Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans

Abstract To assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibi...

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Autores principales:	Tomomi Komura, Mikihiro Yamanaka, Kohji Nishimura, Keita Hara, Yoshikazu Nishikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/940c30c7908e44b69bc4ef3ed9ecd63a
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spelling	oai:doaj.org-article:940c30c7908e44b69bc4ef3ed9ecd63a2021-12-02T17:52:21ZAutofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans10.1038/s41514-021-00061-y2056-3973https://doaj.org/article/940c30c7908e44b69bc4ef3ed9ecd63a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41514-021-00061-yhttps://doaj.org/toc/2056-3973Abstract To assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibited longer life expectancy. When proteins extracted from worms were incubated with sugars, the fluorescence intensity and the concentration of advanced glycation end products (AGEs) increased over time. Ribose enhanced these changes not only in vitro but also in vivo. The glycation blocker rifampicin suppressed this rise in fluorescence. High-resolution mass spectrometry revealed that vitellogenins accumulated in old worms, and glycated vitellogenins emitted six-fold higher fluorescence than naive vitellogenins. The increase in fluorescence with ageing originates from glycated substances, and therefore could serve as a useful noninvasive biomarker of AGEs. C. elegans can serve as a new model to look for anti-AGE factors and to study the relationship between AGEs and senescence.Tomomi KomuraMikihiro YamanakaKohji NishimuraKeita HaraYoshikazu NishikawaNature PortfolioarticleGeriatricsRC952-954.6ENnpj Aging and Mechanisms of Disease, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Geriatrics RC952-954.6
spellingShingle	Geriatrics RC952-954.6 Tomomi Komura Mikihiro Yamanaka Kohji Nishimura Keita Hara Yoshikazu Nishikawa Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
description	Abstract To assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibited longer life expectancy. When proteins extracted from worms were incubated with sugars, the fluorescence intensity and the concentration of advanced glycation end products (AGEs) increased over time. Ribose enhanced these changes not only in vitro but also in vivo. The glycation blocker rifampicin suppressed this rise in fluorescence. High-resolution mass spectrometry revealed that vitellogenins accumulated in old worms, and glycated vitellogenins emitted six-fold higher fluorescence than naive vitellogenins. The increase in fluorescence with ageing originates from glycated substances, and therefore could serve as a useful noninvasive biomarker of AGEs. C. elegans can serve as a new model to look for anti-AGE factors and to study the relationship between AGEs and senescence.
format	article
author	Tomomi Komura Mikihiro Yamanaka Kohji Nishimura Keita Hara Yoshikazu Nishikawa
author_facet	Tomomi Komura Mikihiro Yamanaka Kohji Nishimura Keita Hara Yoshikazu Nishikawa
author_sort	Tomomi Komura
title	Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
title_short	Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
title_full	Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
title_fullStr	Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
title_full_unstemmed	Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans
title_sort	autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in caenorhabditis elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/940c30c7908e44b69bc4ef3ed9ecd63a
work_keys_str_mv	AT tomomikomura autofluorescenceasanoninvasivebiomarkerofsenescenceandadvancedglycationendproductsincaenorhabditiselegans AT mikihiroyamanaka autofluorescenceasanoninvasivebiomarkerofsenescenceandadvancedglycationendproductsincaenorhabditiselegans AT kohjinishimura autofluorescenceasanoninvasivebiomarkerofsenescenceandadvancedglycationendproductsincaenorhabditiselegans AT keitahara autofluorescenceasanoninvasivebiomarkerofsenescenceandadvancedglycationendproductsincaenorhabditiselegans AT yoshikazunishikawa autofluorescenceasanoninvasivebiomarkerofsenescenceandadvancedglycationendproductsincaenorhabditiselegans
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Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans

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