Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis

Silkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transiti...

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Autores principales:	Meirong Zhang, Pingzhen Xu, Tao Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Bombyx mori</i> maternal mRNAs 20-hydroxyecdysone pupa wandering Science Q
Acceso en línea:	https://doaj.org/article/93874b1457934a648957a530caeaaa72
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spelling	oai:doaj.org-article:93874b1457934a648957a530caeaaa722021-11-25T17:59:10ZHemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis10.3390/insects121109692075-4450https://doaj.org/article/93874b1457934a648957a530caeaaa722021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/969https://doaj.org/toc/2075-4450Silkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transition from previtellogenesis to vitellogenesis in silkworm oogenesis. In this study, we first investigated the expression patterns of 66 maternal genes in the ovary at the wandering stage. We then examined the developmental expression profiles in six time-series samples of ovaries or ovarioles by reverse transcription–quantitative PCR. We found that the transcripts of 22 maternal genes were regulated by 20-hydroxyecdysone in the isolated abdomens of the pupae following a single injection of 20-hydroxyecdysone. This study is the first to determine the relationship between 20-hydroxyecdysone and maternal genes during silkworm oogenesis. These findings provide a basis for further research into the embryonic development of <i>Bombyx mori</i>.Meirong ZhangPingzhen XuTao ChenMDPI AGarticle<i>Bombyx mori</i>maternal mRNAs20-hydroxyecdysonepupawanderingScienceQENInsects, Vol 12, Iss 969, p 969 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Bombyx mori</i> maternal mRNAs 20-hydroxyecdysone pupa wandering Science Q
spellingShingle	<i>Bombyx mori</i> maternal mRNAs 20-hydroxyecdysone pupa wandering Science Q Meirong Zhang Pingzhen Xu Tao Chen Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
description	Silkworm larval–pupal metamorphosis and the first half of pupal–adult development occur during oogenesis from previtellogenesis to vitellogenesis and include two peaks of the hemolymph ecdysteroid titer. Moreover, a rise in 20-hydroxyecdysone titer in early pupae can trigger the first major transition from previtellogenesis to vitellogenesis in silkworm oogenesis. In this study, we first investigated the expression patterns of 66 maternal genes in the ovary at the wandering stage. We then examined the developmental expression profiles in six time-series samples of ovaries or ovarioles by reverse transcription–quantitative PCR. We found that the transcripts of 22 maternal genes were regulated by 20-hydroxyecdysone in the isolated abdomens of the pupae following a single injection of 20-hydroxyecdysone. This study is the first to determine the relationship between 20-hydroxyecdysone and maternal genes during silkworm oogenesis. These findings provide a basis for further research into the embryonic development of <i>Bombyx mori</i>.
format	article
author	Meirong Zhang Pingzhen Xu Tao Chen
author_facet	Meirong Zhang Pingzhen Xu Tao Chen
author_sort	Meirong Zhang
title	Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
title_short	Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
title_full	Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
title_fullStr	Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
title_full_unstemmed	Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis
title_sort	hemolymph ecdysteroid titer affects maternal mrnas during <i>bombyx mori</i> oogenesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/93874b1457934a648957a530caeaaa72
work_keys_str_mv	AT meirongzhang hemolymphecdysteroidtiteraffectsmaternalmrnasduringibombyxmoriioogenesis AT pingzhenxu hemolymphecdysteroidtiteraffectsmaternalmrnasduringibombyxmoriioogenesis AT taochen hemolymphecdysteroidtiteraffectsmaternalmrnasduringibombyxmoriioogenesis
_version_	1718411759963865088

Hemolymph Ecdysteroid Titer Affects Maternal mRNAs during <i>Bombyx mori</i> Oogenesis

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