Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep

Abstract. Haren HIH, Purwantini D, Sumaryadi MY, Prayitno. 2020. Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep. Biodiversitas 21: 2074-2078. The present study aimed to investigate the association between myosta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hassan Ishag Hassan Haren, Dattadewi Purwantini, Mas Yedi Sumaryadi, Prayitno Prayitno
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	batur sheep, carcass traits, growth traits, mstn gene, third exon Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/93f6e7362e78491297cf36bcab153e2a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:93f6e7362e78491297cf36bcab153e2a
record_format	dspace
spelling	oai:doaj.org-article:93f6e7362e78491297cf36bcab153e2a2021-11-22T00:35:33ZShort Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep1412-033X2085-472210.13057/biodiv/d210534https://doaj.org/article/93f6e7362e78491297cf36bcab153e2a2020-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5438https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Haren HIH, Purwantini D, Sumaryadi MY, Prayitno. 2020. Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep. Biodiversitas 21: 2074-2078. The present study aimed to investigate the association between myostatin (MSTN) genotype and two traits, growth and carcass, in Batur sheep. Blood samples representing thirty head were collected and genomic DNA was extracted. A specific primer designed to amplify the MSTN gene, samples sequenced then, used the BioEdit program to identify any mutation. Calculation of genotypes, gene and allele frequencies, heterozygosities, and Chi-square test was performed. Only two alleles observed (G and C) resulted in three genotypes. 11 polymorphic sites were observed, transversion at c.121G>C, and one individual G>A which disrupted reading frame of whole MSTN sequenced, genotypic and allelic frequencies were 0.552 GG, 0.379 GC, and 0.069 CC, where the allele frequency was 0.741 G and 0.259 C. Lambs that carrying genotype GC had slightly more pre-slaughter weight, hot carcass weight, cold carcass weight, carcass length, and total yield (lion, shoulder, leg yield, and thigh) compared to those carrying genotype GG. There was no significant effect of the MSTN genotype on carcass traits (p=0.05). Polymorphic site c.121 G>C is present in Batur sheep for the first time about the association with the MSTN gene however, it has not effects weaning weight, 6-month weight, and studied carcass traits.Hassan Ishag Hassan HarenDattadewi PurwantiniMas Yedi SumaryadiPrayitno PrayitnoMBI & UNS Soloarticlebatur sheep, carcass traits, growth traits, mstn gene, third exonBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	batur sheep, carcass traits, growth traits, mstn gene, third exon Biology (General) QH301-705.5
spellingShingle	batur sheep, carcass traits, growth traits, mstn gene, third exon Biology (General) QH301-705.5 Hassan Ishag Hassan Haren Dattadewi Purwantini Mas Yedi Sumaryadi Prayitno Prayitno Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
description	Abstract. Haren HIH, Purwantini D, Sumaryadi MY, Prayitno. 2020. Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep. Biodiversitas 21: 2074-2078. The present study aimed to investigate the association between myostatin (MSTN) genotype and two traits, growth and carcass, in Batur sheep. Blood samples representing thirty head were collected and genomic DNA was extracted. A specific primer designed to amplify the MSTN gene, samples sequenced then, used the BioEdit program to identify any mutation. Calculation of genotypes, gene and allele frequencies, heterozygosities, and Chi-square test was performed. Only two alleles observed (G and C) resulted in three genotypes. 11 polymorphic sites were observed, transversion at c.121G>C, and one individual G>A which disrupted reading frame of whole MSTN sequenced, genotypic and allelic frequencies were 0.552 GG, 0.379 GC, and 0.069 CC, where the allele frequency was 0.741 G and 0.259 C. Lambs that carrying genotype GC had slightly more pre-slaughter weight, hot carcass weight, cold carcass weight, carcass length, and total yield (lion, shoulder, leg yield, and thigh) compared to those carrying genotype GG. There was no significant effect of the MSTN genotype on carcass traits (p=0.05). Polymorphic site c.121 G>C is present in Batur sheep for the first time about the association with the MSTN gene however, it has not effects weaning weight, 6-month weight, and studied carcass traits.
format	article
author	Hassan Ishag Hassan Haren Dattadewi Purwantini Mas Yedi Sumaryadi Prayitno Prayitno
author_facet	Hassan Ishag Hassan Haren Dattadewi Purwantini Mas Yedi Sumaryadi Prayitno Prayitno
author_sort	Hassan Ishag Hassan Haren
title	Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
title_short	Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
title_full	Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
title_fullStr	Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
title_full_unstemmed	Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep
title_sort	short communication: polymorphism at third exon of the myostatin gene and its association with growth and carcass traits in batur sheep
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/93f6e7362e78491297cf36bcab153e2a
work_keys_str_mv	AT hassanishaghassanharen shortcommunicationpolymorphismatthirdexonofthemyostatingeneanditsassociationwithgrowthandcarcasstraitsinbatursheep AT dattadewipurwantini shortcommunicationpolymorphismatthirdexonofthemyostatingeneanditsassociationwithgrowthandcarcasstraitsinbatursheep AT masyedisumaryadi shortcommunicationpolymorphismatthirdexonofthemyostatingeneanditsassociationwithgrowthandcarcasstraitsinbatursheep AT prayitnoprayitno shortcommunicationpolymorphismatthirdexonofthemyostatingeneanditsassociationwithgrowthandcarcasstraitsinbatursheep
_version_	1718418538098589696

Short Communication: Polymorphism at third exon of the Myostatin gene and its association with growth and carcass traits in Batur sheep

Ejemplares similares