Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

Multicolour images are difficult to acquire with large-scale microscopy approaches. Here the authors present a microtome-assisted microscope capable of trichromatic two-photon excitation and label-free nonlinear modalities based on wavelength mixing, and use it to analyze astrocyte morphology and ne...

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Autores principales:	Lamiae Abdeladim, Katherine S. Matho, Solène Clavreul, Pierre Mahou, Jean-Marc Sintes, Xavier Solinas, Ignacio Arganda-Carreras, Stephen G. Turney, Jeff W. Lichtman, Anatole Chessel, Alexis-Pierre Bemelmans, Karine Loulier, Willy Supatto, Jean Livet, Emmanuel Beaurepaire
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/936f22a7c3dd47068d7ddbe4fade0e58
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spelling	oai:doaj.org-article:936f22a7c3dd47068d7ddbe4fade0e582021-12-02T16:58:25ZMulticolor multiscale brain imaging with chromatic multiphoton serial microscopy10.1038/s41467-019-09552-92041-1723https://doaj.org/article/936f22a7c3dd47068d7ddbe4fade0e582019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09552-9https://doaj.org/toc/2041-1723Multicolour images are difficult to acquire with large-scale microscopy approaches. Here the authors present a microtome-assisted microscope capable of trichromatic two-photon excitation and label-free nonlinear modalities based on wavelength mixing, and use it to analyze astrocyte morphology and neuronal projections in thick brain samples.Lamiae AbdeladimKatherine S. MathoSolène ClavreulPierre MahouJean-Marc SintesXavier SolinasIgnacio Arganda-CarrerasStephen G. TurneyJeff W. LichtmanAnatole ChesselAlexis-Pierre BemelmansKarine LoulierWilly SupattoJean LivetEmmanuel BeaurepaireNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lamiae Abdeladim Katherine S. Matho Solène Clavreul Pierre Mahou Jean-Marc Sintes Xavier Solinas Ignacio Arganda-Carreras Stephen G. Turney Jeff W. Lichtman Anatole Chessel Alexis-Pierre Bemelmans Karine Loulier Willy Supatto Jean Livet Emmanuel Beaurepaire Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
description	Multicolour images are difficult to acquire with large-scale microscopy approaches. Here the authors present a microtome-assisted microscope capable of trichromatic two-photon excitation and label-free nonlinear modalities based on wavelength mixing, and use it to analyze astrocyte morphology and neuronal projections in thick brain samples.
format	article
author	Lamiae Abdeladim Katherine S. Matho Solène Clavreul Pierre Mahou Jean-Marc Sintes Xavier Solinas Ignacio Arganda-Carreras Stephen G. Turney Jeff W. Lichtman Anatole Chessel Alexis-Pierre Bemelmans Karine Loulier Willy Supatto Jean Livet Emmanuel Beaurepaire
author_facet	Lamiae Abdeladim Katherine S. Matho Solène Clavreul Pierre Mahou Jean-Marc Sintes Xavier Solinas Ignacio Arganda-Carreras Stephen G. Turney Jeff W. Lichtman Anatole Chessel Alexis-Pierre Bemelmans Karine Loulier Willy Supatto Jean Livet Emmanuel Beaurepaire
author_sort	Lamiae Abdeladim
title	Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
title_short	Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
title_full	Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
title_fullStr	Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
title_full_unstemmed	Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
title_sort	multicolor multiscale brain imaging with chromatic multiphoton serial microscopy
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/936f22a7c3dd47068d7ddbe4fade0e58
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Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

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