Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.

Mitochondria from diverse phyla, including protozoa, fungi, higher plants, and humans, import tRNAs from the cytosol in order to ensure proper mitochondrial translation. Despite the broad occurrence of this process, our understanding of tRNA import mechanisms is fragmentary, and crucial questions ab...

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Autores principales:	Thalia Salinas, Francéline Duby, Véronique Larosa, Nadine Coosemans, Nathalie Bonnefoy, Patrick Motte, Laurence Maréchal-Drouard, Claire Remacle
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/9360c13dc17c4b23be31395fe110b0fb
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spelling	oai:doaj.org-article:9360c13dc17c4b23be31395fe110b0fb2021-11-18T06:18:06ZCo-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.1553-73901553-740410.1371/journal.pgen.1002946https://doaj.org/article/9360c13dc17c4b23be31395fe110b0fb2012-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028354/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Mitochondria from diverse phyla, including protozoa, fungi, higher plants, and humans, import tRNAs from the cytosol in order to ensure proper mitochondrial translation. Despite the broad occurrence of this process, our understanding of tRNA import mechanisms is fragmentary, and crucial questions about their regulation remain unanswered. In the unicellular green alga Chlamydomonas, a precise correlation was found between the mitochondrial codon usage and the nature and amount of imported tRNAs. This led to the hypothesis that tRNA import might be a dynamic process able to adapt to the mitochondrial genome content. By manipulating the Chlamydomonas mitochondrial genome, we introduced point mutations in order to modify its codon usage. We find that the codon usage modification results in reduced levels of mitochondrial translation as well as in subsequent decreased levels and activities of respiratory complexes. These effects are linked to the consequential limitations of the pool of tRNAs in mitochondria. This indicates that tRNA mitochondrial import cannot be rapidly regulated in response to a novel genetic context and thus does not appear to be a dynamic process. It rather suggests that the steady-state levels of imported tRNAs in mitochondria result from a co-evolutive adaptation between the tRNA import mechanism and the requirements of the mitochondrial translation machinery.Thalia SalinasFrancéline DubyVéronique LarosaNadine CoosemansNathalie BonnefoyPatrick MotteLaurence Maréchal-DrouardClaire RemaclePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 8, Iss 9, p e1002946 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Thalia Salinas Francéline Duby Véronique Larosa Nadine Coosemans Nathalie Bonnefoy Patrick Motte Laurence Maréchal-Drouard Claire Remacle Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
description	Mitochondria from diverse phyla, including protozoa, fungi, higher plants, and humans, import tRNAs from the cytosol in order to ensure proper mitochondrial translation. Despite the broad occurrence of this process, our understanding of tRNA import mechanisms is fragmentary, and crucial questions about their regulation remain unanswered. In the unicellular green alga Chlamydomonas, a precise correlation was found between the mitochondrial codon usage and the nature and amount of imported tRNAs. This led to the hypothesis that tRNA import might be a dynamic process able to adapt to the mitochondrial genome content. By manipulating the Chlamydomonas mitochondrial genome, we introduced point mutations in order to modify its codon usage. We find that the codon usage modification results in reduced levels of mitochondrial translation as well as in subsequent decreased levels and activities of respiratory complexes. These effects are linked to the consequential limitations of the pool of tRNAs in mitochondria. This indicates that tRNA mitochondrial import cannot be rapidly regulated in response to a novel genetic context and thus does not appear to be a dynamic process. It rather suggests that the steady-state levels of imported tRNAs in mitochondria result from a co-evolutive adaptation between the tRNA import mechanism and the requirements of the mitochondrial translation machinery.
format	article
author	Thalia Salinas Francéline Duby Véronique Larosa Nadine Coosemans Nathalie Bonnefoy Patrick Motte Laurence Maréchal-Drouard Claire Remacle
author_facet	Thalia Salinas Francéline Duby Véronique Larosa Nadine Coosemans Nathalie Bonnefoy Patrick Motte Laurence Maréchal-Drouard Claire Remacle
author_sort	Thalia Salinas
title	Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
title_short	Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
title_full	Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
title_fullStr	Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
title_full_unstemmed	Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
title_sort	co-evolution of mitochondrial trna import and codon usage determines translational efficiency in the green alga chlamydomonas.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9360c13dc17c4b23be31395fe110b0fb
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Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.

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