Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis
Abstract For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial su...
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Nature Portfolio
2017
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oai:doaj.org-article:93575100676e4b12bd24e3e7f49a53b42021-12-02T11:41:19ZDirect, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis10.1038/s41598-017-01278-22045-2322https://doaj.org/article/93575100676e4b12bd24e3e7f49a53b42017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01278-2https://doaj.org/toc/2045-2322Abstract For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents. Using microscopic detection of bacterial colony formation in agarose, the total time to result from a PBCB for dRAST was only six hours for a wide range of bacterial concentrations in PBCBs. The results from the dRAST system were consistent with the results from a standard AST, broth microdilution test. In tests of clinical isolates (n = 206) composed of 16 Gram-negative species and seven Gram-positive species, the dRAST system was accurate compared to the standard broth microdilution test, with rates of 91.11% (2613/2868) categorical agreement, 6.69% (192/2868) minor error, 2.72% (50/1837) major error and 1.45% (13/896) very major error. Thus, the dRAST system can be used to rapidly identify appropriate antimicrobial agents for the treatment of blood stream infection (BSI) and antibiotic-resistant strain infections.Jungil ChoiHyun Yong JeongGi Yoon LeeSangkwon HanShinhun HanBonghwan JinTaegeun LimShin KimDong Young KimHee Chan KimEui-Chong KimSang Hoon SongTaek Soo KimSunghoon KwonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
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DOAJ |
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Medicine R Science Q |
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Medicine R Science Q Jungil Choi Hyun Yong Jeong Gi Yoon Lee Sangkwon Han Shinhun Han Bonghwan Jin Taegeun Lim Shin Kim Dong Young Kim Hee Chan Kim Eui-Chong Kim Sang Hoon Song Taek Soo Kim Sunghoon Kwon Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| description |
Abstract For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents. Using microscopic detection of bacterial colony formation in agarose, the total time to result from a PBCB for dRAST was only six hours for a wide range of bacterial concentrations in PBCBs. The results from the dRAST system were consistent with the results from a standard AST, broth microdilution test. In tests of clinical isolates (n = 206) composed of 16 Gram-negative species and seven Gram-positive species, the dRAST system was accurate compared to the standard broth microdilution test, with rates of 91.11% (2613/2868) categorical agreement, 6.69% (192/2868) minor error, 2.72% (50/1837) major error and 1.45% (13/896) very major error. Thus, the dRAST system can be used to rapidly identify appropriate antimicrobial agents for the treatment of blood stream infection (BSI) and antibiotic-resistant strain infections. |
| format |
article |
| author |
Jungil Choi Hyun Yong Jeong Gi Yoon Lee Sangkwon Han Shinhun Han Bonghwan Jin Taegeun Lim Shin Kim Dong Young Kim Hee Chan Kim Eui-Chong Kim Sang Hoon Song Taek Soo Kim Sunghoon Kwon |
| author_facet |
Jungil Choi Hyun Yong Jeong Gi Yoon Lee Sangkwon Han Shinhun Han Bonghwan Jin Taegeun Lim Shin Kim Dong Young Kim Hee Chan Kim Eui-Chong Kim Sang Hoon Song Taek Soo Kim Sunghoon Kwon |
| author_sort |
Jungil Choi |
| title |
Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| title_short |
Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| title_full |
Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| title_fullStr |
Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| title_full_unstemmed |
Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| title_sort |
direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/93575100676e4b12bd24e3e7f49a53b4 |
| work_keys_str_mv |
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