Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes

Junyan Wang,1 Jiahui Zhou,2 Shuancheng Bai2 1Department of Critical Care Medicine, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of China; 2Department of Anesthesiology, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of ChinaCorrespondence...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang J, Zhou J, Bai S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	sepsis glutamine ulinastatin inflammation immune function Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/93bb57d20d0443dab9eb3c90219504c1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:93bb57d20d0443dab9eb3c90219504c1
record_format	dspace
spelling	oai:doaj.org-article:93bb57d20d0443dab9eb3c90219504c12021-12-02T05:35:36ZCombination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes1178-7031https://doaj.org/article/93bb57d20d0443dab9eb3c90219504c12020-02-01T00:00:00Zhttps://www.dovepress.com/combination-of-glutamine-and-ulinastatin-treatments-greatly-improves-s-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Junyan Wang,1 Jiahui Zhou,2 Shuancheng Bai2 1Department of Critical Care Medicine, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of China; 2Department of Anesthesiology, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of ChinaCorrespondence: Shuancheng Bai Email bscwjy@sina.comBackground: Sepsis is one of the most dangerous syndromes, has extremely high mortality, and is caused by the body’s extreme responses to an infection. The pathogenesis of sepsis is very complex and remains largely unknown and thus the treatments for sepsis are limited. Here, we evaluated the treatment results of two potential drugs, glutamine and ulinastatin, on sepsis.Methods: CLP rat model was used to study sepsis. Gastrostomy was performed to deliver the drugs. Flow cytometry was employed to measure CD4 and CD8 levels. May–Grünwald–Giemsa staining was used to count the numbers of monocytes and neutrophils in the blood. ELISA assay was performed to assess the levels of PCT, IL-6, TNFα, and IL-1β.Results: Sepsis was successfully induced with the standard CLP rat model. Both glutamine and ulinastatin treatments greatly improved the outcomes of sepsis, but the combination of both treatments had the maximum therapeutic effect. Mechanistically, PCT, IL-6, TNFα, and IL-1β levels were significantly diminished following glutamine and ulinastatin treatments, suggesting an inhibition of inflammatory responses. Further, CD4 and CD4/CD8 ratio, and the numbers of monocytes and neutrophils were greatly up-regulated by glutamine and ulinastatin, indicating an enhanced immunity.Conclusion: Glutamine and ulinastatin treatments largely mitigate sepsis shock by suppressing the inflammatory responses of the body and strengthening the immune system. Combination of these two drugs could serve as a potential treatment for sepsis.Keywords: sepsis, glutamine, ulinastatin, inflammation, immune functionWang JZhou JBai SDove Medical Pressarticlesepsisglutamineulinastatininflammationimmune functionPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 109-115 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	sepsis glutamine ulinastatin inflammation immune function Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	sepsis glutamine ulinastatin inflammation immune function Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Wang J Zhou J Bai S Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
description	Junyan Wang,1 Jiahui Zhou,2 Shuancheng Bai2 1Department of Critical Care Medicine, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of China; 2Department of Anesthesiology, Baotou Central Hospital, Baotou, Inner Mongolia, People’s Republic of ChinaCorrespondence: Shuancheng Bai Email bscwjy@sina.comBackground: Sepsis is one of the most dangerous syndromes, has extremely high mortality, and is caused by the body’s extreme responses to an infection. The pathogenesis of sepsis is very complex and remains largely unknown and thus the treatments for sepsis are limited. Here, we evaluated the treatment results of two potential drugs, glutamine and ulinastatin, on sepsis.Methods: CLP rat model was used to study sepsis. Gastrostomy was performed to deliver the drugs. Flow cytometry was employed to measure CD4 and CD8 levels. May–Grünwald–Giemsa staining was used to count the numbers of monocytes and neutrophils in the blood. ELISA assay was performed to assess the levels of PCT, IL-6, TNFα, and IL-1β.Results: Sepsis was successfully induced with the standard CLP rat model. Both glutamine and ulinastatin treatments greatly improved the outcomes of sepsis, but the combination of both treatments had the maximum therapeutic effect. Mechanistically, PCT, IL-6, TNFα, and IL-1β levels were significantly diminished following glutamine and ulinastatin treatments, suggesting an inhibition of inflammatory responses. Further, CD4 and CD4/CD8 ratio, and the numbers of monocytes and neutrophils were greatly up-regulated by glutamine and ulinastatin, indicating an enhanced immunity.Conclusion: Glutamine and ulinastatin treatments largely mitigate sepsis shock by suppressing the inflammatory responses of the body and strengthening the immune system. Combination of these two drugs could serve as a potential treatment for sepsis.Keywords: sepsis, glutamine, ulinastatin, inflammation, immune function
format	article
author	Wang J Zhou J Bai S
author_facet	Wang J Zhou J Bai S
author_sort	Wang J
title	Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
title_short	Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
title_full	Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
title_fullStr	Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
title_full_unstemmed	Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes
title_sort	combination of glutamine and ulinastatin treatments greatly improves sepsis outcomes
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/93bb57d20d0443dab9eb3c90219504c1
work_keys_str_mv	AT wangj combinationofglutamineandulinastatintreatmentsgreatlyimprovessepsisoutcomes AT zhouj combinationofglutamineandulinastatintreatmentsgreatlyimprovessepsisoutcomes AT bais combinationofglutamineandulinastatintreatmentsgreatlyimprovessepsisoutcomes
_version_	1718400364332449792

Combination of Glutamine and Ulinastatin Treatments Greatly Improves Sepsis Outcomes

Ejemplares similares