MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR

Context: Evidence suggests that microRNA (miRNA) regulates gene expression and bone tissue homoeostasis of osteoporosis. MiR-152 has found to be abnormally expressed in osteoporosis, but its role in osteoblast differentiation has not been elucidated. Objective: To understand the potential mechanism...

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Autores principales:	Li Feng, Bo Xia, Bao-Fang Tian, Gong-Biao Lu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	microrna ovariectomized rat primary osteoblasts mc3t3-e1 cells Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/939e238c31bd43fdb5a34b7bfe6c09e7
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spelling	oai:doaj.org-article:939e238c31bd43fdb5a34b7bfe6c09e72021-11-17T14:21:56ZMiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR1388-02091744-511610.1080/13880209.2019.1657153https://doaj.org/article/939e238c31bd43fdb5a34b7bfe6c09e72019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1657153https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Evidence suggests that microRNA (miRNA) regulates gene expression and bone tissue homoeostasis of osteoporosis. MiR-152 has found to be abnormally expressed in osteoporosis, but its role in osteoblast differentiation has not been elucidated. Objective: To understand the potential mechanism of miR-152 in osteoblast differentiation via regulation of RICTOR. Materials and methods: The expression of miR-152 and RICTOR were tested in ovariectomized rat models of osteoporosis. Primary osteoblasts and MC3T -E1 cells were assigned into four groups, namely Control, miR-152 inhibitor, miR-control and miR-152 inhibitor + siRICTOR groups. qRT PCR and Western blots were performed to detect the expression of miR-152 and RICTOR, respectively. MTT assay was used to evaluate cell viability, and ALP activity determination and mineralization analyses were also conducted. Results: In ovariectomy-induced osteoporotic rats, miR-152 (3.06 ± 0.35) in femoral tissues increased significantly, while RICTOR (0.31 ± 0.04) decreased. Compared with the Control group, the miR-152 inhibitor group presented appreciable reduction of miR-152 in primary osteoblasts and MC3T3-E1 cells, as well as remarkable increases in RICTOR, p-Akt(s473)/Akt ratio, and osteogenesis-related genes, with enhanced cell viability, ALP activity and mineralization. In comparison with cells in the miR-152 inhibitor group, those in the miR-152 inhibitor + siRICTOR group had no observable difference in miR-152, but were dramatically up-regulated in RICTOR, as well as the corresponding opposite tendencies of other factors. Conclusion: Inhibiting miR-152 promoted osteoblasts differentiation and alleviated osteoporosis by up-regulating RICTOR. Therefore, miR-152 may be an essential mediator of osteoblast differentiation and a new therapeutic strategy for osteoporosis.Li FengBo XiaBao-Fang TianGong-Biao LuTaylor & Francis Grouparticlemicrornaovariectomized ratprimary osteoblastsmc3t3-e1 cellsTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 586-594 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna ovariectomized rat primary osteoblasts mc3t3-e1 cells Therapeutics. Pharmacology RM1-950
spellingShingle	microrna ovariectomized rat primary osteoblasts mc3t3-e1 cells Therapeutics. Pharmacology RM1-950 Li Feng Bo Xia Bao-Fang Tian Gong-Biao Lu MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
description	Context: Evidence suggests that microRNA (miRNA) regulates gene expression and bone tissue homoeostasis of osteoporosis. MiR-152 has found to be abnormally expressed in osteoporosis, but its role in osteoblast differentiation has not been elucidated. Objective: To understand the potential mechanism of miR-152 in osteoblast differentiation via regulation of RICTOR. Materials and methods: The expression of miR-152 and RICTOR were tested in ovariectomized rat models of osteoporosis. Primary osteoblasts and MC3T -E1 cells were assigned into four groups, namely Control, miR-152 inhibitor, miR-control and miR-152 inhibitor + siRICTOR groups. qRT PCR and Western blots were performed to detect the expression of miR-152 and RICTOR, respectively. MTT assay was used to evaluate cell viability, and ALP activity determination and mineralization analyses were also conducted. Results: In ovariectomy-induced osteoporotic rats, miR-152 (3.06 ± 0.35) in femoral tissues increased significantly, while RICTOR (0.31 ± 0.04) decreased. Compared with the Control group, the miR-152 inhibitor group presented appreciable reduction of miR-152 in primary osteoblasts and MC3T3-E1 cells, as well as remarkable increases in RICTOR, p-Akt(s473)/Akt ratio, and osteogenesis-related genes, with enhanced cell viability, ALP activity and mineralization. In comparison with cells in the miR-152 inhibitor group, those in the miR-152 inhibitor + siRICTOR group had no observable difference in miR-152, but were dramatically up-regulated in RICTOR, as well as the corresponding opposite tendencies of other factors. Conclusion: Inhibiting miR-152 promoted osteoblasts differentiation and alleviated osteoporosis by up-regulating RICTOR. Therefore, miR-152 may be an essential mediator of osteoblast differentiation and a new therapeutic strategy for osteoporosis.
format	article
author	Li Feng Bo Xia Bao-Fang Tian Gong-Biao Lu
author_facet	Li Feng Bo Xia Bao-Fang Tian Gong-Biao Lu
author_sort	Li Feng
title	MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
title_short	MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
title_full	MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
title_fullStr	MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
title_full_unstemmed	MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR
title_sort	mir-152 influences osteoporosis through regulation of osteoblast differentiation by targeting rictor
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/939e238c31bd43fdb5a34b7bfe6c09e7
work_keys_str_mv	AT lifeng mir152influencesosteoporosisthroughregulationofosteoblastdifferentiationbytargetingrictor AT boxia mir152influencesosteoporosisthroughregulationofosteoblastdifferentiationbytargetingrictor AT baofangtian mir152influencesosteoporosisthroughregulationofosteoblastdifferentiationbytargetingrictor AT gongbiaolu mir152influencesosteoporosisthroughregulationofosteoblastdifferentiationbytargetingrictor
_version_	1718425496297930752

MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR

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