$Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions$
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Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

Abstract Previous study has shown the antimicrobial activities of mucus protein extracted from Anabas testudineus. In this study, we are interested in characterizing the anticancer activity of the A. testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to ant...

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Autores principales:	Ahmed Abdul Kareem Najm, Ahmad Azfaralariff, Herryawan Ryadi Eziwar Dyari, Babul Airianah Othman, Muhammad Shahid, Nahid Khalili, Douglas Law, Sharifah Sakinah Syed Alwi, Shazrul Fazry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/938ce12333ed4f2d97d12168e3305a9f
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spelling	oai:doaj.org-article:938ce12333ed4f2d97d12168e3305a9f2021-12-05T12:12:23ZAnti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions10.1038/s41598-021-02007-62045-2322https://doaj.org/article/938ce12333ed4f2d97d12168e3305a9f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02007-6https://doaj.org/toc/2045-2322Abstract Previous study has shown the antimicrobial activities of mucus protein extracted from Anabas testudineus. In this study, we are interested in characterizing the anticancer activity of the A. testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC50 for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.Ahmed Abdul Kareem NajmAhmad AzfaralariffHerryawan Ryadi Eziwar DyariBabul Airianah OthmanMuhammad ShahidNahid KhaliliDouglas LawSharifah Sakinah Syed AlwiShazrul FazryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmed Abdul Kareem Najm Ahmad Azfaralariff Herryawan Ryadi Eziwar Dyari Babul Airianah Othman Muhammad Shahid Nahid Khalili Douglas Law Sharifah Sakinah Syed Alwi Shazrul Fazry Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
description	Abstract Previous study has shown the antimicrobial activities of mucus protein extracted from Anabas testudineus. In this study, we are interested in characterizing the anticancer activity of the A. testudineus antimicrobial peptides (AMPs). The mucus was extracted, fractioned, and subjected to antibacterial activity testing to confirm the fish's AMPs production. The cytotoxic activity of each fraction was also identified. Fraction 2 (F2), which shows toxicity against MCF7 and MDA-MB-231 were sent for peptide sequencing to identify the bioactive peptide. The two peptides were then synthetically produced and subjected to cytotoxic assay to prove their efficacy against cancer cell lines. The IC50 for AtMP1 against MCF7 and MDA-MB-231 were 8.25 ± 0.14 μg/ml and 9.35 ± 0.25 μg/ml respectively, while for AtMP2 it is 5.89 ± 0.14 μg/ml and 6.97 ± 0.24 μg/ml respectively. AtMP1 and AtMP2 treatment for 48 h induced breast cancer cell cycle arrest and apoptosis by upregulating the p53, which lead to upregulate pro-apoptotic BAX gene and downregulate the anti-apoptotic BCL-2 gene, consequently, trigger the activation of the caspase-3. This interaction was supported by docking analysis (QuickDBD, HPEPDOCK, and ZDOCK) and immunoprecipitation. This study provided new prospects in the development of highly effective and selective cancer therapeutics based on antimicrobial peptides.
format	article
author	Ahmed Abdul Kareem Najm Ahmad Azfaralariff Herryawan Ryadi Eziwar Dyari Babul Airianah Othman Muhammad Shahid Nahid Khalili Douglas Law Sharifah Sakinah Syed Alwi Shazrul Fazry
author_facet	Ahmed Abdul Kareem Najm Ahmad Azfaralariff Herryawan Ryadi Eziwar Dyari Babul Airianah Othman Muhammad Shahid Nahid Khalili Douglas Law Sharifah Sakinah Syed Alwi Shazrul Fazry
author_sort	Ahmed Abdul Kareem Najm
title	Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
title_short	Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
title_full	Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
title_fullStr	Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
title_full_unstemmed	Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions
title_sort	anti-breast cancer synthetic peptides derived from the anabas testudineus skin mucus fractions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/938ce12333ed4f2d97d12168e3305a9f
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Anti-breast cancer synthetic peptides derived from the Anabas testudineus skin mucus fractions

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