Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin

Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSC...

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Autores principales:	Yanchang Liu, Wendan Cheng, Yao Zhao, Liang Gao, Yongyun Chang, Zhicheng Tong, Huiwu Li, Juehua Jing
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bone marrow stromal cells TiO2 nanotube osteogenic differentiation GCN5 HAT Wnt/β-catenin signaling pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/9371e015434048238bccf2406e530ea7
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spelling	oai:doaj.org-article:9371e015434048238bccf2406e530ea72021-11-15T05:10:44ZCyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin2296-418510.3389/fbioe.2021.735949https://doaj.org/article/9371e015434048238bccf2406e530ea72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.735949/fullhttps://doaj.org/toc/2296-4185Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO2 nanotubes were subjected to cyclic mechanical stress, and the related mechanisms were explored. The results of alkaline phosphatase staining, real-time PCR, and Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO2 nanotubes through Wnt/β-catenin. As an important member of the histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of the ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO2 nanotubes was enhanced under the stimulation of cyclic mechanical stress, and GCN5 mediated this process through Wnt/β-catenin.Yanchang LiuWendan ChengYao ZhaoLiang GaoYongyun ChangZhicheng TongHuiwu LiJuehua JingFrontiers Media S.A.articlebone marrow stromal cellsTiO2 nanotubeosteogenic differentiationGCN5 HATWnt/β-catenin signaling pathwayBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone marrow stromal cells TiO2 nanotube osteogenic differentiation GCN5 HAT Wnt/β-catenin signaling pathway Biotechnology TP248.13-248.65
spellingShingle	bone marrow stromal cells TiO2 nanotube osteogenic differentiation GCN5 HAT Wnt/β-catenin signaling pathway Biotechnology TP248.13-248.65 Yanchang Liu Wendan Cheng Yao Zhao Liang Gao Yongyun Chang Zhicheng Tong Huiwu Li Juehua Jing Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
description	Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO2 nanotubes were subjected to cyclic mechanical stress, and the related mechanisms were explored. The results of alkaline phosphatase staining, real-time PCR, and Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO2 nanotubes through Wnt/β-catenin. As an important member of the histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of the ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO2 nanotubes was enhanced under the stimulation of cyclic mechanical stress, and GCN5 mediated this process through Wnt/β-catenin.
format	article
author	Yanchang Liu Wendan Cheng Yao Zhao Liang Gao Yongyun Chang Zhicheng Tong Huiwu Li Juehua Jing
author_facet	Yanchang Liu Wendan Cheng Yao Zhao Liang Gao Yongyun Chang Zhicheng Tong Huiwu Li Juehua Jing
author_sort	Yanchang Liu
title	Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
title_short	Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
title_full	Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
title_fullStr	Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
title_full_unstemmed	Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin
title_sort	cyclic mechanical strain regulates osteoblastic differentiation of mesenchymal stem cells on tio2 nanotubes through gcn5 and wnt/β-catenin
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9371e015434048238bccf2406e530ea7
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Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin

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