Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish

MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA miR-202-5p, which regulates PGC migration through targeting ...

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Autores principales:	Chengyu Mo, Wenjing Li, Kuntong Jia, Wei Liu, Meisheng Yi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	primordial germ cells <i>miR-202-5p</i> <i>rab10</i> migration Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/93981d61f5214d80a9d3b77c3758abc6
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spelling	oai:doaj.org-article:93981d61f5214d80a9d3b77c3758abc62021-11-11T17:22:56ZProper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish10.3390/ijms2221119621422-00671661-6596https://doaj.org/article/93981d61f5214d80a9d3b77c3758abc62021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11962https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA <i>miR-202-5p</i>, which regulates PGC migration through targeting <i>cdc42se1</i> to protect <i>cdc42</i> expression. However, knockdown of <i>cdc42se1</i> could not significantly rescue PGC migration in maternal <i>miR-202</i> mutant (M<i>miR-202</i>) embryos, indicating that there are other target genes of <i>miR-202-5p</i> required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and M<i>miR-202</i> PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel <i>miR-202-5p</i> target genes <i>prdm12b</i> and <i>rab10</i>. Furthermore, we found that disruption of <i>rab10</i> expression led to significantly migratory defects of PGC by overexpression of <i>rab10</i> siRNA, or WT, inactive as well as active forms of <i>rab10</i> mRNA, and WT <i>rab10</i> overexpression mediated migratory defects could be partially but significantly rescued by overexpression of <i>miR-202-5p</i>, demonstrating that <i>rab10</i> is an important factor involved <i>miR-202-5p</i> mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which <i>miR-202-5p</i> regulates PGC migration in zebrafish.Chengyu MoWenjing LiKuntong JiaWei LiuMeisheng YiMDPI AGarticleprimordial germ cells<i>miR-202-5p</i><i>rab10</i>migrationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11962, p 11962 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	primordial germ cells <i>miR-202-5p</i> <i>rab10</i> migration Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	primordial germ cells <i>miR-202-5p</i> <i>rab10</i> migration Biology (General) QH301-705.5 Chemistry QD1-999 Chengyu Mo Wenjing Li Kuntong Jia Wei Liu Meisheng Yi Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
description	MicroRNAs (miRNAs) play important roles in post-transcriptional repression in nearly every biological process including germ cell development. Previously, we have identified a zebrafish germ plasm-specific miRNA <i>miR-202-5p</i>, which regulates PGC migration through targeting <i>cdc42se1</i> to protect <i>cdc42</i> expression. However, knockdown of <i>cdc42se1</i> could not significantly rescue PGC migration in maternal <i>miR-202</i> mutant (M<i>miR-202</i>) embryos, indicating that there are other target genes of <i>miR-202-5p</i> required for the regulation of PGC migration. Herein, we revealed the transcriptional profiles of wild type and M<i>miR-202</i> PGCs and obtained 129 differentially expressed genes (DEGs), of which 42 DEGs were enriched cell migration-related signaling pathways. From these DEGs, we identified two novel <i>miR-202-5p</i> target genes <i>prdm12b</i> and <i>rab10</i>. Furthermore, we found that disruption of <i>rab10</i> expression led to significantly migratory defects of PGC by overexpression of <i>rab10</i> siRNA, or WT, inactive as well as active forms of <i>rab10</i> mRNA, and WT <i>rab10</i> overexpression mediated migratory defects could be partially but significantly rescued by overexpression of <i>miR-202-5p</i>, demonstrating that <i>rab10</i> is an important factor involved <i>miR-202-5p</i> mediated regulation of PGC migration. Taken together, the present results provide significant information for understanding the molecular mechanism by which <i>miR-202-5p</i> regulates PGC migration in zebrafish.
format	article
author	Chengyu Mo Wenjing Li Kuntong Jia Wei Liu Meisheng Yi
author_facet	Chengyu Mo Wenjing Li Kuntong Jia Wei Liu Meisheng Yi
author_sort	Chengyu Mo
title	Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
title_short	Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
title_full	Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
title_fullStr	Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
title_full_unstemmed	Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish
title_sort	proper balance of small gtpase <i>rab10</i> is critical for pgc migration in zebrafish
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/93981d61f5214d80a9d3b77c3758abc6
work_keys_str_mv	AT chengyumo properbalanceofsmallgtpaseirab10iiscriticalforpgcmigrationinzebrafish AT wenjingli properbalanceofsmallgtpaseirab10iiscriticalforpgcmigrationinzebrafish AT kuntongjia properbalanceofsmallgtpaseirab10iiscriticalforpgcmigrationinzebrafish AT weiliu properbalanceofsmallgtpaseirab10iiscriticalforpgcmigrationinzebrafish AT meishengyi properbalanceofsmallgtpaseirab10iiscriticalforpgcmigrationinzebrafish
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Proper Balance of Small GTPase <i>rab10</i> Is Critical for PGC Migration in Zebrafish

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