Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury

Context: Sulphoraphane (SFN) is an isothiocyanate, having antioxidant activity, antitumor, and therapeutic effects on cardiovascular disease. Objective: This study explores the mechanisms of SFN preconditioning on ischaemia/reperfusion injury (IRI). Materials and methods: Cardiomyocytes were divided...

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Autores principales:	Na Peng, Luping Jin, Aizhen He, Changjin Deng, Xiaoqin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	nrf2/ho-1 oxidative stress apoptosis reactive oxygen species mitochondrial membrane potential Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/93e618c0660744bd83ff35f0d950689d
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spelling	oai:doaj.org-article:93e618c0660744bd83ff35f0d950689d2021-11-17T14:21:56ZEffect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury1388-02091744-511610.1080/13880209.2019.1680705https://doaj.org/article/93e618c0660744bd83ff35f0d950689d2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1680705https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Sulphoraphane (SFN) is an isothiocyanate, having antioxidant activity, antitumor, and therapeutic effects on cardiovascular disease. Objective: This study explores the mechanisms of SFN preconditioning on ischaemia/reperfusion injury (IRI). Materials and methods: Cardiomyocytes were divided into four groups as follows: control group (normoxic condition), SFN group (5 μmol/L), hypoxia/reoxygenation (H/R) group (1 h, 3 h) and SFN + H/R group. Cell viability was determined by MTT method. Levels of creatine kinase (CK), nitric oxide (NO), superoxide dismutase (SOD) and maleic dialdehyde (MDA) were determined by colorimetric method. Cell apoptosis, levels of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were determined by flow cytometry. Levels of Bax, Bcl-2, C caspase-3, NF-E2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1) were detected by Western blot. Results: H/R model inhibited cell viability, increased the levels of LDH, CK, Bax and C caspase-3, and decreased the levels of NO, Bcl-2, while the effect of H/R was partially reversed by SFN. SFN treatment reduced ROS, MDA (from 4.9 nM to 2.8 nM) production, elevated SOD level (from 39.5 U/mL to 61.7 U/mL) and improved MMP damage. Under the effect of SFN, up-regulation of nuclear Nrf2 expression and down-regulation of cytosolic Nrf2 expression were observed, which led to Nrf2 nuclear translocation and enhanced the expression of HO-1. Conclusion: These results suggested that SFN had a protective effect on cardiomyocytes undergoing IRI, and its mechanism may be realized via activating the Nrf2/HO-1 pathway, thereby inhibiting apoptosis. This might provide a new approach for the treatment of ischaemic heart disease.Na PengLuping JinAizhen HeChangjin DengXiaoqin WangTaylor & Francis Grouparticlenrf2/ho-1oxidative stressapoptosisreactive oxygen speciesmitochondrial membrane potentialTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 753-759 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	nrf2/ho-1 oxidative stress apoptosis reactive oxygen species mitochondrial membrane potential Therapeutics. Pharmacology RM1-950
spellingShingle	nrf2/ho-1 oxidative stress apoptosis reactive oxygen species mitochondrial membrane potential Therapeutics. Pharmacology RM1-950 Na Peng Luping Jin Aizhen He Changjin Deng Xiaoqin Wang Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
description	Context: Sulphoraphane (SFN) is an isothiocyanate, having antioxidant activity, antitumor, and therapeutic effects on cardiovascular disease. Objective: This study explores the mechanisms of SFN preconditioning on ischaemia/reperfusion injury (IRI). Materials and methods: Cardiomyocytes were divided into four groups as follows: control group (normoxic condition), SFN group (5 μmol/L), hypoxia/reoxygenation (H/R) group (1 h, 3 h) and SFN + H/R group. Cell viability was determined by MTT method. Levels of creatine kinase (CK), nitric oxide (NO), superoxide dismutase (SOD) and maleic dialdehyde (MDA) were determined by colorimetric method. Cell apoptosis, levels of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were determined by flow cytometry. Levels of Bax, Bcl-2, C caspase-3, NF-E2-related factor 2 (Nrf2) and haem oxygenase-1 (HO-1) were detected by Western blot. Results: H/R model inhibited cell viability, increased the levels of LDH, CK, Bax and C caspase-3, and decreased the levels of NO, Bcl-2, while the effect of H/R was partially reversed by SFN. SFN treatment reduced ROS, MDA (from 4.9 nM to 2.8 nM) production, elevated SOD level (from 39.5 U/mL to 61.7 U/mL) and improved MMP damage. Under the effect of SFN, up-regulation of nuclear Nrf2 expression and down-regulation of cytosolic Nrf2 expression were observed, which led to Nrf2 nuclear translocation and enhanced the expression of HO-1. Conclusion: These results suggested that SFN had a protective effect on cardiomyocytes undergoing IRI, and its mechanism may be realized via activating the Nrf2/HO-1 pathway, thereby inhibiting apoptosis. This might provide a new approach for the treatment of ischaemic heart disease.
format	article
author	Na Peng Luping Jin Aizhen He Changjin Deng Xiaoqin Wang
author_facet	Na Peng Luping Jin Aizhen He Changjin Deng Xiaoqin Wang
author_sort	Na Peng
title	Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
title_short	Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
title_full	Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
title_fullStr	Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
title_full_unstemmed	Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
title_sort	effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/93e618c0660744bd83ff35f0d950689d
work_keys_str_mv	AT napeng effectofsulphoraphaneonnewbornmousecardiomyocytesundergoingischaemiareperfusioninjury AT lupingjin effectofsulphoraphaneonnewbornmousecardiomyocytesundergoingischaemiareperfusioninjury AT aizhenhe effectofsulphoraphaneonnewbornmousecardiomyocytesundergoingischaemiareperfusioninjury AT changjindeng effectofsulphoraphaneonnewbornmousecardiomyocytesundergoingischaemiareperfusioninjury AT xiaoqinwang effectofsulphoraphaneonnewbornmousecardiomyocytesundergoingischaemiareperfusioninjury
_version_	1718425496996282368

Effect of sulphoraphane on newborn mouse cardiomyocytes undergoing ischaemia/reperfusion injury

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