Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.

Defensins are a group of cationic peptides that exhibit broad-spectrum antimicrobial activity. In this study, we cloned and characterized a β-defensin from pituitary cDNA library of a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides). Interestingly, the β-defensin was shown to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun-Yan Jin, Li Zhou, Yang Wang, Zhi Li, Jiu-Gang Zhao, Qi-Ya Zhang, Jian-Fang Gui
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/934300265f1f45488ead9fd73fa8c78f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:934300265f1f45488ead9fd73fa8c78f
record_format	dspace
spelling	oai:doaj.org-article:934300265f1f45488ead9fd73fa8c78f2021-11-18T07:01:27ZAntibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.1932-620310.1371/journal.pone.0012883https://doaj.org/article/934300265f1f45488ead9fd73fa8c78f2010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21188147/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Defensins are a group of cationic peptides that exhibit broad-spectrum antimicrobial activity. In this study, we cloned and characterized a β-defensin from pituitary cDNA library of a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides). Interestingly, the β-defensin was shown to be dominantly expressed in pituitary and testis by RT-PCR and Western blot analysis, and its transcript level is significantly upregulated in reproduction organs from intersexual gonad to testis during the natural and artificial sex reversal. Promoter sequence and the responsible activity region analyses revealed the pituitary-specific POU1F1a transcription binding site and testis-specific SRY responsible site, and demonstrated that the pituitary-specific POU1F1a transcription binding site that locates between -180 and -208 bp is the major responsible region of grouper β-defensin promoter activity. Immunofluorescence localization observed its pituicyte expression in pituitary and spermatogonic cell expression in testis. Moreover, both in vitro antibacterial activity assay of the recombinant β-defensin and in vivo embryo microinjection of the β-defensin mRNA were shown to be effective in killing gram-negative bacteria. And, its antiviral role was also demonstrated in EPC cells transfected with the β-defensin construct. Additionally, the antibacterial activity was sensitive to concentrations of Na(+), K(+), Ca(2+) and Mg(2+). The above intriguing findings strongly suggest that the fish β-defensin might play significant roles in both innate immunity defense and reproduction endocrine regulation.Jun-Yan JinLi ZhouYang WangZhi LiJiu-Gang ZhaoQi-Ya ZhangJian-Fang GuiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e12883 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun-Yan Jin Li Zhou Yang Wang Zhi Li Jiu-Gang Zhao Qi-Ya Zhang Jian-Fang Gui Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
description	Defensins are a group of cationic peptides that exhibit broad-spectrum antimicrobial activity. In this study, we cloned and characterized a β-defensin from pituitary cDNA library of a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides). Interestingly, the β-defensin was shown to be dominantly expressed in pituitary and testis by RT-PCR and Western blot analysis, and its transcript level is significantly upregulated in reproduction organs from intersexual gonad to testis during the natural and artificial sex reversal. Promoter sequence and the responsible activity region analyses revealed the pituitary-specific POU1F1a transcription binding site and testis-specific SRY responsible site, and demonstrated that the pituitary-specific POU1F1a transcription binding site that locates between -180 and -208 bp is the major responsible region of grouper β-defensin promoter activity. Immunofluorescence localization observed its pituicyte expression in pituitary and spermatogonic cell expression in testis. Moreover, both in vitro antibacterial activity assay of the recombinant β-defensin and in vivo embryo microinjection of the β-defensin mRNA were shown to be effective in killing gram-negative bacteria. And, its antiviral role was also demonstrated in EPC cells transfected with the β-defensin construct. Additionally, the antibacterial activity was sensitive to concentrations of Na(+), K(+), Ca(2+) and Mg(2+). The above intriguing findings strongly suggest that the fish β-defensin might play significant roles in both innate immunity defense and reproduction endocrine regulation.
format	article
author	Jun-Yan Jin Li Zhou Yang Wang Zhi Li Jiu-Gang Zhao Qi-Ya Zhang Jian-Fang Gui
author_facet	Jun-Yan Jin Li Zhou Yang Wang Zhi Li Jiu-Gang Zhao Qi-Ya Zhang Jian-Fang Gui
author_sort	Jun-Yan Jin
title	Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
title_short	Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
title_full	Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
title_fullStr	Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
title_full_unstemmed	Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
title_sort	antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/934300265f1f45488ead9fd73fa8c78f
work_keys_str_mv	AT junyanjin antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT lizhou antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT yangwang antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT zhili antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT jiugangzhao antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT qiyazhang antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis AT jianfanggui antibacterialandantiviralrolesofafishbdefensinexpressedbothinpituitaryandtestis
_version_	1718424057391611904

Antibacterial and antiviral roles of a fish β-defensin expressed both in pituitary and testis.

Ejemplares similares