A Japanese prospective multicenter study of urinary oxysterols in biliary atresia
Abstract Diagnosis of biliary atresia (BA) can involve uncertainties. In the present prospective multicenter study, we considered whether urinary oxysterols represent a useful marker for diagnosis of BA in Japanese children. Subjects under 6 months old at 7 pediatric centers in Japan were prospectiv...
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Nature Portfolio
2021
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oai:doaj.org-article:939c855337f941dcb4b91194b34430a72021-12-02T11:35:59ZA Japanese prospective multicenter study of urinary oxysterols in biliary atresia10.1038/s41598-021-84445-w2045-2322https://doaj.org/article/939c855337f941dcb4b91194b34430a72021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84445-whttps://doaj.org/toc/2045-2322Abstract Diagnosis of biliary atresia (BA) can involve uncertainties. In the present prospective multicenter study, we considered whether urinary oxysterols represent a useful marker for diagnosis of BA in Japanese children. Subjects under 6 months old at 7 pediatric centers in Japan were prospectively enrolled, including patients with cholestasis and healthy controls (HC) without liver disease. Patients with cholestasis constituted 2 groups representing BA patients and others with cholestasis from other causes (non-BA). We quantitatively analyzed 7 oxysterols including 4β-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol by liquid chromatography/electrospray ionization-tandem mass spectrometry. Enrolled subjects included 14 with BA (median age 68 days; range 26–170) and 10 non-BA cholestatic controls (59; 14–162), as well as 10 HC (57; 25–120). Total urinary oxysterols were significantly greater in BA (median, 153.0 μmol/mol creatinine; range 24.1–486.7; P < 0.001) and non-BA (36.2; 5.8–411.3; P < 0.05) than in HC (2.7; 0.8–7.6). In patients with BA, urinary 27-hydroxycholesterol (3.61; 0.42–11.09; P < 0.01) was significantly greater than in non-BA (0.71; 0–5.62). In receiver operating characteristic (ROC) curve analysis for distinguishing BA from non-BA, the area under the ROC curve for urinary 27-hydroxycholesterol was 0.83. In conclusion, this first report of urinary oxysterol analysis in patients with BA indicated that 27-hydroxycholesterol may be a useful marker for distinguishing BA from other causes of neonatal cholestasis.Ken-ichiro KonishiTatsuki MizuochiHajime TakeiRyosuke YasudaHirotaka SakaguchiJun IshiharaYugo TakakiMasahiro KinoshitaNaoki HashizumeSuguru FukahoriHiromichi ShojiGo MiyanoKoichiro YoshimaruToshiharu MatsuuraYukihiro SanadaTakahisa TainakaHiroo UchidaYumiko KuboHiromu TanakaHideyuki SasakiTsuyoshi MuraiJun FujishiroYushiro YamashitaMasaki NioHiroshi NittonoAkihiko KimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Ken-ichiro Konishi Tatsuki Mizuochi Hajime Takei Ryosuke Yasuda Hirotaka Sakaguchi Jun Ishihara Yugo Takaki Masahiro Kinoshita Naoki Hashizume Suguru Fukahori Hiromichi Shoji Go Miyano Koichiro Yoshimaru Toshiharu Matsuura Yukihiro Sanada Takahisa Tainaka Hiroo Uchida Yumiko Kubo Hiromu Tanaka Hideyuki Sasaki Tsuyoshi Murai Jun Fujishiro Yushiro Yamashita Masaki Nio Hiroshi Nittono Akihiko Kimura A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| description |
Abstract Diagnosis of biliary atresia (BA) can involve uncertainties. In the present prospective multicenter study, we considered whether urinary oxysterols represent a useful marker for diagnosis of BA in Japanese children. Subjects under 6 months old at 7 pediatric centers in Japan were prospectively enrolled, including patients with cholestasis and healthy controls (HC) without liver disease. Patients with cholestasis constituted 2 groups representing BA patients and others with cholestasis from other causes (non-BA). We quantitatively analyzed 7 oxysterols including 4β-, 20(S)-, 22(S)-, 22(R)-, 24(S)-, 25-, and 27-hydroxycholesterol by liquid chromatography/electrospray ionization-tandem mass spectrometry. Enrolled subjects included 14 with BA (median age 68 days; range 26–170) and 10 non-BA cholestatic controls (59; 14–162), as well as 10 HC (57; 25–120). Total urinary oxysterols were significantly greater in BA (median, 153.0 μmol/mol creatinine; range 24.1–486.7; P < 0.001) and non-BA (36.2; 5.8–411.3; P < 0.05) than in HC (2.7; 0.8–7.6). In patients with BA, urinary 27-hydroxycholesterol (3.61; 0.42–11.09; P < 0.01) was significantly greater than in non-BA (0.71; 0–5.62). In receiver operating characteristic (ROC) curve analysis for distinguishing BA from non-BA, the area under the ROC curve for urinary 27-hydroxycholesterol was 0.83. In conclusion, this first report of urinary oxysterol analysis in patients with BA indicated that 27-hydroxycholesterol may be a useful marker for distinguishing BA from other causes of neonatal cholestasis. |
| format |
article |
| author |
Ken-ichiro Konishi Tatsuki Mizuochi Hajime Takei Ryosuke Yasuda Hirotaka Sakaguchi Jun Ishihara Yugo Takaki Masahiro Kinoshita Naoki Hashizume Suguru Fukahori Hiromichi Shoji Go Miyano Koichiro Yoshimaru Toshiharu Matsuura Yukihiro Sanada Takahisa Tainaka Hiroo Uchida Yumiko Kubo Hiromu Tanaka Hideyuki Sasaki Tsuyoshi Murai Jun Fujishiro Yushiro Yamashita Masaki Nio Hiroshi Nittono Akihiko Kimura |
| author_facet |
Ken-ichiro Konishi Tatsuki Mizuochi Hajime Takei Ryosuke Yasuda Hirotaka Sakaguchi Jun Ishihara Yugo Takaki Masahiro Kinoshita Naoki Hashizume Suguru Fukahori Hiromichi Shoji Go Miyano Koichiro Yoshimaru Toshiharu Matsuura Yukihiro Sanada Takahisa Tainaka Hiroo Uchida Yumiko Kubo Hiromu Tanaka Hideyuki Sasaki Tsuyoshi Murai Jun Fujishiro Yushiro Yamashita Masaki Nio Hiroshi Nittono Akihiko Kimura |
| author_sort |
Ken-ichiro Konishi |
| title |
A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| title_short |
A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| title_full |
A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| title_fullStr |
A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| title_full_unstemmed |
A Japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| title_sort |
japanese prospective multicenter study of urinary oxysterols in biliary atresia |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/939c855337f941dcb4b91194b34430a7 |
| work_keys_str_mv |
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