Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2</su...

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Autores principales:	Sana Rauf, Hafiz Imran Ahmad Qazi, Jingting Luo, Chen Fu, Ran Tao, Sajid Rauf, Lei Yang, Honglang Li, Yongqing Fu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	leaky surface acoustic wave (LSAW) immunosensor AFP MoS<sub>2</sub>@Cu<sub>2</sub>O-Au gold staining Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/933cc7f47d414ebc858ba51b9d151be1
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spelling	oai:doaj.org-article:933cc7f47d414ebc858ba51b9d151be12021-11-25T17:13:35ZUltrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids10.3390/chemosensors91103112227-9040https://doaj.org/article/933cc7f47d414ebc858ba51b9d151be12021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9040/9/11/311https://doaj.org/toc/2227-9040We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS<sub>2</sub>@Cu<sub>2</sub>O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS<sub>2</sub>@Cu<sub>2</sub>O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab<sub>2</sub>) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.Sana RaufHafiz Imran Ahmad QaziJingting LuoChen FuRan TaoSajid RaufLei YangHonglang LiYongqing FuMDPI AGarticleleaky surface acoustic wave (LSAW)immunosensorAFPMoS<sub>2</sub>@Cu<sub>2</sub>O-Augold stainingBiochemistryQD415-436ENChemosensors, Vol 9, Iss 311, p 311 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leaky surface acoustic wave (LSAW) immunosensor AFP MoS<sub>2</sub>@Cu<sub>2</sub>O-Au gold staining Biochemistry QD415-436
spellingShingle	leaky surface acoustic wave (LSAW) immunosensor AFP MoS<sub>2</sub>@Cu<sub>2</sub>O-Au gold staining Biochemistry QD415-436 Sana Rauf Hafiz Imran Ahmad Qazi Jingting Luo Chen Fu Ran Tao Sajid Rauf Lei Yang Honglang Li Yongqing Fu Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
description	We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS<sub>2</sub>@Cu<sub>2</sub>O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS<sub>2</sub>@Cu<sub>2</sub>O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab<sub>2</sub>) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.
format	article
author	Sana Rauf Hafiz Imran Ahmad Qazi Jingting Luo Chen Fu Ran Tao Sajid Rauf Lei Yang Honglang Li Yongqing Fu
author_facet	Sana Rauf Hafiz Imran Ahmad Qazi Jingting Luo Chen Fu Ran Tao Sajid Rauf Lei Yang Honglang Li Yongqing Fu
author_sort	Sana Rauf
title	Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
title_short	Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
title_full	Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
title_fullStr	Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
title_full_unstemmed	Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids
title_sort	ultrasensitive leaky surface acoustic wave immunosensor for real-time detection of alpha-fetoprotein in biological fluids
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/933cc7f47d414ebc858ba51b9d151be1
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Ultrasensitive Leaky Surface Acoustic Wave Immunosensor for Real-Time Detection of Alpha-Fetoprotein in Biological Fluids

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