Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies

Advanced microbiome analysis relies on sequencing of short DNA fragments from microorganisms like bacteria, fungi, and viruses. More recently, long fragment DNA sequencing of 3rd generation sequencing has gained increasing importance and can be rapidly conducted within a few hours due to its potent...

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Autores principales: Christoph Ammer-Herrmenau, Nina Pfisterer, Tim van den Berg, Ivana Gavrilova, Ahmad Amanzada, Shiv K. Singh, Alaa Khalil, Rohia Alili, Eugeni Belda, Karine Clement, Ahmed Abd El Wahed, ElSagad Eltayeb Gady, Martin Haubrock, Tim Beißbarth, Volker Ellenrieder, Albrecht Neesse
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Publicado: American Society for Microbiology 2021
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Acceso en línea:https://doaj.org/article/9368c7ba407642fabca0ad62801aa4de
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spelling oai:doaj.org-article:9368c7ba407642fabca0ad62801aa4de2021-12-02T17:49:14ZComprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies2379-507710.1128/mSystems.00750-21https://doaj.org/article/9368c7ba407642fabca0ad62801aa4de2021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00750-21https://doaj.org/toc/2379-5077 Advanced microbiome analysis relies on sequencing of short DNA fragments from microorganisms like bacteria, fungi, and viruses. More recently, long fragment DNA sequencing of 3rd generation sequencing has gained increasing importance and can be rapidly conducted within a few hours due to its potential real-time sequencing.Christoph Ammer-HerrmenauNina PfistererTim van den BergIvana GavrilovaAhmad AmanzadaShiv K. SinghAlaa KhalilRohia AliliEugeni BeldaKarine ClementAhmed Abd El WahedElSagad Eltayeb GadyMartin HaubrockTim BeißbarthVolker EllenriederAlbrecht NeesseAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 4 (2021)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Christoph Ammer-Herrmenau
Nina Pfisterer
Tim van den Berg
Ivana Gavrilova
Ahmad Amanzada
Shiv K. Singh
Alaa Khalil
Rohia Alili
Eugeni Belda
Karine Clement
Ahmed Abd El Wahed
ElSagad Eltayeb Gady
Martin Haubrock
Tim Beißbarth
Volker Ellenrieder
Albrecht Neesse
Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
description Advanced microbiome analysis relies on sequencing of short DNA fragments from microorganisms like bacteria, fungi, and viruses. More recently, long fragment DNA sequencing of 3rd generation sequencing has gained increasing importance and can be rapidly conducted within a few hours due to its potential real-time sequencing.
format article
author Christoph Ammer-Herrmenau
Nina Pfisterer
Tim van den Berg
Ivana Gavrilova
Ahmad Amanzada
Shiv K. Singh
Alaa Khalil
Rohia Alili
Eugeni Belda
Karine Clement
Ahmed Abd El Wahed
ElSagad Eltayeb Gady
Martin Haubrock
Tim Beißbarth
Volker Ellenrieder
Albrecht Neesse
author_facet Christoph Ammer-Herrmenau
Nina Pfisterer
Tim van den Berg
Ivana Gavrilova
Ahmad Amanzada
Shiv K. Singh
Alaa Khalil
Rohia Alili
Eugeni Belda
Karine Clement
Ahmed Abd El Wahed
ElSagad Eltayeb Gady
Martin Haubrock
Tim Beißbarth
Volker Ellenrieder
Albrecht Neesse
author_sort Christoph Ammer-Herrmenau
title Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
title_short Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
title_full Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
title_fullStr Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
title_full_unstemmed Comprehensive Wet-Bench and Bioinformatics Workflow for Complex Microbiota Using Oxford Nanopore Technologies
title_sort comprehensive wet-bench and bioinformatics workflow for complex microbiota using oxford nanopore technologies
publisher American Society for Microbiology
publishDate 2021
url https://doaj.org/article/9368c7ba407642fabca0ad62801aa4de
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