Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model

Abstract Pluripotent stem cell-derived cardiomyocytes show great promise in regenerating the heart after myocardial infarction; however, several uncertainties exist that must be addressed before clinical trials. One practical issue is graft survival following transplantation. Although a pro-survival...

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Autores principales:	Tatsuki Ogasawara, Satomi Okano, Hajime Ichimura, Shin Kadota, Yuki Tanaka, Itsunari Minami, Motonari Uesugi, Yuko Wada, Naoto Saito, Kenji Okada, Koichiro Kuwahara, Yuji Shiba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93fb020c748746f585e41a522d5a3a4e
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spelling	oai:doaj.org-article:93fb020c748746f585e41a522d5a3a4e2021-12-02T16:06:32ZImpact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model10.1038/s41598-017-09217-x2045-2322https://doaj.org/article/93fb020c748746f585e41a522d5a3a4e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09217-xhttps://doaj.org/toc/2045-2322Abstract Pluripotent stem cell-derived cardiomyocytes show great promise in regenerating the heart after myocardial infarction; however, several uncertainties exist that must be addressed before clinical trials. One practical issue is graft survival following transplantation. Although a pro-survival cocktail with Matrigel has been shown to enhance graft survival, the use of Matrigel may not be clinically feasible. The purpose of this study was to test whether a hyaluronan-based hydrogel, HyStem, could be a substitute for Matrigel. Human induced pluripotent stem cell-derived cardiomyocytes diluted with HyStem alone, HyStem plus pro-survival factors, or a pro-survival cocktail with Matrigel (PSC/MG), were transplanted into a rat model of acute myocardial infarction. Histological analysis at 4 weeks post transplantation revealed that, among the three groups, recipients of PSC/MG showed the largest graft size. Additionally, the grafted cardiomyocytes in the recipients of PSC/MG had a more matured phenotype compared to those in the other two groups. These findings suggest that further studies will be required to enhance not only graft size, but also the maturation of grafted cardiomyocytes.Tatsuki OgasawaraSatomi OkanoHajime IchimuraShin KadotaYuki TanakaItsunari MinamiMotonari UesugiYuko WadaNaoto SaitoKenji OkadaKoichiro KuwaharaYuji ShibaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tatsuki Ogasawara Satomi Okano Hajime Ichimura Shin Kadota Yuki Tanaka Itsunari Minami Motonari Uesugi Yuko Wada Naoto Saito Kenji Okada Koichiro Kuwahara Yuji Shiba Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
description	Abstract Pluripotent stem cell-derived cardiomyocytes show great promise in regenerating the heart after myocardial infarction; however, several uncertainties exist that must be addressed before clinical trials. One practical issue is graft survival following transplantation. Although a pro-survival cocktail with Matrigel has been shown to enhance graft survival, the use of Matrigel may not be clinically feasible. The purpose of this study was to test whether a hyaluronan-based hydrogel, HyStem, could be a substitute for Matrigel. Human induced pluripotent stem cell-derived cardiomyocytes diluted with HyStem alone, HyStem plus pro-survival factors, or a pro-survival cocktail with Matrigel (PSC/MG), were transplanted into a rat model of acute myocardial infarction. Histological analysis at 4 weeks post transplantation revealed that, among the three groups, recipients of PSC/MG showed the largest graft size. Additionally, the grafted cardiomyocytes in the recipients of PSC/MG had a more matured phenotype compared to those in the other two groups. These findings suggest that further studies will be required to enhance not only graft size, but also the maturation of grafted cardiomyocytes.
format	article
author	Tatsuki Ogasawara Satomi Okano Hajime Ichimura Shin Kadota Yuki Tanaka Itsunari Minami Motonari Uesugi Yuko Wada Naoto Saito Kenji Okada Koichiro Kuwahara Yuji Shiba
author_facet	Tatsuki Ogasawara Satomi Okano Hajime Ichimura Shin Kadota Yuki Tanaka Itsunari Minami Motonari Uesugi Yuko Wada Naoto Saito Kenji Okada Koichiro Kuwahara Yuji Shiba
author_sort	Tatsuki Ogasawara
title	Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
title_short	Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
title_full	Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
title_fullStr	Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
title_full_unstemmed	Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
title_sort	impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/93fb020c748746f585e41a522d5a3a4e
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Impact of extracellular matrix on engraftment and maturation of pluripotent stem cell-derived cardiomyocytes in a rat myocardial infarct model

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