Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece

Abstract Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously...

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Autores principales:	Panos Bravakos, Manolis Mandalakis, Paraskevi Nomikou, Thekla I. Anastasiou, Jon Bent Kristoffersen, Melanthia Stavroulaki, Stephanos Kilias, Georgios Kotoulas, Antonios Magoulas, Paraskevi N. Polymenakou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94054e49d0984368a154f91b0490ce37
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spelling	oai:doaj.org-article:94054e49d0984368a154f91b0490ce372021-12-02T14:12:07ZGenomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece10.1038/s41598-020-79359-y2045-2322https://doaj.org/article/94054e49d0984368a154f91b0490ce372021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79359-yhttps://doaj.org/toc/2045-2322Abstract Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously isolated from an active submarine volcano of Greece, the Kolumbo volcano. Our goal was to identify the genetic basis of the enhanced co-tolerance to antibiotics and acidity of these Pseudomonas strains. Pangenome analysis identified 10,908 Gene Clusters (GCs). It revealed that the numbers of phage-related GCs and sigma factors, which both provide the mechanisms of adaptation to environmental stressors, were much higher in the high tolerant Pseudomonas strains compared to the rest ones. All identified GCs of these strains were associated with antimicrobial and multidrug resistance. The present study provides strong evidence that the CO2-rich seawater of the volcano associated with low pH might be a reservoir of microorganisms carrying multidrug efflux-mediated systems and pumps. We, therefore, suggest further studies of other extreme environments (or ecosystems) and their associated physicochemical parameters (or factors) in the rise of antibiotic resistance.Panos BravakosManolis MandalakisParaskevi NomikouThekla I. AnastasiouJon Bent KristoffersenMelanthia StavroulakiStephanos KiliasGeorgios KotoulasAntonios MagoulasParaskevi N. PolymenakouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Panos Bravakos Manolis Mandalakis Paraskevi Nomikou Thekla I. Anastasiou Jon Bent Kristoffersen Melanthia Stavroulaki Stephanos Kilias Georgios Kotoulas Antonios Magoulas Paraskevi N. Polymenakou Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
description	Abstract Although the rise of antibiotic and multidrug resistant bacteria is one of the biggest current threats to human health, our understanding of the mechanisms involved in antibiotic resistance selection remains scarce. We performed whole genome sequencing of 21 Pseudomonas strains, previously isolated from an active submarine volcano of Greece, the Kolumbo volcano. Our goal was to identify the genetic basis of the enhanced co-tolerance to antibiotics and acidity of these Pseudomonas strains. Pangenome analysis identified 10,908 Gene Clusters (GCs). It revealed that the numbers of phage-related GCs and sigma factors, which both provide the mechanisms of adaptation to environmental stressors, were much higher in the high tolerant Pseudomonas strains compared to the rest ones. All identified GCs of these strains were associated with antimicrobial and multidrug resistance. The present study provides strong evidence that the CO2-rich seawater of the volcano associated with low pH might be a reservoir of microorganisms carrying multidrug efflux-mediated systems and pumps. We, therefore, suggest further studies of other extreme environments (or ecosystems) and their associated physicochemical parameters (or factors) in the rise of antibiotic resistance.
format	article
author	Panos Bravakos Manolis Mandalakis Paraskevi Nomikou Thekla I. Anastasiou Jon Bent Kristoffersen Melanthia Stavroulaki Stephanos Kilias Georgios Kotoulas Antonios Magoulas Paraskevi N. Polymenakou
author_facet	Panos Bravakos Manolis Mandalakis Paraskevi Nomikou Thekla I. Anastasiou Jon Bent Kristoffersen Melanthia Stavroulaki Stephanos Kilias Georgios Kotoulas Antonios Magoulas Paraskevi N. Polymenakou
author_sort	Panos Bravakos
title	Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
title_short	Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
title_full	Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
title_fullStr	Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
title_full_unstemmed	Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece
title_sort	genomic adaptation of pseudomonas strains to acidity and antibiotics in hydrothermal vents at kolumbo submarine volcano, greece
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94054e49d0984368a154f91b0490ce37
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Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece

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