Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA <i>miR-202-5p</i>, which regulates PGC migration through targeting <i>...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/93981d61f5214d80a9d3b77c3758abc6 |
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| Sumario: | MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA <i>miR-202-5p</i>, which regulates PGC migration through targeting <i>cdc42se1</i> to protect <i>cdc42</i> expression. However, knockdown of <i>cdc42se1</i> could not significantly rescue PGC migration in maternal <i>miR-202</i> mutant (M<i>miR-202</i>) embryos, indicating that there are other target genes of <i>miR-202-5p</i> required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and M<i>miR-202</i> PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel <i>miR-202-5p</i> target genes <i>prdm12b</i> and <i>rab10</i>. Furthermore, we found that disruption of <i>rab10</i> expression led to significantly migratory defects of PGC by overexpression of <i>rab10</i> siRNA, or WT, inactive as well as active forms of <i>rab10</i> mRNA, and WT <i>rab10</i> overexpression mediated migratory defects could be partially but significantly rescued by overexpression of <i>miR-202-5p</i>, demonstrating that <i>rab10</i> is an important factor involved <i>miR-202-5p</i> mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which <i>miR-202-5p</i> regulates PGC migration in zebrafish. |
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