Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.

Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We sh...

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Autores principales:	Arnaud Lagorce, Aude Fourçans, Murielle Dutertre, Brice Bouyssiere, Yvan Zivanovic, Fabrice Confalonieri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/937167528acd4ac4950acaef78377428
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spelling	oai:doaj.org-article:937167528acd4ac4950acaef783774282021-11-18T07:10:38ZGenome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.1932-620310.1371/journal.pone.0041935https://doaj.org/article/937167528acd4ac4950acaef783774282012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848664/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO(4)) and that cells exposed to 1 mM Cd exhibit a cellular Cd concentration of 67 µM. A time-dependent transcriptomic analysis using microarrays was performed at a non-toxic (100 µM) and a toxic (1 mM) Cd dose. The reliability of microarray data was strengthened by real time RT-PCR validations. Altogether, 114 Cd responsive genes were revealed and a substantial subset of genes is related to metal homeostasis, drug detoxification, re-oxidization of cofactors and ATP production. This first genome-wide expression profiling study of archaeal cells challenged with Cd showed that T. gammatolerans withstands induced stress through pathways observed in both prokaryotes and eukaryotes but also through new and original strategies. T. gammatolerans cells challenged with 1 mM Cd basically promote: 1) the induction of several transporter/permease encoding genes, probably to detoxify the cell; 2) the upregulation of Fe transporters encoding genes to likely compensate Cd damages in iron-containing proteins; 3) the induction of membrane-bound hydrogenase (Mbh) and membrane-bound hydrogenlyase (Mhy2) subunits encoding genes involved in recycling reduced cofactors and/or in proton translocation for energy production. By contrast to other organisms, redox homeostasis genes appear constitutively expressed and only a few genes encoding DNA repair proteins are regulated. We compared the expression of 27 Cd responsive genes in other stress conditions (Zn, Ni, heat shock, γ-rays), and showed that the Cd transcriptional pattern is comparable to other metal stress transcriptional responses (Cd, Zn, Ni) but not to a general stress response.Arnaud LagorceAude FourçansMurielle DutertreBrice BouyssiereYvan ZivanovicFabrice ConfalonieriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41935 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arnaud Lagorce Aude Fourçans Murielle Dutertre Brice Bouyssiere Yvan Zivanovic Fabrice Confalonieri Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
description	Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO(4)) and that cells exposed to 1 mM Cd exhibit a cellular Cd concentration of 67 µM. A time-dependent transcriptomic analysis using microarrays was performed at a non-toxic (100 µM) and a toxic (1 mM) Cd dose. The reliability of microarray data was strengthened by real time RT-PCR validations. Altogether, 114 Cd responsive genes were revealed and a substantial subset of genes is related to metal homeostasis, drug detoxification, re-oxidization of cofactors and ATP production. This first genome-wide expression profiling study of archaeal cells challenged with Cd showed that T. gammatolerans withstands induced stress through pathways observed in both prokaryotes and eukaryotes but also through new and original strategies. T. gammatolerans cells challenged with 1 mM Cd basically promote: 1) the induction of several transporter/permease encoding genes, probably to detoxify the cell; 2) the upregulation of Fe transporters encoding genes to likely compensate Cd damages in iron-containing proteins; 3) the induction of membrane-bound hydrogenase (Mbh) and membrane-bound hydrogenlyase (Mhy2) subunits encoding genes involved in recycling reduced cofactors and/or in proton translocation for energy production. By contrast to other organisms, redox homeostasis genes appear constitutively expressed and only a few genes encoding DNA repair proteins are regulated. We compared the expression of 27 Cd responsive genes in other stress conditions (Zn, Ni, heat shock, γ-rays), and showed that the Cd transcriptional pattern is comparable to other metal stress transcriptional responses (Cd, Zn, Ni) but not to a general stress response.
format	article
author	Arnaud Lagorce Aude Fourçans Murielle Dutertre Brice Bouyssiere Yvan Zivanovic Fabrice Confalonieri
author_facet	Arnaud Lagorce Aude Fourçans Murielle Dutertre Brice Bouyssiere Yvan Zivanovic Fabrice Confalonieri
author_sort	Arnaud Lagorce
title	Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
title_short	Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
title_full	Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
title_fullStr	Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
title_full_unstemmed	Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.
title_sort	genome-wide transcriptional response of the archaeon thermococcus gammatolerans to cadmium.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/937167528acd4ac4950acaef78377428
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Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.

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