Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.

Genetic recombination contributes to the diversity of human immunodeficiency virus (HIV-1). Productive HIV-1 recombination is, however, dependent on both the number of HIV-1 genomes per infected cell and the genetic relationship between these viral genomes. A detailed analysis of the number of provi...

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Autores principales:	Lina Josefsson, Sarah Palmer, Nuno R Faria, Philippe Lemey, Joseph Casazza, David Ambrozak, Mary Kearney, Wei Shao, Shyamasundaran Kottilil, Michael Sneller, John Mellors, John M Coffin, Frank Maldarelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/940b3bbf102643bc9774ff04f63b1984
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spelling	oai:doaj.org-article:940b3bbf102643bc9774ff04f63b19842021-11-18T06:05:30ZSingle cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.1553-73661553-737410.1371/journal.ppat.1003432https://doaj.org/article/940b3bbf102643bc9774ff04f63b19842013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23818847/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Genetic recombination contributes to the diversity of human immunodeficiency virus (HIV-1). Productive HIV-1 recombination is, however, dependent on both the number of HIV-1 genomes per infected cell and the genetic relationship between these viral genomes. A detailed analysis of the number of proviruses and their genetic relationship in infected cells isolated from peripheral blood and tissue compartments is therefore important for understanding HIV-1 recombination, genetic diversity and the dynamics of HIV-1 infection. To address these issues, we used a previously developed single-cell sequencing technique to quantify and genetically characterize individual HIV-1 DNA molecules from single cells in lymph node tissue and peripheral blood. Analysis of memory and naïve CD4(+) T cells from paired lymph node and peripheral blood samples from five untreated chronically infected patients revealed that the majority of these HIV-1-infected cells (>90%) contain only one copy of HIV-1 DNA, implying a limited potential for productive recombination in virus produced by these cells in these two compartments. Phylogenetic analysis revealed genetic similarity of HIV-1 DNA in memory and naïve CD4(+) T-cells from lymph node, peripheral blood and HIV-1 RNA from plasma, implying exchange of virus and/or infected cells between these compartments in untreated chronic infection.Lina JosefssonSarah PalmerNuno R FariaPhilippe LemeyJoseph CasazzaDavid AmbrozakMary KearneyWei ShaoShyamasundaran KottililMichael SnellerJohn MellorsJohn M CoffinFrank MaldarelliPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 6, p e1003432 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Lina Josefsson Sarah Palmer Nuno R Faria Philippe Lemey Joseph Casazza David Ambrozak Mary Kearney Wei Shao Shyamasundaran Kottilil Michael Sneller John Mellors John M Coffin Frank Maldarelli Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
description	Genetic recombination contributes to the diversity of human immunodeficiency virus (HIV-1). Productive HIV-1 recombination is, however, dependent on both the number of HIV-1 genomes per infected cell and the genetic relationship between these viral genomes. A detailed analysis of the number of proviruses and their genetic relationship in infected cells isolated from peripheral blood and tissue compartments is therefore important for understanding HIV-1 recombination, genetic diversity and the dynamics of HIV-1 infection. To address these issues, we used a previously developed single-cell sequencing technique to quantify and genetically characterize individual HIV-1 DNA molecules from single cells in lymph node tissue and peripheral blood. Analysis of memory and naïve CD4(+) T cells from paired lymph node and peripheral blood samples from five untreated chronically infected patients revealed that the majority of these HIV-1-infected cells (>90%) contain only one copy of HIV-1 DNA, implying a limited potential for productive recombination in virus produced by these cells in these two compartments. Phylogenetic analysis revealed genetic similarity of HIV-1 DNA in memory and naïve CD4(+) T-cells from lymph node, peripheral blood and HIV-1 RNA from plasma, implying exchange of virus and/or infected cells between these compartments in untreated chronic infection.
format	article
author	Lina Josefsson Sarah Palmer Nuno R Faria Philippe Lemey Joseph Casazza David Ambrozak Mary Kearney Wei Shao Shyamasundaran Kottilil Michael Sneller John Mellors John M Coffin Frank Maldarelli
author_facet	Lina Josefsson Sarah Palmer Nuno R Faria Philippe Lemey Joseph Casazza David Ambrozak Mary Kearney Wei Shao Shyamasundaran Kottilil Michael Sneller John Mellors John M Coffin Frank Maldarelli
author_sort	Lina Josefsson
title	Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
title_short	Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
title_full	Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
title_fullStr	Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
title_full_unstemmed	Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.
title_sort	single cell analysis of lymph node tissue from hiv-1 infected patients reveals that the majority of cd4+ t-cells contain one hiv-1 dna molecule.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/940b3bbf102643bc9774ff04f63b1984
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Single cell analysis of lymph node tissue from HIV-1 infected patients reveals that the majority of CD4+ T-cells contain one HIV-1 DNA molecule.

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