Cell Surface and Functional Features of Cortical Bone Stem Cells
The newly established mouse cortical-bone-derived stem cells (mCBSCs) are unique stem cells compared to mouse mesenchymal stem cells (mMSCs). The mCBSC-treated hearts after myocardial infarction have been reported to have greater improvement in myocardial structure and functions. In this study, we e...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9365f118b0e94f028fe399831c050fbd |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9365f118b0e94f028fe399831c050fbd |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9365f118b0e94f028fe399831c050fbd2021-11-11T17:16:34ZCell Surface and Functional Features of Cortical Bone Stem Cells10.3390/ijms2221118491422-00671661-6596https://doaj.org/article/9365f118b0e94f028fe399831c050fbd2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11849https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The newly established mouse cortical-bone-derived stem cells (mCBSCs) are unique stem cells compared to mouse mesenchymal stem cells (mMSCs). The mCBSC-treated hearts after myocardial infarction have been reported to have greater improvement in myocardial structure and functions. In this study, we examined the stemness features, cell surface glycan profiles, and paracrine functions of mCBSCs compared with mMSCs. The stemness analysis revealed that the self-renewing capacity of mCBSCs was greater than mMSCs; however, the differentiation capacity of mCBSCs was limited to the chondrogenic lineage among three types of cells (adipocyte, osteoblast, chondrocyte). The cell surface glycan profiles by lectin array analysis revealed that α2-6sialic acid is expressed at very low levels on the cell surface of mCBSCs compared with that on mMSCs. In contrast, the lactosamine (Galβ1-4GlcNAc) structure, poly lactosamine- or poly <i>N</i>-acetylglucosamine structure, and α2-3sialic acid on both <i>N</i>- and <i>O</i>-glycans were more highly expressed in mCBSCs. Moreover, we found that mCBSCs secrete a greater amount of TGF-β1 compared to mMSCs, and that the TGF-β1 contributed to the self-migration of mCBSCs and activation of fibroblasts. Together, these results suggest that unique characteristics in mCBSCs compared to mMSCs may lead to advanced utility of mCBSCs for cardiac and noncardiac repair.Norihiko SasakiYoko ItakuraSadia MohsinTomoaki IshigamiHajime KuboYumi ChibaMDPI AGarticlecortical bone stem cellsstemnessglycan profileTGF-β1Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11849, p 11849 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
cortical bone stem cells stemness glycan profile TGF-β1 Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
cortical bone stem cells stemness glycan profile TGF-β1 Biology (General) QH301-705.5 Chemistry QD1-999 Norihiko Sasaki Yoko Itakura Sadia Mohsin Tomoaki Ishigami Hajime Kubo Yumi Chiba Cell Surface and Functional Features of Cortical Bone Stem Cells |
| description |
The newly established mouse cortical-bone-derived stem cells (mCBSCs) are unique stem cells compared to mouse mesenchymal stem cells (mMSCs). The mCBSC-treated hearts after myocardial infarction have been reported to have greater improvement in myocardial structure and functions. In this study, we examined the stemness features, cell surface glycan profiles, and paracrine functions of mCBSCs compared with mMSCs. The stemness analysis revealed that the self-renewing capacity of mCBSCs was greater than mMSCs; however, the differentiation capacity of mCBSCs was limited to the chondrogenic lineage among three types of cells (adipocyte, osteoblast, chondrocyte). The cell surface glycan profiles by lectin array analysis revealed that α2-6sialic acid is expressed at very low levels on the cell surface of mCBSCs compared with that on mMSCs. In contrast, the lactosamine (Galβ1-4GlcNAc) structure, poly lactosamine- or poly <i>N</i>-acetylglucosamine structure, and α2-3sialic acid on both <i>N</i>- and <i>O</i>-glycans were more highly expressed in mCBSCs. Moreover, we found that mCBSCs secrete a greater amount of TGF-β1 compared to mMSCs, and that the TGF-β1 contributed to the self-migration of mCBSCs and activation of fibroblasts. Together, these results suggest that unique characteristics in mCBSCs compared to mMSCs may lead to advanced utility of mCBSCs for cardiac and noncardiac repair. |
| format |
article |
| author |
Norihiko Sasaki Yoko Itakura Sadia Mohsin Tomoaki Ishigami Hajime Kubo Yumi Chiba |
| author_facet |
Norihiko Sasaki Yoko Itakura Sadia Mohsin Tomoaki Ishigami Hajime Kubo Yumi Chiba |
| author_sort |
Norihiko Sasaki |
| title |
Cell Surface and Functional Features of Cortical Bone Stem Cells |
| title_short |
Cell Surface and Functional Features of Cortical Bone Stem Cells |
| title_full |
Cell Surface and Functional Features of Cortical Bone Stem Cells |
| title_fullStr |
Cell Surface and Functional Features of Cortical Bone Stem Cells |
| title_full_unstemmed |
Cell Surface and Functional Features of Cortical Bone Stem Cells |
| title_sort |
cell surface and functional features of cortical bone stem cells |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/9365f118b0e94f028fe399831c050fbd |
| work_keys_str_mv |
AT norihikosasaki cellsurfaceandfunctionalfeaturesofcorticalbonestemcells AT yokoitakura cellsurfaceandfunctionalfeaturesofcorticalbonestemcells AT sadiamohsin cellsurfaceandfunctionalfeaturesofcorticalbonestemcells AT tomoakiishigami cellsurfaceandfunctionalfeaturesofcorticalbonestemcells AT hajimekubo cellsurfaceandfunctionalfeaturesofcorticalbonestemcells AT yumichiba cellsurfaceandfunctionalfeaturesofcorticalbonestemcells |
| _version_ |
1718432131947954176 |