Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.

3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the tw...

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Autores principales:	Kristoffer Søberg, Tore Jahnsen, Torbjørn Rognes, Bjørn S Skålhegg, Jon K Laerdahl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93ae8b053e6f4790a66e27c91ff52310
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spelling	oai:doaj.org-article:93ae8b053e6f4790a66e27c91ff523102021-11-18T07:49:37ZEvolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.1932-620310.1371/journal.pone.0060935https://doaj.org/article/93ae8b053e6f4790a66e27c91ff523102013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593352/pdf/?tool=EBIhttps://doaj.org/toc/1932-62033',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the two genes PRKACA and PRKACB, respectively, are among the best understood and characterized human kinases. Here we have studied the evolution of this gene family in chordates, arthropods, mollusks and other animals employing probabilistic methods and show that Cα and Cβ arose by duplication of an ancestral PKA catalytic subunit in a common ancestor of vertebrates. The two genes have subsequently been duplicated in teleost fishes. The evolution of the PRKACG retroposon in simians was also investigated. Although the degree of sequence conservation in the PKA Cα/Cβ kinase family is exceptionally high, a small set of signature residues defining Cα and Cβ subfamilies were identified. These conserved residues might be important for functions that are unique to the Cα or Cβ clades. This study also provides a good example of a seemingly simple phylogenetic problem which, due to a very high degree of sequence conservation and corresponding weak phylogenetic signals, combined with problematic nonphylogenetic signals, is nontrivial for state-of-the-art probabilistic phylogenetic methods.Kristoffer SøbergTore JahnsenTorbjørn RognesBjørn S SkålheggJon K LaerdahlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60935 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
description	3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the two genes PRKACA and PRKACB, respectively, are among the best understood and characterized human kinases. Here we have studied the evolution of this gene family in chordates, arthropods, mollusks and other animals employing probabilistic methods and show that Cα and Cβ arose by duplication of an ancestral PKA catalytic subunit in a common ancestor of vertebrates. The two genes have subsequently been duplicated in teleost fishes. The evolution of the PRKACG retroposon in simians was also investigated. Although the degree of sequence conservation in the PKA Cα/Cβ kinase family is exceptionally high, a small set of signature residues defining Cα and Cβ subfamilies were identified. These conserved residues might be important for functions that are unique to the Cα or Cβ clades. This study also provides a good example of a seemingly simple phylogenetic problem which, due to a very high degree of sequence conservation and corresponding weak phylogenetic signals, combined with problematic nonphylogenetic signals, is nontrivial for state-of-the-art probabilistic phylogenetic methods.
format	article
author	Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl
author_facet	Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl
author_sort	Kristoffer Søberg
title	Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
title_short	Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
title_full	Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
title_fullStr	Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
title_full_unstemmed	Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
title_sort	evolutionary paths of the camp-dependent protein kinase (pka) catalytic subunits.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/93ae8b053e6f4790a66e27c91ff52310
work_keys_str_mv	AT kristoffersøberg evolutionarypathsofthecampdependentproteinkinasepkacatalyticsubunits AT torejahnsen evolutionarypathsofthecampdependentproteinkinasepkacatalyticsubunits AT torbjørnrognes evolutionarypathsofthecampdependentproteinkinasepkacatalyticsubunits AT bjørnsskalhegg evolutionarypathsofthecampdependentproteinkinasepkacatalyticsubunits AT jonklaerdahl evolutionarypathsofthecampdependentproteinkinasepkacatalyticsubunits
_version_	1718422925091012608

Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.

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