Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato

Plants have evolved a two-layered immune system consisting of pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). PTI and ETI are functionally linked, but also have distinct characteristics. Unraveling how these immune systems coordinate plant responses against pathogens is cruci...

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Autores principales:	Juanjuan Yu, Juan M. Gonzalez, Zhiping Dong, Qianru Shan, Bowen Tan, Jin Koh, Tong Zhang, Ning Zhu, Craig Dufresne, Gregory B. Martin, Sixue Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tomato pattern-triggered immunity effector-triggered immunity proteomics phosphoproteomics Pseudomonas syringae Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/9369beebb6e94ab08171db64754633b3
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spelling	oai:doaj.org-article:9369beebb6e94ab08171db64754633b32021-12-03T05:10:05ZIntegrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato1664-462X10.3389/fpls.2021.768693https://doaj.org/article/9369beebb6e94ab08171db64754633b32021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.768693/fullhttps://doaj.org/toc/1664-462XPlants have evolved a two-layered immune system consisting of pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). PTI and ETI are functionally linked, but also have distinct characteristics. Unraveling how these immune systems coordinate plant responses against pathogens is crucial for understanding the regulatory mechanisms underlying plant defense. Here we report integrative proteomic and phosphoproteomic analyses of the tomato-Pseudomonas syringae (Pst) pathosystem with different Pst mutants that allow the dissection of PTI and ETI. A total of 225 proteins and 79 phosphopeptides differentially accumulated in tomato leaves during Pst infection. The abundances of many proteins and phosphoproteins changed during PTI or ETI, and some responses were triggered by both PTI and ETI. For most proteins, the ETI response was more robust than the PTI response. The patterns of protein abundance and phosphorylation changes revealed key regulators involved in Ca2+ signaling, mitogen-activated protein kinase cascades, reversible protein phosphorylation, reactive oxygen species (ROS) and redox homeostasis, transcription and protein turnover, transport and trafficking, cell wall remodeling, hormone biosynthesis and signaling, suggesting their common or specific roles in PTI and/or ETI. A NAC (NAM, ATAF, and CUC family) domain protein and lipid particle serine esterase, two PTI-specific genes identified from previous transcriptomic work, were not detected as differentially regulated at the protein level and were not induced by PTI. Based on integrative transcriptomics and proteomics data, as well as qRT-PCR analysis, several potential PTI and ETI-specific markers are proposed. These results provide insights into the regulatory mechanisms underlying PTI and ETI in the tomato-Pst pathosystem, and will promote future validation and application of the disease biomarkers in plant defense.Juanjuan YuJuan M. GonzalezJuan M. GonzalezZhiping DongQianru ShanBowen TanJin KohTong ZhangNing ZhuCraig DufresneGregory B. MartinGregory B. MartinSixue ChenFrontiers Media S.A.articletomatopattern-triggered immunityeffector-triggered immunityproteomicsphosphoproteomicsPseudomonas syringaePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tomato pattern-triggered immunity effector-triggered immunity proteomics phosphoproteomics Pseudomonas syringae Plant culture SB1-1110
spellingShingle	tomato pattern-triggered immunity effector-triggered immunity proteomics phosphoproteomics Pseudomonas syringae Plant culture SB1-1110 Juanjuan Yu Juan M. Gonzalez Juan M. Gonzalez Zhiping Dong Qianru Shan Bowen Tan Jin Koh Tong Zhang Ning Zhu Craig Dufresne Gregory B. Martin Gregory B. Martin Sixue Chen Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
description	Plants have evolved a two-layered immune system consisting of pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). PTI and ETI are functionally linked, but also have distinct characteristics. Unraveling how these immune systems coordinate plant responses against pathogens is crucial for understanding the regulatory mechanisms underlying plant defense. Here we report integrative proteomic and phosphoproteomic analyses of the tomato-Pseudomonas syringae (Pst) pathosystem with different Pst mutants that allow the dissection of PTI and ETI. A total of 225 proteins and 79 phosphopeptides differentially accumulated in tomato leaves during Pst infection. The abundances of many proteins and phosphoproteins changed during PTI or ETI, and some responses were triggered by both PTI and ETI. For most proteins, the ETI response was more robust than the PTI response. The patterns of protein abundance and phosphorylation changes revealed key regulators involved in Ca2+ signaling, mitogen-activated protein kinase cascades, reversible protein phosphorylation, reactive oxygen species (ROS) and redox homeostasis, transcription and protein turnover, transport and trafficking, cell wall remodeling, hormone biosynthesis and signaling, suggesting their common or specific roles in PTI and/or ETI. A NAC (NAM, ATAF, and CUC family) domain protein and lipid particle serine esterase, two PTI-specific genes identified from previous transcriptomic work, were not detected as differentially regulated at the protein level and were not induced by PTI. Based on integrative transcriptomics and proteomics data, as well as qRT-PCR analysis, several potential PTI and ETI-specific markers are proposed. These results provide insights into the regulatory mechanisms underlying PTI and ETI in the tomato-Pst pathosystem, and will promote future validation and application of the disease biomarkers in plant defense.
format	article
author	Juanjuan Yu Juan M. Gonzalez Juan M. Gonzalez Zhiping Dong Qianru Shan Bowen Tan Jin Koh Tong Zhang Ning Zhu Craig Dufresne Gregory B. Martin Gregory B. Martin Sixue Chen
author_facet	Juanjuan Yu Juan M. Gonzalez Juan M. Gonzalez Zhiping Dong Qianru Shan Bowen Tan Jin Koh Tong Zhang Ning Zhu Craig Dufresne Gregory B. Martin Gregory B. Martin Sixue Chen
author_sort	Juanjuan Yu
title	Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
title_short	Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
title_full	Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
title_fullStr	Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
title_full_unstemmed	Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato
title_sort	integrative proteomic and phosphoproteomic analyses of pattern- and effector-triggered immunity in tomato
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9369beebb6e94ab08171db64754633b3
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Integrative Proteomic and Phosphoproteomic Analyses of Pattern- and Effector-Triggered Immunity in Tomato

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