Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

Abstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitr...

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Autores principales:	Zi Ye, Tongrui Sun, Huoye Hao, Yanling He, Xueyan Liu, Minrui Guo, Guogang Chen
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Carbon sources C/N ratio Lipid production Nitrogen sources Rhodosporidium toruloides Biotechnology TP248.13-248.65 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9403f59437d44bca847a3b0859f6079e
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spelling	oai:doaj.org-article:9403f59437d44bca847a3b0859f6079e2021-11-21T12:09:02ZOptimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production10.1186/s13568-021-01313-62191-0855https://doaj.org/article/9403f59437d44bca847a3b0859f6079e2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13568-021-01313-6https://doaj.org/toc/2191-0855Abstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.Zi YeTongrui SunHuoye HaoYanling HeXueyan LiuMinrui GuoGuogang ChenSpringerOpenarticleCarbon sourcesC/N ratioLipid productionNitrogen sourcesRhodosporidium toruloidesBiotechnologyTP248.13-248.65MicrobiologyQR1-502ENAMB Express, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon sources C/N ratio Lipid production Nitrogen sources Rhodosporidium toruloides Biotechnology TP248.13-248.65 Microbiology QR1-502
spellingShingle	Carbon sources C/N ratio Lipid production Nitrogen sources Rhodosporidium toruloides Biotechnology TP248.13-248.65 Microbiology QR1-502 Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
description	Abstract Rhodosporidium toruloides is a useful oleaginous yeast, but lipids production is affected by various factors including nutrients in the culture medium. Herein, the R-ZL2 high-yield mutant strain was used to investigate the effects of different carbon sources (sucrose, glucose, xylose), nitrogen sources (ammonium sulphate, ammonium nitrate), and C/N ratio on lipids production capacity, get the following conclusion (1) Compared with glucose and xylose, sucrose was a superior carbon source for lipids production; (2) When using ammonium sulphate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (10.7 g/L, 6.32 g/L and 59%, respectively), and lipids produced under different C/N conditions have potential for biodiesel production (except for C/N = 40 and C/N = 80); (3) When using ammonium nitrate as the nitrogen source, a C/N ratio of 200:1 achieved the highest biomass, lipids production and lipids content (12.1 g/L, 8.25 g/L and 65%, respectively), and lipids produced under different C/N ratio conditions have potential for biodiesel production. Thus, a combination of sucrose and ammonium nitrate was optimal for the lipid accumulation in R-ZL2. The findings will lay a foundation for further improving lipids yields.
format	article
author	Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen
author_facet	Zi Ye Tongrui Sun Huoye Hao Yanling He Xueyan Liu Minrui Guo Guogang Chen
author_sort	Zi Ye
title	Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
title_short	Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
title_full	Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
title_fullStr	Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
title_full_unstemmed	Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production
title_sort	optimising nutrients in the culture medium of rhodosporidium toruloides enhances lipids production
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/9403f59437d44bca847a3b0859f6079e
work_keys_str_mv	AT ziye optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT tongruisun optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT huoyehao optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT yanlinghe optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT xueyanliu optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT minruiguo optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction AT guogangchen optimisingnutrientsintheculturemediumofrhodosporidiumtoruloidesenhanceslipidsproduction
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Optimising nutrients in the culture medium of Rhodosporidium toruloides enhances lipids production

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