Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex
The medial prefrontal cortex (mPFC), a key part of the brain networks that are closely related to the regulation of behavior, acts as a key regulator in emotion, social cognition, and decision making. Astrocytes are the majority cell type of glial cells, which play a significant role in a number of...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Frontiers Media S.A.
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9341dd5066144fe59e6c2119a90df68f |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9341dd5066144fe59e6c2119a90df68f |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9341dd5066144fe59e6c2119a90df68f2021-11-03T17:02:48ZProteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex1662-509910.3389/fnmol.2021.729975https://doaj.org/article/9341dd5066144fe59e6c2119a90df68f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnmol.2021.729975/fullhttps://doaj.org/toc/1662-5099The medial prefrontal cortex (mPFC), a key part of the brain networks that are closely related to the regulation of behavior, acts as a key regulator in emotion, social cognition, and decision making. Astrocytes are the majority cell type of glial cells, which play a significant role in a number of processes and establish a suitable environment for the functioning of neurons, including the brain energy metabolism. Astrocyte’s dysfunction in the mPFC has been implicated in various neuropsychiatric disorders. Glucose is a major energy source in the brain. In glucose metabolism, part of glucose is used to convert UDP-GlcNAc as a donor molecule for O-GlcNAcylation, which is controlled by a group of enzymes, O-GlcNAc transferase enzyme (OGT), and O-GlcNAcase (OGA). However, the role of O-GlcNAcylation in astrocytes is almost completely unknown. Our research showed that astrocytic OGT could influence the expression of proteins in the mPFC. Most of these altered proteins participate in metabolic processes, transferase activity, and biosynthetic processes. GFAP, an astrocyte maker, was increased after OGT deletion. These results provide a framework for further study on the role of astrocytic OGT/O-GlcNAcylation in the mPFC.Jun FanJun FanJun FanJun FanQiu-Ling ZhongQiu-Ling ZhongQiu-Ling ZhongQiu-Ling ZhongRan MoRan MoRan MoRan MoCheng-Lin LuCheng-Lin LuCheng-Lin LuCheng-Lin LuJing RenJing RenJing RenJing RenJia-Wen MoJia-Wen MoJia-Wen MoJia-Wen MoFang GuoFang GuoFang GuoFang GuoYou-Lu WenXiong CaoXiong CaoXiong CaoXiong CaoXiong CaoFrontiers Media S.A.articleO-GlcNAc transferaseastrocytemedial prefrontal cortexproteomicpost-translational modificationNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Molecular Neuroscience, Vol 14 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
O-GlcNAc transferase astrocyte medial prefrontal cortex proteomic post-translational modification Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 |
| spellingShingle |
O-GlcNAc transferase astrocyte medial prefrontal cortex proteomic post-translational modification Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Jun Fan Jun Fan Jun Fan Jun Fan Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Ran Mo Ran Mo Ran Mo Ran Mo Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Jing Ren Jing Ren Jing Ren Jing Ren Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Fang Guo Fang Guo Fang Guo Fang Guo You-Lu Wen Xiong Cao Xiong Cao Xiong Cao Xiong Cao Xiong Cao Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| description |
The medial prefrontal cortex (mPFC), a key part of the brain networks that are closely related to the regulation of behavior, acts as a key regulator in emotion, social cognition, and decision making. Astrocytes are the majority cell type of glial cells, which play a significant role in a number of processes and establish a suitable environment for the functioning of neurons, including the brain energy metabolism. Astrocyte’s dysfunction in the mPFC has been implicated in various neuropsychiatric disorders. Glucose is a major energy source in the brain. In glucose metabolism, part of glucose is used to convert UDP-GlcNAc as a donor molecule for O-GlcNAcylation, which is controlled by a group of enzymes, O-GlcNAc transferase enzyme (OGT), and O-GlcNAcase (OGA). However, the role of O-GlcNAcylation in astrocytes is almost completely unknown. Our research showed that astrocytic OGT could influence the expression of proteins in the mPFC. Most of these altered proteins participate in metabolic processes, transferase activity, and biosynthetic processes. GFAP, an astrocyte maker, was increased after OGT deletion. These results provide a framework for further study on the role of astrocytic OGT/O-GlcNAcylation in the mPFC. |
| format |
article |
| author |
Jun Fan Jun Fan Jun Fan Jun Fan Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Ran Mo Ran Mo Ran Mo Ran Mo Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Jing Ren Jing Ren Jing Ren Jing Ren Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Fang Guo Fang Guo Fang Guo Fang Guo You-Lu Wen Xiong Cao Xiong Cao Xiong Cao Xiong Cao Xiong Cao |
| author_facet |
Jun Fan Jun Fan Jun Fan Jun Fan Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Qiu-Ling Zhong Ran Mo Ran Mo Ran Mo Ran Mo Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Cheng-Lin Lu Jing Ren Jing Ren Jing Ren Jing Ren Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Jia-Wen Mo Fang Guo Fang Guo Fang Guo Fang Guo You-Lu Wen Xiong Cao Xiong Cao Xiong Cao Xiong Cao Xiong Cao |
| author_sort |
Jun Fan |
| title |
Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| title_short |
Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| title_full |
Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| title_fullStr |
Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| title_full_unstemmed |
Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex |
| title_sort |
proteomic profiling of astrocytic o-glcnac transferase-related proteins in the medial prefrontal cortex |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/9341dd5066144fe59e6c2119a90df68f |
| work_keys_str_mv |
AT junfan proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT junfan proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT junfan proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT junfan proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT qiulingzhong proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT qiulingzhong proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT qiulingzhong proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT qiulingzhong proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT ranmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT ranmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT ranmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT ranmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT chenglinlu proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT chenglinlu proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT chenglinlu proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT chenglinlu proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jingren proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jingren proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jingren proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jingren proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jiawenmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jiawenmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jiawenmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT jiawenmo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT fangguo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT fangguo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT fangguo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT fangguo proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT youluwen proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT xiongcao proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT xiongcao proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT xiongcao proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT xiongcao proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex AT xiongcao proteomicprofilingofastrocyticoglcnactransferaserelatedproteinsinthemedialprefrontalcortex |
| _version_ |
1718445527176052736 |