Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

Abstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and...

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Autores principales:	Aline Q. Rodrigues, Victor L. Picolo, Jair T. Goulart, Isabella M. G. Silva, Rayane B. Ribeiro, Beatriz A. Aguiar, Yasmin B. Ferreira, Daniela M. Oliveira, Carolina M. Lucci, Andreza F. de Bem, Fernanda Paulini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/937c0480c522419c99b23c33d022ca12
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spelling	oai:doaj.org-article:937c0480c522419c99b23c33d022ca122021-11-08T10:52:50ZMetabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry10.1038/s41598-021-01082-z2045-2322https://doaj.org/article/937c0480c522419c99b23c33d022ca122021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01082-zhttps://doaj.org/toc/2045-2322Abstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.Aline Q. RodriguesVictor L. PicoloJair T. GoulartIsabella M. G. SilvaRayane B. RibeiroBeatriz A. AguiarYasmin B. FerreiraDaniela M. OliveiraCarolina M. LucciAndreza F. de BemFernanda PauliniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
description	Abstract Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.
format	article
author	Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini
author_facet	Aline Q. Rodrigues Victor L. Picolo Jair T. Goulart Isabella M. G. Silva Rayane B. Ribeiro Beatriz A. Aguiar Yasmin B. Ferreira Daniela M. Oliveira Carolina M. Lucci Andreza F. de Bem Fernanda Paulini
author_sort	Aline Q. Rodrigues
title	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
title_short	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
title_full	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
title_fullStr	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
title_full_unstemmed	Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
title_sort	metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/937c0480c522419c99b23c33d022ca12
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Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry

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