Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens

Sapir Karli, Juan Alfredo Ayala-Haedo, William J Feuer, Maria Fernandez, Sander Dubovy, Sara T Wester Bascom Palmer Eye Institute, Miami, FL, USA Purpose: To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibito...

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Autores principales:	Karli S, Ayala-Haedo JA, Feuer WJ, Fernandez M, Dubovy S, Wester ST
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	MMP matrix metalloproteinase prostaglandin associated periorbitopathy PAP prostaglandin analogue periorbitopathy Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/934aee6f7c534e97be7f18f3db61cd6d
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spelling	oai:doaj.org-article:934aee6f7c534e97be7f18f3db61cd6d2021-12-02T00:26:12ZEffect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens1177-5483https://doaj.org/article/934aee6f7c534e97be7f18f3db61cd6d2018-10-01T00:00:00Zhttps://www.dovepress.com/effect-of-prostaglandin-analogs-on-matrix-metalloproteinases-and-tissu-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Sapir Karli, Juan Alfredo Ayala-Haedo, William J Feuer, Maria Fernandez, Sander Dubovy, Sara T Wester Bascom Palmer Eye Institute, Miami, FL, USA Purpose: To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in levator aponeurosis resections (LAR) and conjunctiva-Muller muscle resections (CMMR).Methods: Specimens from LAR and CMMR of PA users and non-users were analyzed for tissue specific expression of MMP-3, MMP-7, MMP-9 and TIMP-2 using immunohistochemistry. PA use, marginal reflex distances, levator function and palpebral fissure were documented through chart review. The associations between MMP expression, PA exposure time and ocular characteristics were evaluated with a two-factor analysis of variance and multiple correlation analysis.Results: We observed a tissue specific pattern of expression of MMPs and TIMP-2 in relation to PA exposure between CMMR and LAR specimens. There was increased MMP-7 and TIMP-2 expression in muscle compared to collagen and adipose tissue (P≤0.005), as well as a statistically significant difference in the relationship of MMP-3, MMP-9 and TIMP-2 levels to PA exposure in the two types of muscles (all P≤0.011). Adipose tissue had a PA-dependent reduced expression of MMP-3 (P<0.022), which was seen in both LAR and CMMR. Decreased expression of MMP-3 in collagen correlated with increased dermatochalasis (P<0.045) and steatoblepharon (P<0.018).Conclusion: PA exposure may affect MMP and TIMP expression in a tissue specific manner, and decreased expression of certain MMPs in collagen correlates to increased clinical measures of prostaglandin associated periorbitopathy (PAP). Further studies with larger samples are needed to ascertain if the changes associated with PAP are due to MMP/TIMP changes or to structural changes. Keywords: MMP, matrix metalloproteinase, prostaglandin associated periorbitopathy, PAP, prostaglandin analog, periorbitopathyKarli SAyala-Haedo JAFeuer WJFernandez MDubovy SWester STDove Medical PressarticleMMPmatrix metalloproteinaseprostaglandin associated periorbitopathyPAPprostaglandin analogueperiorbitopathyOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 2039-2046 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	MMP matrix metalloproteinase prostaglandin associated periorbitopathy PAP prostaglandin analogue periorbitopathy Ophthalmology RE1-994
spellingShingle	MMP matrix metalloproteinase prostaglandin associated periorbitopathy PAP prostaglandin analogue periorbitopathy Ophthalmology RE1-994 Karli S Ayala-Haedo JA Feuer WJ Fernandez M Dubovy S Wester ST Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
description	Sapir Karli, Juan Alfredo Ayala-Haedo, William J Feuer, Maria Fernandez, Sander Dubovy, Sara T Wester Bascom Palmer Eye Institute, Miami, FL, USA Purpose: To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in levator aponeurosis resections (LAR) and conjunctiva-Muller muscle resections (CMMR).Methods: Specimens from LAR and CMMR of PA users and non-users were analyzed for tissue specific expression of MMP-3, MMP-7, MMP-9 and TIMP-2 using immunohistochemistry. PA use, marginal reflex distances, levator function and palpebral fissure were documented through chart review. The associations between MMP expression, PA exposure time and ocular characteristics were evaluated with a two-factor analysis of variance and multiple correlation analysis.Results: We observed a tissue specific pattern of expression of MMPs and TIMP-2 in relation to PA exposure between CMMR and LAR specimens. There was increased MMP-7 and TIMP-2 expression in muscle compared to collagen and adipose tissue (P≤0.005), as well as a statistically significant difference in the relationship of MMP-3, MMP-9 and TIMP-2 levels to PA exposure in the two types of muscles (all P≤0.011). Adipose tissue had a PA-dependent reduced expression of MMP-3 (P<0.022), which was seen in both LAR and CMMR. Decreased expression of MMP-3 in collagen correlated with increased dermatochalasis (P<0.045) and steatoblepharon (P<0.018).Conclusion: PA exposure may affect MMP and TIMP expression in a tissue specific manner, and decreased expression of certain MMPs in collagen correlates to increased clinical measures of prostaglandin associated periorbitopathy (PAP). Further studies with larger samples are needed to ascertain if the changes associated with PAP are due to MMP/TIMP changes or to structural changes. Keywords: MMP, matrix metalloproteinase, prostaglandin associated periorbitopathy, PAP, prostaglandin analog, periorbitopathy
format	article
author	Karli S Ayala-Haedo JA Feuer WJ Fernandez M Dubovy S Wester ST
author_facet	Karli S Ayala-Haedo JA Feuer WJ Fernandez M Dubovy S Wester ST
author_sort	Karli S
title	Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
title_short	Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
title_full	Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
title_fullStr	Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
title_full_unstemmed	Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
title_sort	effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/934aee6f7c534e97be7f18f3db61cd6d
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Effect of prostaglandin analogs on matrix metalloproteinases and tissue inhibitor of metalloproteinases in eyelid muscle specimens

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