Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes

Context: Silk peptide from cocoons of silkworm (Bombyx mori L., Bombycidae) has been employed as a biomedical material and exhibits various bioactivities, including immune-modulating activity. Objective: We analyzed whether silk peptide exerts direct modulating effects on NK cells using an NK cell l...

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Autores principales:	Sun-Hee Jang, Mi-Sun Oh, Hyang-Im Baek, Ki-Chan Ha, Jeong-Yong Lee, Yong-Suk Jang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	cytolytic activity complementary therapy ex vivo target cell lysis Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/933c8a43f7764eaca049c5ff54215679
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spelling	oai:doaj.org-article:933c8a43f7764eaca049c5ff542156792021-11-17T14:21:56ZSilk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes1388-02091744-511610.1080/13880209.2019.1617749https://doaj.org/article/933c8a43f7764eaca049c5ff542156792019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1617749https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Silk peptide from cocoons of silkworm (Bombyx mori L., Bombycidae) has been employed as a biomedical material and exhibits various bioactivities, including immune-modulating activity. Objective: We analyzed whether silk peptide exerts direct modulating effects on NK cells using an NK cell line in vitro and ex vivo splenocytes. We also attempted to delineate the mechanism underlying the modulation. Material and methods: In vitro activity of silk peptide on NK cells was determined by measurement of cytolytic activity against K562 cells at an effector-to-target ratio of 5:1 after incubation of NK-92MI cells with silk peptide (0–2000 μg/mL) for 48 and 72 h. Ex vivo activity of silk peptide on mouse splenic NK cells was determined similarly by using YAC-1 cells. Results: Treatment of NK-92MI NK cells with silk peptide (500–2000 μg/mL) significantly increased cytolytic activity on target cells by 2- to 4-fold. The same concentrations (500–2000 μg/mL) of silk peptide treatment also significantly enhanced the cytolytic activity of splenic NK cells against YAC-1 cells. Silk peptide treatment of IL-2-stimulated splenocytes induced enhanced expression of Th1, 2 and 17 cytokines including TNF-α, IFN-γ, IL-6, IL-4 and IL-17. Finally, ex vivo treatment with silk peptide on mouse splenocytes significantly enhanced the degree of NK cell maturation in a dose-dependent manner from 3.49 to 23.79%. Discussion and conclusions: These findings suggest that silk peptide stimulates NK cells, thereby influencing systemic immune functions and improving natural immunity. Thus, silk peptide could be useful as a complementary therapy in cancer patients.Sun-Hee JangMi-Sun OhHyang-Im BaekKi-Chan HaJeong-Yong LeeYong-Suk JangTaylor & Francis Grouparticlecytolytic activitycomplementary therapyex vivotarget cell lysisTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 369-379 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cytolytic activity complementary therapy ex vivo target cell lysis Therapeutics. Pharmacology RM1-950
spellingShingle	cytolytic activity complementary therapy ex vivo target cell lysis Therapeutics. Pharmacology RM1-950 Sun-Hee Jang Mi-Sun Oh Hyang-Im Baek Ki-Chan Ha Jeong-Yong Lee Yong-Suk Jang Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
description	Context: Silk peptide from cocoons of silkworm (Bombyx mori L., Bombycidae) has been employed as a biomedical material and exhibits various bioactivities, including immune-modulating activity. Objective: We analyzed whether silk peptide exerts direct modulating effects on NK cells using an NK cell line in vitro and ex vivo splenocytes. We also attempted to delineate the mechanism underlying the modulation. Material and methods: In vitro activity of silk peptide on NK cells was determined by measurement of cytolytic activity against K562 cells at an effector-to-target ratio of 5:1 after incubation of NK-92MI cells with silk peptide (0–2000 μg/mL) for 48 and 72 h. Ex vivo activity of silk peptide on mouse splenic NK cells was determined similarly by using YAC-1 cells. Results: Treatment of NK-92MI NK cells with silk peptide (500–2000 μg/mL) significantly increased cytolytic activity on target cells by 2- to 4-fold. The same concentrations (500–2000 μg/mL) of silk peptide treatment also significantly enhanced the cytolytic activity of splenic NK cells against YAC-1 cells. Silk peptide treatment of IL-2-stimulated splenocytes induced enhanced expression of Th1, 2 and 17 cytokines including TNF-α, IFN-γ, IL-6, IL-4 and IL-17. Finally, ex vivo treatment with silk peptide on mouse splenocytes significantly enhanced the degree of NK cell maturation in a dose-dependent manner from 3.49 to 23.79%. Discussion and conclusions: These findings suggest that silk peptide stimulates NK cells, thereby influencing systemic immune functions and improving natural immunity. Thus, silk peptide could be useful as a complementary therapy in cancer patients.
format	article
author	Sun-Hee Jang Mi-Sun Oh Hyang-Im Baek Ki-Chan Ha Jeong-Yong Lee Yong-Suk Jang
author_facet	Sun-Hee Jang Mi-Sun Oh Hyang-Im Baek Ki-Chan Ha Jeong-Yong Lee Yong-Suk Jang
author_sort	Sun-Hee Jang
title	Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
title_short	Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
title_full	Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
title_fullStr	Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
title_full_unstemmed	Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
title_sort	silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/933c8a43f7764eaca049c5ff54215679
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Silk peptide treatment potentiates natural killer cell activity in vitro and induces natural killer cell maturation and activation in mouse splenocytes

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