Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses

Abstract G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter. Folding of HIV-1 LTR G4s inhibits viral transcription; stabilization...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rosalba Perrone, Enrico Lavezzo, Giorgio Palù, Sara N. Richter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/93fd701a8a2743ff8827e3e7d8a9ac41
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:93fd701a8a2743ff8827e3e7d8a9ac41
record_format	dspace
spelling	oai:doaj.org-article:93fd701a8a2743ff8827e3e7d8a9ac412021-12-02T16:06:09ZConserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses10.1038/s41598-017-02291-12045-2322https://doaj.org/article/93fd701a8a2743ff8827e3e7d8a9ac412017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02291-1https://doaj.org/toc/2045-2322Abstract G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter. Folding of HIV-1 LTR G4s inhibits viral transcription; stabilization by G4 ligands intensifies this effect. Cellular proteins modulate viral transcription by inducing/unfolding LTR G4s. We here expanded our investigation on the presence of LTR G4s to all lentiviruses. G4s in the 5′-LTR U3 region were completely conserved in primate lentiviruses. A G4 was also present in a cattle-infecting lentivirus. All other non-primate lentiviruses displayed hints of less stable G4s. In primate lentiviruses, the possibility to fold into G4s was highly conserved among strains. LTR G4 sequences were very similar among phylogenetically related primate viruses, while they increasingly differed in viruses that diverged early from a common ancestor. A strong correlation between primate lentivirus LTR G4s and Sp1/NFκB binding sites was found. All LTR G4s folded: their complexity was assessed by polymerase stop assay. Our data support a role of the lentiviruses 5′-LTR G4 region as control centre of viral transcription, where folding/unfolding of G4s and multiple recruitment of factors based on both sequence and structure may take place.Rosalba PerroneEnrico LavezzoGiorgio PalùSara N. RichterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rosalba Perrone Enrico Lavezzo Giorgio Palù Sara N. Richter Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
description	Abstract G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter. Folding of HIV-1 LTR G4s inhibits viral transcription; stabilization by G4 ligands intensifies this effect. Cellular proteins modulate viral transcription by inducing/unfolding LTR G4s. We here expanded our investigation on the presence of LTR G4s to all lentiviruses. G4s in the 5′-LTR U3 region were completely conserved in primate lentiviruses. A G4 was also present in a cattle-infecting lentivirus. All other non-primate lentiviruses displayed hints of less stable G4s. In primate lentiviruses, the possibility to fold into G4s was highly conserved among strains. LTR G4 sequences were very similar among phylogenetically related primate viruses, while they increasingly differed in viruses that diverged early from a common ancestor. A strong correlation between primate lentivirus LTR G4s and Sp1/NFκB binding sites was found. All LTR G4s folded: their complexity was assessed by polymerase stop assay. Our data support a role of the lentiviruses 5′-LTR G4 region as control centre of viral transcription, where folding/unfolding of G4s and multiple recruitment of factors based on both sequence and structure may take place.
format	article
author	Rosalba Perrone Enrico Lavezzo Giorgio Palù Sara N. Richter
author_facet	Rosalba Perrone Enrico Lavezzo Giorgio Palù Sara N. Richter
author_sort	Rosalba Perrone
title	Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
title_short	Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
title_full	Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
title_fullStr	Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
title_full_unstemmed	Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses
title_sort	conserved presence of g-quadruplex forming sequences in the long terminal repeat promoter of lentiviruses
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/93fd701a8a2743ff8827e3e7d8a9ac41
work_keys_str_mv	AT rosalbaperrone conservedpresenceofgquadruplexformingsequencesinthelongterminalrepeatpromoteroflentiviruses AT enricolavezzo conservedpresenceofgquadruplexformingsequencesinthelongterminalrepeatpromoteroflentiviruses AT giorgiopalu conservedpresenceofgquadruplexformingsequencesinthelongterminalrepeatpromoteroflentiviruses AT saranrichter conservedpresenceofgquadruplexformingsequencesinthelongterminalrepeatpromoteroflentiviruses
_version_	1718385102440890368

Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses

Ejemplares similares