Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows

Abstract Climate change affects the duration and intensity of heat waves during summer months and jeopardizes animal health and welfare. High ambient temperatures cause heat stress in dairy cows resulting in a reduction of milk yield, feed intake, and alterations in gut barrier function. The objecti...

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Autores principales:	Franziska Koch, Dirk Albrecht, Solvig Görs, Björn Kuhla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/941b80743b2f40e6b12ffbd9b84ba42e
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spelling	oai:doaj.org-article:941b80743b2f40e6b12ffbd9b84ba42e2021-12-02T17:41:04ZJejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows10.1038/s41598-021-92053-x2045-2322https://doaj.org/article/941b80743b2f40e6b12ffbd9b84ba42e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92053-xhttps://doaj.org/toc/2045-2322Abstract Climate change affects the duration and intensity of heat waves during summer months and jeopardizes animal health and welfare. High ambient temperatures cause heat stress in dairy cows resulting in a reduction of milk yield, feed intake, and alterations in gut barrier function. The objectives of this study were to investigate the mucosal amino acid, glucose and lactate metabolism, as well as the proteomic response of the small intestine in heat stressed (HS) Holstein dairy cows. Cows of the HS group (n = 5) were exposed for 4 days to 28 °C (THI = 76) in a climate chamber. Percentage decrease in daily ad libitum intake of HS cows was calculated to provide isocaloric energy intake to pair-fed control cows kept at 15 °C (THI = 60) for 4 days. The metabolite, mRNA and proteomic analyses revealed that HS induced incorrect protein folding, cellular destabilization, increased proteolytic degradation and protein kinase inhibitor activity, reduced glycolysis, and activation of NF-κB signaling, uronate cycling, pentose phosphate pathway, fatty acid and amino acid catabolism, mitochondrial respiration, ATPase activity and the antioxidative defence system. Our results highlight adaptive metabolic and immune mechanisms attempting to maintain the biological function in the small intestine of heat-stressed dairy cows.Franziska KochDirk AlbrechtSolvig GörsBjörn KuhlaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Franziska Koch Dirk Albrecht Solvig Görs Björn Kuhla Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
description	Abstract Climate change affects the duration and intensity of heat waves during summer months and jeopardizes animal health and welfare. High ambient temperatures cause heat stress in dairy cows resulting in a reduction of milk yield, feed intake, and alterations in gut barrier function. The objectives of this study were to investigate the mucosal amino acid, glucose and lactate metabolism, as well as the proteomic response of the small intestine in heat stressed (HS) Holstein dairy cows. Cows of the HS group (n = 5) were exposed for 4 days to 28 °C (THI = 76) in a climate chamber. Percentage decrease in daily ad libitum intake of HS cows was calculated to provide isocaloric energy intake to pair-fed control cows kept at 15 °C (THI = 60) for 4 days. The metabolite, mRNA and proteomic analyses revealed that HS induced incorrect protein folding, cellular destabilization, increased proteolytic degradation and protein kinase inhibitor activity, reduced glycolysis, and activation of NF-κB signaling, uronate cycling, pentose phosphate pathway, fatty acid and amino acid catabolism, mitochondrial respiration, ATPase activity and the antioxidative defence system. Our results highlight adaptive metabolic and immune mechanisms attempting to maintain the biological function in the small intestine of heat-stressed dairy cows.
format	article
author	Franziska Koch Dirk Albrecht Solvig Görs Björn Kuhla
author_facet	Franziska Koch Dirk Albrecht Solvig Görs Björn Kuhla
author_sort	Franziska Koch
title	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
title_short	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
title_full	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
title_fullStr	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
title_full_unstemmed	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
title_sort	jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/941b80743b2f40e6b12ffbd9b84ba42e
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Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows

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