Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response

Abstract Ultrasound (US) neuromodulation, especially sonogenetics, has been demonstrated with potential applications in noninvasive and targeted treatment of various neurological disorders. Despite the growing interest, the mechanism for US neuromodulation remains elusive, and the optimal condition...

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Autores principales:	Defei Liao, Ming-Yen Hsiao, Gaoming Xiang, Pei Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93a6812375684ee7ab1e33650c2f980a
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spelling	oai:doaj.org-article:93a6812375684ee7ab1e33650c2f980a2021-12-02T15:23:07ZOptimal pulse length of insonification for Piezo1 activation and intracellular calcium response10.1038/s41598-020-78553-22045-2322https://doaj.org/article/93a6812375684ee7ab1e33650c2f980a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78553-2https://doaj.org/toc/2045-2322Abstract Ultrasound (US) neuromodulation, especially sonogenetics, has been demonstrated with potential applications in noninvasive and targeted treatment of various neurological disorders. Despite the growing interest, the mechanism for US neuromodulation remains elusive, and the optimal condition for eliciting a neural response with minimal adverse effect has not been identified. Here, we investigate the Piezo1 activation and intracellular calcium response elicited by acoustical streaming induced shear stress under various US exposure conditions. We find that Piezo1 activation and resultant intracellular calcium response depend critically on shear stress amplitude and pulse length of the stimulation. Under the same insonification acoustic energy, we further identify an optical pulse length that leads to maximum cell deformation, Piezo1 activation, and calcium response with minimal injury, confirmed by numerical modeling of Piezo1 channel gating dynamics. Our results provide insight into the mechanism of ultrasonic activation of Piezo1 and highlight the importance of optimizing US exposure conditions in sonogenetics applications.Defei LiaoMing-Yen HsiaoGaoming XiangPei ZhongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Defei Liao Ming-Yen Hsiao Gaoming Xiang Pei Zhong Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
description	Abstract Ultrasound (US) neuromodulation, especially sonogenetics, has been demonstrated with potential applications in noninvasive and targeted treatment of various neurological disorders. Despite the growing interest, the mechanism for US neuromodulation remains elusive, and the optimal condition for eliciting a neural response with minimal adverse effect has not been identified. Here, we investigate the Piezo1 activation and intracellular calcium response elicited by acoustical streaming induced shear stress under various US exposure conditions. We find that Piezo1 activation and resultant intracellular calcium response depend critically on shear stress amplitude and pulse length of the stimulation. Under the same insonification acoustic energy, we further identify an optical pulse length that leads to maximum cell deformation, Piezo1 activation, and calcium response with minimal injury, confirmed by numerical modeling of Piezo1 channel gating dynamics. Our results provide insight into the mechanism of ultrasonic activation of Piezo1 and highlight the importance of optimizing US exposure conditions in sonogenetics applications.
format	article
author	Defei Liao Ming-Yen Hsiao Gaoming Xiang Pei Zhong
author_facet	Defei Liao Ming-Yen Hsiao Gaoming Xiang Pei Zhong
author_sort	Defei Liao
title	Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
title_short	Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
title_full	Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
title_fullStr	Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
title_full_unstemmed	Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response
title_sort	optimal pulse length of insonification for piezo1 activation and intracellular calcium response
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/93a6812375684ee7ab1e33650c2f980a
work_keys_str_mv	AT defeiliao optimalpulselengthofinsonificationforpiezo1activationandintracellularcalciumresponse AT mingyenhsiao optimalpulselengthofinsonificationforpiezo1activationandintracellularcalciumresponse AT gaomingxiang optimalpulselengthofinsonificationforpiezo1activationandintracellularcalciumresponse AT peizhong optimalpulselengthofinsonificationforpiezo1activationandintracellularcalciumresponse
_version_	1718387339209736192

Optimal pulse length of insonification for Piezo1 activation and intracellular calcium response

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