Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens

Abstract Duration of fertility, (DF) is an important functional trait in poultry production and lncRNAs have emerged as important regulators of various process including fertility. In this study we applied a genome-guided strategy to reconstruct the uterovaginal junction (UVJ) transcriptome of 14 eg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adeyinka Abiola Adetula, Lantao Gu, Chinedu Charles Nwafor, Xiaoyong Du, Shuhong Zhao, Shijun Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93816f0c019f4d6a8ecd397bf242d597
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:93816f0c019f4d6a8ecd397bf242d597
record_format	dspace
spelling	oai:doaj.org-article:93816f0c019f4d6a8ecd397bf242d5972021-12-02T15:08:37ZTranscriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens10.1038/s41598-018-31301-z2045-2322https://doaj.org/article/93816f0c019f4d6a8ecd397bf242d5972018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31301-zhttps://doaj.org/toc/2045-2322Abstract Duration of fertility, (DF) is an important functional trait in poultry production and lncRNAs have emerged as important regulators of various process including fertility. In this study we applied a genome-guided strategy to reconstruct the uterovaginal junction (UVJ) transcriptome of 14 egg-laying birds with long- and short-DF (n = 7); and sought to uncover key lncRNAs related to duration of fertility traits by RNA-sequencing technology. Examination of RNA-seq data revealed a total of 9977 lncRNAs including 2576 novel lncRNAs. Differential expression (DE) analysis of lncRNA identified 223 lncRNAs differentially expressed between the two groups. DE-lncRNA target genes prediction uncovered over 200 lncRNA target genes and functional enrichment tests predict a potential function of DE-lncRNAs. Gene ontology classification and pathway analysis revealed 8 DE-lncRNAs, with the majority of their target genes enriched in biological functions such as reproductive structure development, developmental process involved in reproduction, response to cytokine, carbohydrate binding, chromatin organization, and immune pathways. Differential expression of lncRNAs and target genes were confirmed by qPCR. Together, these results significantly expand the utility of the UVJ transcriptome and our analysis identification of key lncRNAs and their target genes regulating DF will form the baseline for understanding the molecular functions of lncRNAs regulating DF.Adeyinka Abiola AdetulaLantao GuChinedu Charles NwaforXiaoyong DuShuhong ZhaoShijun LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adeyinka Abiola Adetula Lantao Gu Chinedu Charles Nwafor Xiaoyong Du Shuhong Zhao Shijun Li Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
description	Abstract Duration of fertility, (DF) is an important functional trait in poultry production and lncRNAs have emerged as important regulators of various process including fertility. In this study we applied a genome-guided strategy to reconstruct the uterovaginal junction (UVJ) transcriptome of 14 egg-laying birds with long- and short-DF (n = 7); and sought to uncover key lncRNAs related to duration of fertility traits by RNA-sequencing technology. Examination of RNA-seq data revealed a total of 9977 lncRNAs including 2576 novel lncRNAs. Differential expression (DE) analysis of lncRNA identified 223 lncRNAs differentially expressed between the two groups. DE-lncRNA target genes prediction uncovered over 200 lncRNA target genes and functional enrichment tests predict a potential function of DE-lncRNAs. Gene ontology classification and pathway analysis revealed 8 DE-lncRNAs, with the majority of their target genes enriched in biological functions such as reproductive structure development, developmental process involved in reproduction, response to cytokine, carbohydrate binding, chromatin organization, and immune pathways. Differential expression of lncRNAs and target genes were confirmed by qPCR. Together, these results significantly expand the utility of the UVJ transcriptome and our analysis identification of key lncRNAs and their target genes regulating DF will form the baseline for understanding the molecular functions of lncRNAs regulating DF.
format	article
author	Adeyinka Abiola Adetula Lantao Gu Chinedu Charles Nwafor Xiaoyong Du Shuhong Zhao Shijun Li
author_facet	Adeyinka Abiola Adetula Lantao Gu Chinedu Charles Nwafor Xiaoyong Du Shuhong Zhao Shijun Li
author_sort	Adeyinka Abiola Adetula
title	Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
title_short	Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
title_full	Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
title_fullStr	Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
title_full_unstemmed	Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens
title_sort	transcriptome sequencing reveals key potential long non-coding rnas related to duration of fertility trait in the uterovaginal junction of egg-laying hens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/93816f0c019f4d6a8ecd397bf242d597
work_keys_str_mv	AT adeyinkaabiolaadetula transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens AT lantaogu transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens AT chineducharlesnwafor transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens AT xiaoyongdu transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens AT shuhongzhao transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens AT shijunli transcriptomesequencingrevealskeypotentiallongnoncodingrnasrelatedtodurationoffertilitytraitintheuterovaginaljunctionofegglayinghens
_version_	1718388074741760000

Transcriptome sequencing reveals key potential long non-coding RNAs related to duration of fertility trait in the uterovaginal junction of egg-laying hens

Ejemplares similares