Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.

Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na⁺/H⁺ antiporter is investigated for the first time, by a combination of experimental and computational methodologies...

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Autores principales:	Assaf Ganoth, Raphael Alhadeff, Dovrat Kohen, Isaiah T Arkin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9377b0edc1904d80b066ec144b7d1476
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spelling	oai:doaj.org-article:9377b0edc1904d80b066ec144b7d14762021-11-18T07:34:20ZCharacterization of the Na⁺/H⁺ antiporter from Yersinia pestis.1932-620310.1371/journal.pone.0026115https://doaj.org/article/9377b0edc1904d80b066ec144b7d14762011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22102858/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na⁺/H⁺ antiporter is investigated for the first time, by a combination of experimental and computational methodologies. We determined the protein's substrate specificity and pH dependence by fluorescence measurements in everted membrane vesicles. Subsequently, we constructed a model of the protein's structure and validated the model using molecular dynamics simulations. Taken together, better understanding of the Yersinia pestis Na⁺/H⁺ antiporter's structure-function relationship may assist in studies on ion transport, mechanism of action and designing specific blockers of Na⁺/H⁺ antiporter to help in fighting Yersinia pestis -associated infections. We hope that our model will prove useful both from mechanistic and pharmaceutical perspectives.Assaf GanothRaphael AlhadeffDovrat KohenIsaiah T ArkinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e26115 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Assaf Ganoth Raphael Alhadeff Dovrat Kohen Isaiah T Arkin Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
description	Yersinia pestis, the bacterium that historically accounts for the Black Death epidemics, has nowadays gained new attention as a possible biological warfare agent. In this study, its Na⁺/H⁺ antiporter is investigated for the first time, by a combination of experimental and computational methodologies. We determined the protein's substrate specificity and pH dependence by fluorescence measurements in everted membrane vesicles. Subsequently, we constructed a model of the protein's structure and validated the model using molecular dynamics simulations. Taken together, better understanding of the Yersinia pestis Na⁺/H⁺ antiporter's structure-function relationship may assist in studies on ion transport, mechanism of action and designing specific blockers of Na⁺/H⁺ antiporter to help in fighting Yersinia pestis -associated infections. We hope that our model will prove useful both from mechanistic and pharmaceutical perspectives.
format	article
author	Assaf Ganoth Raphael Alhadeff Dovrat Kohen Isaiah T Arkin
author_facet	Assaf Ganoth Raphael Alhadeff Dovrat Kohen Isaiah T Arkin
author_sort	Assaf Ganoth
title	Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
title_short	Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
title_full	Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
title_fullStr	Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
title_full_unstemmed	Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.
title_sort	characterization of the na⁺/h⁺ antiporter from yersinia pestis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/9377b0edc1904d80b066ec144b7d1476
work_keys_str_mv	AT assafganoth characterizationofthenahantiporterfromyersiniapestis AT raphaelalhadeff characterizationofthenahantiporterfromyersiniapestis AT dovratkohen characterizationofthenahantiporterfromyersiniapestis AT isaiahtarkin characterizationofthenahantiporterfromyersiniapestis
_version_	1718423301126094848

Characterization of the Na⁺/H⁺ antiporter from Yersinia pestis.

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