A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China.
An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an out...
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2012
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oai:doaj.org-article:9363875088b745fea927e4d69f5729772021-11-18T07:20:25ZA novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China.1932-620310.1371/journal.pone.0036144https://doaj.org/article/9363875088b745fea927e4d69f5729772012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558360/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.Yanwen XiongPing WangRuiting LanChangyun YeHua WangJun RenHuaiqi JingYiting WangZhemin ZhouXuemei BaiZhigang CuiXia LuoAilan ZhaoYan WangShaomin ZhangHui SunLei WangJianguo XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e36144 (2012) |
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Medicine R Science Q |
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Medicine R Science Q Yanwen Xiong Ping Wang Ruiting Lan Changyun Ye Hua Wang Jun Ren Huaiqi Jing Yiting Wang Zhemin Zhou Xuemei Bai Zhigang Cui Xia Luo Ailan Zhao Yan Wang Shaomin Zhang Hui Sun Lei Wang Jianguo Xu A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| description |
An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7. |
| format |
article |
| author |
Yanwen Xiong Ping Wang Ruiting Lan Changyun Ye Hua Wang Jun Ren Huaiqi Jing Yiting Wang Zhemin Zhou Xuemei Bai Zhigang Cui Xia Luo Ailan Zhao Yan Wang Shaomin Zhang Hui Sun Lei Wang Jianguo Xu |
| author_facet |
Yanwen Xiong Ping Wang Ruiting Lan Changyun Ye Hua Wang Jun Ren Huaiqi Jing Yiting Wang Zhemin Zhou Xuemei Bai Zhigang Cui Xia Luo Ailan Zhao Yan Wang Shaomin Zhang Hui Sun Lei Wang Jianguo Xu |
| author_sort |
Yanwen Xiong |
| title |
A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| title_short |
A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| title_full |
A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| title_fullStr |
A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| title_full_unstemmed |
A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. |
| title_sort |
novel escherichia coli o157:h7 clone causing a major hemolytic uremic syndrome outbreak in china. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/9363875088b745fea927e4d69f572977 |
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