Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer

Introduction Ovarian cancer (OC) is one of the most common malignancies and the leading cause of cancer-related death among women. The long non-coding RNA Prostate cancer-associated transcript-1 (PCAT-1) has been reported to play important roles in multiple human cancers. However, the role of PCAT-1...

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Autores principales:	Li-Ping Gu, Shuo Jin, Rong-Chun Xu, Jing Zhang, Ying-Chun Geng, Xing-Yue Shao, Li-Bo Qin
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	ovarian cancer mir-129-5p long non coding rnas pcat-1 long non-coding rna prostate cancer-associated transcript-1 Medicine R
Acceso en línea:	https://doaj.org/article/93e84879755a4c8cb31994c603c07f33
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spelling	oai:doaj.org-article:93e84879755a4c8cb31994c603c07f332021-12-02T18:39:09ZLong non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer1734-19221896-915110.5114/aoms.2018.75534https://doaj.org/article/93e84879755a4c8cb31994c603c07f332019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Long-non-coding-RNA-PCAT-1-promotes-tumor-progression-by-inhibiting-miR-129-5p-in,81368,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Ovarian cancer (OC) is one of the most common malignancies and the leading cause of cancer-related death among women. The long non-coding RNA Prostate cancer-associated transcript-1 (PCAT-1) has been reported to play important roles in multiple human cancers. However, the role of PCAT-1 in OC has never been investigated. The purpose of this study was to investigate the expression and roles of PCAT-1 in OC. Material and methods Expression of PCAT-1 and miR-129-5p in OC tissues and cell lines was determined by qRT-PCR. Cell proliferation and apoptosis were analyzed by MTT assay and flow cytometry, respectively. The interaction between PCAT-1 and miR-129-5p was demonstrated by luciferase reporter assay. Results PCAT-1 is significantly upregulated in OC tissues and cell lines (p < 0.05). Overexpression of PCAT-1 promotes proliferation of OC cells and inhibits their apoptosis (p < 0.05). In addition, miR-129-5p is markedly downregulated in OC and its level is inversely correlated with PCAT-1 expression in OC tumor tissues (p < 0.05). miR-129-5p inhibits proliferation and induces apoptosis in OC cell lines (p < 0.05). Furthermore, it has been demonstrated that miR-129-5p is directly targeted by PCAT-1 and miR-129-5p overexpression can effectively attenuate the effects of PCAT-1 on the proliferation and apoptosis of OC cells. Conclusions Our results suggest that PCAT-1 functions as an oncogene by inhibiting miR-129-5p in OC and silencing PCAT-1 may be a novel therapeutic strategy in the treatment of OC.Li-Ping GuShuo JinRong-Chun XuJing ZhangYing-Chun GengXing-Yue ShaoLi-Bo QinTermedia Publishing Housearticleovarian cancermir-129-5plong non coding rnaspcat-1long non-coding rnaprostate cancer-associated transcript-1MedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 513-521 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovarian cancer mir-129-5p long non coding rnas pcat-1 long non-coding rna prostate cancer-associated transcript-1 Medicine R
spellingShingle	ovarian cancer mir-129-5p long non coding rnas pcat-1 long non-coding rna prostate cancer-associated transcript-1 Medicine R Li-Ping Gu Shuo Jin Rong-Chun Xu Jing Zhang Ying-Chun Geng Xing-Yue Shao Li-Bo Qin Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
description	Introduction Ovarian cancer (OC) is one of the most common malignancies and the leading cause of cancer-related death among women. The long non-coding RNA Prostate cancer-associated transcript-1 (PCAT-1) has been reported to play important roles in multiple human cancers. However, the role of PCAT-1 in OC has never been investigated. The purpose of this study was to investigate the expression and roles of PCAT-1 in OC. Material and methods Expression of PCAT-1 and miR-129-5p in OC tissues and cell lines was determined by qRT-PCR. Cell proliferation and apoptosis were analyzed by MTT assay and flow cytometry, respectively. The interaction between PCAT-1 and miR-129-5p was demonstrated by luciferase reporter assay. Results PCAT-1 is significantly upregulated in OC tissues and cell lines (p < 0.05). Overexpression of PCAT-1 promotes proliferation of OC cells and inhibits their apoptosis (p < 0.05). In addition, miR-129-5p is markedly downregulated in OC and its level is inversely correlated with PCAT-1 expression in OC tumor tissues (p < 0.05). miR-129-5p inhibits proliferation and induces apoptosis in OC cell lines (p < 0.05). Furthermore, it has been demonstrated that miR-129-5p is directly targeted by PCAT-1 and miR-129-5p overexpression can effectively attenuate the effects of PCAT-1 on the proliferation and apoptosis of OC cells. Conclusions Our results suggest that PCAT-1 functions as an oncogene by inhibiting miR-129-5p in OC and silencing PCAT-1 may be a novel therapeutic strategy in the treatment of OC.
format	article
author	Li-Ping Gu Shuo Jin Rong-Chun Xu Jing Zhang Ying-Chun Geng Xing-Yue Shao Li-Bo Qin
author_facet	Li-Ping Gu Shuo Jin Rong-Chun Xu Jing Zhang Ying-Chun Geng Xing-Yue Shao Li-Bo Qin
author_sort	Li-Ping Gu
title	Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
title_short	Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
title_full	Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
title_fullStr	Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
title_full_unstemmed	Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer
title_sort	long non-coding rna pcat-1 promotes tumor progression by inhibiting mir-129-5p in human ovarian cancer
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/93e84879755a4c8cb31994c603c07f33
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Long non-coding RNA PCAT-1 promotes tumor progression by inhibiting miR-129-5p in human ovarian cancer

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