Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio

The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated wi...

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Autores principales:	Ahmed Abdel-Wahab, Kamel M. A. Hassanin, Ahmed A. Mahmoud, Walaa I. E. Abdel-Badeea, Abdel-Razik H. Abdel-Razik, Eman Zekry Attia, Usama Ramadan Abdelmohsen, Rabie L. Abdel Aziz, Agnieszka Najda, Ibtesam S. Alanazi, Khalaf F. Alsharif, Mohamed M. Abdel-Daim, Mohamed O. Mahmoud
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red carrot methanolic extract cadmium chloride Vitamin E oxidative stress Bax/Bcl-2 ratio Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/9397d469e4934a668d734871aea7ffbe
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spelling	oai:doaj.org-article:9397d469e4934a668d734871aea7ffbe2021-11-25T16:25:23ZPhysiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio10.3390/antiox101116532076-3921https://doaj.org/article/9397d469e4934a668d734871aea7ffbe2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1653https://doaj.org/toc/2076-3921The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl<sub>2</sub>) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (<i>n</i> = 12) are used; control, CdCl<sub>2</sub>, CdCl<sub>2</sub> + Vit. E and CdCl<sub>2</sub> + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl<sub>2</sub> on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl<sub>2</sub> significantly succeeded in the modulation (<i>p</i> < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl<sub>2</sub> insult.Ahmed Abdel-WahabKamel M. A. HassaninAhmed A. MahmoudWalaa I. E. Abdel-BadeeaAbdel-Razik H. Abdel-RazikEman Zekry AttiaUsama Ramadan AbdelmohsenRabie L. Abdel AzizAgnieszka NajdaIbtesam S. AlanaziKhalaf F. AlsharifMohamed M. Abdel-DaimMohamed O. MahmoudMDPI AGarticlered carrot methanolic extractcadmium chlorideVitamin Eoxidative stressBax/Bcl-2 ratioTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1653, p 1653 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red carrot methanolic extract cadmium chloride Vitamin E oxidative stress Bax/Bcl-2 ratio Therapeutics. Pharmacology RM1-950
spellingShingle	red carrot methanolic extract cadmium chloride Vitamin E oxidative stress Bax/Bcl-2 ratio Therapeutics. Pharmacology RM1-950 Ahmed Abdel-Wahab Kamel M. A. Hassanin Ahmed A. Mahmoud Walaa I. E. Abdel-Badeea Abdel-Razik H. Abdel-Razik Eman Zekry Attia Usama Ramadan Abdelmohsen Rabie L. Abdel Aziz Agnieszka Najda Ibtesam S. Alanazi Khalaf F. Alsharif Mohamed M. Abdel-Daim Mohamed O. Mahmoud Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
description	The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl<sub>2</sub>) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (<i>n</i> = 12) are used; control, CdCl<sub>2</sub>, CdCl<sub>2</sub> + Vit. E and CdCl<sub>2</sub> + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl<sub>2</sub> on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl<sub>2</sub> significantly succeeded in the modulation (<i>p</i> < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl<sub>2</sub> insult.
format	article
author	Ahmed Abdel-Wahab Kamel M. A. Hassanin Ahmed A. Mahmoud Walaa I. E. Abdel-Badeea Abdel-Razik H. Abdel-Razik Eman Zekry Attia Usama Ramadan Abdelmohsen Rabie L. Abdel Aziz Agnieszka Najda Ibtesam S. Alanazi Khalaf F. Alsharif Mohamed M. Abdel-Daim Mohamed O. Mahmoud
author_facet	Ahmed Abdel-Wahab Kamel M. A. Hassanin Ahmed A. Mahmoud Walaa I. E. Abdel-Badeea Abdel-Razik H. Abdel-Razik Eman Zekry Attia Usama Ramadan Abdelmohsen Rabie L. Abdel Aziz Agnieszka Najda Ibtesam S. Alanazi Khalaf F. Alsharif Mohamed M. Abdel-Daim Mohamed O. Mahmoud
author_sort	Ahmed Abdel-Wahab
title	Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
title_short	Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
title_full	Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
title_fullStr	Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
title_full_unstemmed	Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio
title_sort	physiological roles of red carrot methanolic extract and vitamin e to abrogate cadmium-induced oxidative challenge and apoptosis in rat testes: involvement of the bax/bcl-2 ratio
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9397d469e4934a668d734871aea7ffbe
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Physiological Roles of Red Carrot Methanolic Extract and Vitamin E to Abrogate Cadmium-Induced Oxidative Challenge and Apoptosis in Rat Testes: Involvement of the Bax/Bcl-2 Ratio

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