Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D

Abstract Collagen is widely used in tissue engineering and regenerative medicine, with many examples of collagen-based biomaterials emerging in recent years. While there are numerous methods available for forming collagen scaffolds from isolated collagen, existing biomaterial processing techniques a...

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Autores principales:	G. Y. Liu, R. Agarwal, K. R Ko, M. Ruthven, H. T. Sarhan, J. P. Frampton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93db865081a14bfebac399bab0b60f31
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spelling	oai:doaj.org-article:93db865081a14bfebac399bab0b60f312021-12-02T15:05:00ZTemplated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D10.1038/s41598-017-10182-82045-2322https://doaj.org/article/93db865081a14bfebac399bab0b60f312017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10182-8https://doaj.org/toc/2045-2322Abstract Collagen is widely used in tissue engineering and regenerative medicine, with many examples of collagen-based biomaterials emerging in recent years. While there are numerous methods available for forming collagen scaffolds from isolated collagen, existing biomaterial processing techniques are unable to efficiently align collagen at the microstructural level, which is important for providing appropriate cell recognition and mechanical properties. Although some attention has shifted to development of fiber-based collagen biomaterials, existing techniques for producing and aligning collagen fibers are not appropriate for large-scale fiber manufacturing. Here, we report a novel biomaterial fabrication approach capable of efficiently generating collagen fibers of appropriate sizes using a viscous solution of dextran as a dissolvable template. We demonstrate that myoblasts readily attach and align along 2D collagen fiber networks created by this process. Furthermore, encapsulation of collagen fibers with myoblasts into non-cell-adherent hydrogels promotes aligned growth of cells and supports their differentiation. The ease-of-production and versatility of this technique will support future development of advanced in vitro tissue models and materials for regenerative medicine.G. Y. LiuR. AgarwalK. R KoM. RuthvenH. T. SarhanJ. P. FramptonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q G. Y. Liu R. Agarwal K. R Ko M. Ruthven H. T. Sarhan J. P. Frampton Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
description	Abstract Collagen is widely used in tissue engineering and regenerative medicine, with many examples of collagen-based biomaterials emerging in recent years. While there are numerous methods available for forming collagen scaffolds from isolated collagen, existing biomaterial processing techniques are unable to efficiently align collagen at the microstructural level, which is important for providing appropriate cell recognition and mechanical properties. Although some attention has shifted to development of fiber-based collagen biomaterials, existing techniques for producing and aligning collagen fibers are not appropriate for large-scale fiber manufacturing. Here, we report a novel biomaterial fabrication approach capable of efficiently generating collagen fibers of appropriate sizes using a viscous solution of dextran as a dissolvable template. We demonstrate that myoblasts readily attach and align along 2D collagen fiber networks created by this process. Furthermore, encapsulation of collagen fibers with myoblasts into non-cell-adherent hydrogels promotes aligned growth of cells and supports their differentiation. The ease-of-production and versatility of this technique will support future development of advanced in vitro tissue models and materials for regenerative medicine.
format	article
author	G. Y. Liu R. Agarwal K. R Ko M. Ruthven H. T. Sarhan J. P. Frampton
author_facet	G. Y. Liu R. Agarwal K. R Ko M. Ruthven H. T. Sarhan J. P. Frampton
author_sort	G. Y. Liu
title	Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
title_short	Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
title_full	Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
title_fullStr	Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
title_full_unstemmed	Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D
title_sort	templated assembly of collagen fibers directs cell growth in 2d and 3d
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/93db865081a14bfebac399bab0b60f31
work_keys_str_mv	AT gyliu templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d AT ragarwal templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d AT krko templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d AT mruthven templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d AT htsarhan templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d AT jpframpton templatedassemblyofcollagenfibersdirectscellgrowthin2dand3d
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Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D

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