Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function

Iron supplementation is necessary for the treatment of anemia, one of the most frequent complications in inflammatory bowel disease (IBD). However, oral iron supplementation leads to an exacerbation of intestinal inflammation. Gut barrier plays a key role in the pathogenesis of IBD. The aim of this...

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Autores principales:	Li Liang, Qingqing Xiong, Jingxia Kong, Chenying Tian, Linfeng Miao, Xiaofeng Zhang, Huahua Du
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Iron supplementation Iron injection Colitis Intestinal barrier function Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/93e3fb2e471a41af9d7fad5eb33a0332
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spelling	oai:doaj.org-article:93e3fb2e471a41af9d7fad5eb33a03322021-11-14T04:28:52ZIntraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function0753-332210.1016/j.biopha.2021.112253https://doaj.org/article/93e3fb2e471a41af9d7fad5eb33a03322021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221010374https://doaj.org/toc/0753-3322Iron supplementation is necessary for the treatment of anemia, one of the most frequent complications in inflammatory bowel disease (IBD). However, oral iron supplementation leads to an exacerbation of intestinal inflammation. Gut barrier plays a key role in the pathogenesis of IBD. The aim of this study was to characterize the interrelationship between systemic iron, intestinal barrier and the development of intestinal inflammation in a dextran sulfate sodium (DSS) induced experimental colitis mice model. We found that DSS-treated mice developed severe inflammation of colon, but became much healthy when intraperitoneal injection with iron. Iron supplementation alleviated colonic and systemic inflammation by lower histological scores, restorative morphology of colonic villi, and reduced expression of pro-inflammatory cytokines. Moreover, intraperitoneal supplementation of iron enhanced intestinal barrier function by upregulating the colonic expressions of tight junction proteins, restoring intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and increasing mucous secretion of goblet cells in the colon. High-throughput sequencing of fecal 16 S rRNA showed that iron injection significantly increased the relative abundance of Bacteroidetes, which was suppressed in the gut microbiota of DSS-induced colitis mice. These results provided evidences supporting the protective effects of systemic iron repletion by intraperitoneal injection of iron on intestinal barrier functions. The finding highlights a novel approach for the treatment of IBD with iron injection therapy.Li LiangQingqing XiongJingxia KongChenying TianLinfeng MiaoXiaofeng ZhangHuahua DuElsevierarticleIron supplementationIron injectionColitisIntestinal barrier functionTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112253- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Iron supplementation Iron injection Colitis Intestinal barrier function Therapeutics. Pharmacology RM1-950
spellingShingle	Iron supplementation Iron injection Colitis Intestinal barrier function Therapeutics. Pharmacology RM1-950 Li Liang Qingqing Xiong Jingxia Kong Chenying Tian Linfeng Miao Xiaofeng Zhang Huahua Du Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
description	Iron supplementation is necessary for the treatment of anemia, one of the most frequent complications in inflammatory bowel disease (IBD). However, oral iron supplementation leads to an exacerbation of intestinal inflammation. Gut barrier plays a key role in the pathogenesis of IBD. The aim of this study was to characterize the interrelationship between systemic iron, intestinal barrier and the development of intestinal inflammation in a dextran sulfate sodium (DSS) induced experimental colitis mice model. We found that DSS-treated mice developed severe inflammation of colon, but became much healthy when intraperitoneal injection with iron. Iron supplementation alleviated colonic and systemic inflammation by lower histological scores, restorative morphology of colonic villi, and reduced expression of pro-inflammatory cytokines. Moreover, intraperitoneal supplementation of iron enhanced intestinal barrier function by upregulating the colonic expressions of tight junction proteins, restoring intestinal immune homeostasis by regulating immune cell infiltration and T lymphocyte subsets, and increasing mucous secretion of goblet cells in the colon. High-throughput sequencing of fecal 16 S rRNA showed that iron injection significantly increased the relative abundance of Bacteroidetes, which was suppressed in the gut microbiota of DSS-induced colitis mice. These results provided evidences supporting the protective effects of systemic iron repletion by intraperitoneal injection of iron on intestinal barrier functions. The finding highlights a novel approach for the treatment of IBD with iron injection therapy.
format	article
author	Li Liang Qingqing Xiong Jingxia Kong Chenying Tian Linfeng Miao Xiaofeng Zhang Huahua Du
author_facet	Li Liang Qingqing Xiong Jingxia Kong Chenying Tian Linfeng Miao Xiaofeng Zhang Huahua Du
author_sort	Li Liang
title	Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
title_short	Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
title_full	Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
title_fullStr	Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
title_full_unstemmed	Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
title_sort	intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/93e3fb2e471a41af9d7fad5eb33a0332
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Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function

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