Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance

Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficult...

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Autores principales:	Seungju Oh, Hyeyeon Hur, Yoonjae Kim, Seongcheol Shin, Hyunjeong Woo, Jonghoon Choi, Hyun Ho Lee
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nanoplastics LSPR peptide binding sandwich assay Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/93803f89f74341b79db27e45241a08bd
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spelling	oai:doaj.org-article:93803f89f74341b79db27e45241a08bd2021-11-25T18:30:37ZPeptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance10.3390/nano111128872079-4991https://doaj.org/article/93803f89f74341b79db27e45241a08bd2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2887https://doaj.org/toc/2079-4991Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficulties for sensing microplastics due to the limited methodologies for selectively recognizing nanoplastics. In this study, a customized gold nanoparticles (Au NPs) based localized surface plasmon resonance (LSPR) system having bio-mimicked peptide probes toward the nanoplastics was demonstrated. The specific determination through the oligo-peptide recognition was accomplished by chemical conjugation both on the LSPR chip’s 40~50 nm Au NPs and sandwiched 5 nm Au NPs, respectively. The peptide probe could selectively bind to polystyrene (PS) nanoplastics in the forms of fragmented debris by cryo-grinding. A simple UV-Vis spectrophotometer was used to identify the LSPR sensing by primarily measuring the absorbance change and shift of absorption peak. The sandwich-binding could increase the LSPR detection sensitivity up to 60% due to consecutive plasmonic effects. In addition, microwave-boiled DI water inside of a styrofoam container was tested for putative PS nanoplastics resource as a real accessible sample. The LSPR system could be a novel protocol overcoming the limitations from conventional nanoplastic detection.Seungju OhHyeyeon HurYoonjae KimSeongcheol ShinHyunjeong WooJonghoon ChoiHyun Ho LeeMDPI AGarticlenanoplasticsLSPRpeptide bindingsandwich assayChemistryQD1-999ENNanomaterials, Vol 11, Iss 2887, p 2887 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoplastics LSPR peptide binding sandwich assay Chemistry QD1-999
spellingShingle	nanoplastics LSPR peptide binding sandwich assay Chemistry QD1-999 Seungju Oh Hyeyeon Hur Yoonjae Kim Seongcheol Shin Hyunjeong Woo Jonghoon Choi Hyun Ho Lee Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
description	Recently, various waste microplastics sensors have been introduced in response to environmental and biological hazards posed by waste microplastics. In particular, the detrimental effects of nano-sized plastics or nanoplastics have been reported to be severe. Moreover, there have been many difficulties for sensing microplastics due to the limited methodologies for selectively recognizing nanoplastics. In this study, a customized gold nanoparticles (Au NPs) based localized surface plasmon resonance (LSPR) system having bio-mimicked peptide probes toward the nanoplastics was demonstrated. The specific determination through the oligo-peptide recognition was accomplished by chemical conjugation both on the LSPR chip’s 40~50 nm Au NPs and sandwiched 5 nm Au NPs, respectively. The peptide probe could selectively bind to polystyrene (PS) nanoplastics in the forms of fragmented debris by cryo-grinding. A simple UV-Vis spectrophotometer was used to identify the LSPR sensing by primarily measuring the absorbance change and shift of absorption peak. The sandwich-binding could increase the LSPR detection sensitivity up to 60% due to consecutive plasmonic effects. In addition, microwave-boiled DI water inside of a styrofoam container was tested for putative PS nanoplastics resource as a real accessible sample. The LSPR system could be a novel protocol overcoming the limitations from conventional nanoplastic detection.
format	article
author	Seungju Oh Hyeyeon Hur Yoonjae Kim Seongcheol Shin Hyunjeong Woo Jonghoon Choi Hyun Ho Lee
author_facet	Seungju Oh Hyeyeon Hur Yoonjae Kim Seongcheol Shin Hyunjeong Woo Jonghoon Choi Hyun Ho Lee
author_sort	Seungju Oh
title	Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
title_short	Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
title_full	Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
title_fullStr	Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
title_full_unstemmed	Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance
title_sort	peptide specific nanoplastic detection based on sandwich typed localized surface plasmon resonance
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/93803f89f74341b79db27e45241a08bd
work_keys_str_mv	AT seungjuoh peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT hyeyeonhur peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT yoonjaekim peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT seongcheolshin peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT hyunjeongwoo peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT jonghoonchoi peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance AT hyunholee peptidespecificnanoplasticdetectionbasedonsandwichtypedlocalizedsurfaceplasmonresonance
_version_	1718411091558531072

Peptide Specific Nanoplastic Detection Based on Sandwich Typed Localized Surface Plasmon Resonance

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