Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.

The increased application of nanomaterials has raised the level of public concern regarding possible toxicities caused by exposure to nanostructures. The interactions of nanosized hydroxyapatite (HA) with cytochrome c and hemoglobin were investigated by zeta-potential, UV-vis, fluorescence and circu...

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Autores principales:	Zhen Xu, Ya-Lei Zhang, Cao Song, Ling-Ling Wu, Hong-Wen Gao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/934faba3db564c62a738b64043a9d4e1
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spelling	oai:doaj.org-article:934faba3db564c62a738b64043a9d4e12021-11-18T07:22:42ZInteractions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.1932-620310.1371/journal.pone.0032818https://doaj.org/article/934faba3db564c62a738b64043a9d4e12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22509249/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The increased application of nanomaterials has raised the level of public concern regarding possible toxicities caused by exposure to nanostructures. The interactions of nanosized hydroxyapatite (HA) with cytochrome c and hemoglobin were investigated by zeta-potential, UV-vis, fluorescence and circular dichroism. The experimental results indicated that the interactions were formed via charge attraction and hydrogen bond and obeyed Langmuir adsorption isotherm. The two functional proteins bridged between HA particles to aggregate into the coralloid form, where change of the secondary structure of proteins occurred. From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. The SiO(2) particles were distributed throughout the outer surface and caused jam of membrane passage, delay of the hatching time and axial malformation. Maybe owing to the oxygen free radical activity, TiO(2) caused some serious deformity characters in the cardiovascular system.Zhen XuYa-Lei ZhangCao SongLing-Ling WuHong-Wen GaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e32818 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhen Xu Ya-Lei Zhang Cao Song Ling-Ling Wu Hong-Wen Gao Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
description	The increased application of nanomaterials has raised the level of public concern regarding possible toxicities caused by exposure to nanostructures. The interactions of nanosized hydroxyapatite (HA) with cytochrome c and hemoglobin were investigated by zeta-potential, UV-vis, fluorescence and circular dichroism. The experimental results indicated that the interactions were formed via charge attraction and hydrogen bond and obeyed Langmuir adsorption isotherm. The two functional proteins bridged between HA particles to aggregate into the coralloid form, where change of the secondary structure of proteins occurred. From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. The SiO(2) particles were distributed throughout the outer surface and caused jam of membrane passage, delay of the hatching time and axial malformation. Maybe owing to the oxygen free radical activity, TiO(2) caused some serious deformity characters in the cardiovascular system.
format	article
author	Zhen Xu Ya-Lei Zhang Cao Song Ling-Ling Wu Hong-Wen Gao
author_facet	Zhen Xu Ya-Lei Zhang Cao Song Ling-Ling Wu Hong-Wen Gao
author_sort	Zhen Xu
title	Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
title_short	Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
title_full	Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
title_fullStr	Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
title_full_unstemmed	Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
title_sort	interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/934faba3db564c62a738b64043a9d4e1
work_keys_str_mv	AT zhenxu interactionsofhydroxyapatitewithproteinsanditstoxicologicaleffecttozebrafishembryosdevelopment AT yaleizhang interactionsofhydroxyapatitewithproteinsanditstoxicologicaleffecttozebrafishembryosdevelopment AT caosong interactionsofhydroxyapatitewithproteinsanditstoxicologicaleffecttozebrafishembryosdevelopment AT linglingwu interactionsofhydroxyapatitewithproteinsanditstoxicologicaleffecttozebrafishembryosdevelopment AT hongwengao interactionsofhydroxyapatitewithproteinsanditstoxicologicaleffecttozebrafishembryosdevelopment
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Interactions of hydroxyapatite with proteins and its toxicological effect to zebrafish embryos development.

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