ZY-1 Exopolysaccharide (EPS) Structural characterization eps gene cluster Biosynthesis Nutrition. Foods and food supply TX341-641 Luyao Xiao Danling Xu Nanyu Tang Xin Rui Qiuqin Zhang Xiaohong Chen Mingsheng Dong Wei Li Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
description	Exopolysaccharide (EPS) was produced by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir grains, and the preliminary structure of two EPS fractions (EPS1 and EPS2) was investigated. NMR analysis revealed that the backbone of higher producing EPS1 was consisted of →6)-α-D-Manp-(1→, →2,6)-α-D-Glcp-(1→, α-D-Manp-(1→, →2)-α-D-Glcp-(1→, →3)-α-D-Manp-(1→, →6)-α-D-Glcp-(1→. Furthermore, an eps gene cluster that encodes the glycosyltransferase and relevant proteins for EPS biosynthesis was identified on the basis of bioinformation analysis of the complete genome. RT-qPCR results indicated that wzd (ZY-1_2260) and wze (ZY-1_2259) might be essential genes involved in EPS production. Meanwhile, the synthetic mechanism of EPS1 in L. paracasei ZY-1 was further proposed. Besides, the crude and purified EPS showed certain scavenging activities against DPPH, hydroxyl and ABTS radicals. Results provided a better understanding of EPS biosynthesis in L. paracasei ZY-1 at the gene level.
format	article
author	Luyao Xiao Danling Xu Nanyu Tang Xin Rui Qiuqin Zhang Xiaohong Chen Mingsheng Dong Wei Li
author_facet	Luyao Xiao Danling Xu Nanyu Tang Xin Rui Qiuqin Zhang Xiaohong Chen Mingsheng Dong Wei Li
author_sort	Luyao Xiao
title	Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
title_short	Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
title_full	Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
title_fullStr	Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
title_full_unstemmed	Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir
title_sort	biosynthesis of exopolysaccharide and structural characterization by lacticaseibacillus paracasei zy-1 isolated from tibetan kefir
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/93350834b934484c9d39906a783249ff
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Biosynthesis of exopolysaccharide and structural characterization by Lacticaseibacillus paracasei ZY-1 isolated from Tibetan kefir

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