Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ

Abstract Hippo signalling pathway is an emerging signalling pathway that plays important roles in organ size control, tumorigenesis, metastasis, stress response, apoptosis, stem cell differentiation and renewal during development and tissue homeostasis. Recent studies reported that human serine/thre...

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Autores principales:	Prem Khanal, Zongchao Jia, Xiaolong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/937f5fb63a0a43d4a7328c608d924507
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spelling	oai:doaj.org-article:937f5fb63a0a43d4a7328c608d9245072021-12-02T11:40:24ZCysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ10.1038/s41598-018-21828-62045-2322https://doaj.org/article/937f5fb63a0a43d4a7328c608d9245072018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21828-6https://doaj.org/toc/2045-2322Abstract Hippo signalling pathway is an emerging signalling pathway that plays important roles in organ size control, tumorigenesis, metastasis, stress response, apoptosis, stem cell differentiation and renewal during development and tissue homeostasis. Recent studies reported that human serine/threonine protein kinase, Mst1, a core component of the Hippo pathway can be activated through formation of homodimer. However, it is still unclear whether or not other components of the Hippo pathway are also regulated through dimerization. Here we provide the first evidence that Hippo components and oncoprotein YAP2L and TAZ can form homodimer in vitro and in vivo by forming disulphide bond through cysteine residue(s). We have also shown that the homodimers of YAP2L/TAZ are more stable and showed more oncogenic behaviour than their corresponding monomers as revealed by colony formation and cell transformation assay. Since cysteine post-translational regulation plays important roles in redox signalling, tumorigenesis and drug resistance, further studies on the functional effect of this dimerization through post-translational modulation of cysteine residues in YAP2L/TAZ will provide a significant contribution to our understanding of the roles of YAP2L/TAZ in cancer development and therapy.Prem KhanalZongchao JiaXiaolong YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Prem Khanal Zongchao Jia Xiaolong Yang Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
description	Abstract Hippo signalling pathway is an emerging signalling pathway that plays important roles in organ size control, tumorigenesis, metastasis, stress response, apoptosis, stem cell differentiation and renewal during development and tissue homeostasis. Recent studies reported that human serine/threonine protein kinase, Mst1, a core component of the Hippo pathway can be activated through formation of homodimer. However, it is still unclear whether or not other components of the Hippo pathway are also regulated through dimerization. Here we provide the first evidence that Hippo components and oncoprotein YAP2L and TAZ can form homodimer in vitro and in vivo by forming disulphide bond through cysteine residue(s). We have also shown that the homodimers of YAP2L/TAZ are more stable and showed more oncogenic behaviour than their corresponding monomers as revealed by colony formation and cell transformation assay. Since cysteine post-translational regulation plays important roles in redox signalling, tumorigenesis and drug resistance, further studies on the functional effect of this dimerization through post-translational modulation of cysteine residues in YAP2L/TAZ will provide a significant contribution to our understanding of the roles of YAP2L/TAZ in cancer development and therapy.
format	article
author	Prem Khanal Zongchao Jia Xiaolong Yang
author_facet	Prem Khanal Zongchao Jia Xiaolong Yang
author_sort	Prem Khanal
title	Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
title_short	Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
title_full	Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
title_fullStr	Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
title_full_unstemmed	Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ
title_sort	cysteine residues are essential for dimerization of hippo pathway components yap2l and taz
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/937f5fb63a0a43d4a7328c608d924507
work_keys_str_mv	AT premkhanal cysteineresiduesareessentialfordimerizationofhippopathwaycomponentsyap2landtaz AT zongchaojia cysteineresiduesareessentialfordimerizationofhippopathwaycomponentsyap2landtaz AT xiaolongyang cysteineresiduesareessentialfordimerizationofhippopathwaycomponentsyap2landtaz
_version_	1718395659129716736

Cysteine residues are essential for dimerization of Hippo pathway components YAP2L and TAZ

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