Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium

Abstract The use of microorganisms that allows the recovery of critical high-tech elements such as gallium (Ga) and indium (In) has been considered an excellent eco-strategy. In this perspective, it is relevant to understand the strategies of Ga and In resistant strains to cope with these critical m...

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Autores principales:	Joana B. Caldeira, Paula V. Morais, Rita Branco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/941ca6f4d199473fbd71113cc0c72dbf
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spelling	oai:doaj.org-article:941ca6f4d199473fbd71113cc0c72dbf2021-12-02T12:33:54ZExploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium10.1038/s41598-020-77447-72045-2322https://doaj.org/article/941ca6f4d199473fbd71113cc0c72dbf2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77447-7https://doaj.org/toc/2045-2322Abstract The use of microorganisms that allows the recovery of critical high-tech elements such as gallium (Ga) and indium (In) has been considered an excellent eco-strategy. In this perspective, it is relevant to understand the strategies of Ga and In resistant strains to cope with these critical metals. This study aimed to explore the effect of these metals on two Ga/In resistant strains and to scrutinize the biological processes behind the oxidative stress in response to exposure to these critical metals. Two strains of Serratia fonticola, A3242 and B2A1Ga1, with high resistance to Ga and In, were submitted to metal stress and their protein profiles showed an overexpressed Superoxide Dismutase (SOD) in presence of In. Results of inhibitor-protein native gel incubations identified the overexpressed enzyme as a Fe-SOD. Both strains exhibited a huge increase of oxidative stress when exposed to indium, visible by an extreme high amount of reactive oxygen species (ROS) production. The toxicity induced by indium triggered biological mechanisms of stress control namely, the decrease in reduced glutathione/total glutathione levels and an increase in the SOD activity. The effect of gallium in cells was not so boisterous, visible only by the decrease of reduced glutathione levels. Analysis of the cellular metabolic viability revealed that each strain was affected differently by the critical metals, which could be related to the distinct metal uptakes. Strain A3242 accumulated more Ga and In in comparison to strain B2A1Ga1, and showed lower metabolic activity. Understanding the biological response of the two metal resistant strains of S. fonticola to stress induced by Ga and In will tackle the current gap of information related with bacteria-critical metals interactions.Joana B. CaldeiraPaula V. MoraisRita BrancoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joana B. Caldeira Paula V. Morais Rita Branco Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
description	Abstract The use of microorganisms that allows the recovery of critical high-tech elements such as gallium (Ga) and indium (In) has been considered an excellent eco-strategy. In this perspective, it is relevant to understand the strategies of Ga and In resistant strains to cope with these critical metals. This study aimed to explore the effect of these metals on two Ga/In resistant strains and to scrutinize the biological processes behind the oxidative stress in response to exposure to these critical metals. Two strains of Serratia fonticola, A3242 and B2A1Ga1, with high resistance to Ga and In, were submitted to metal stress and their protein profiles showed an overexpressed Superoxide Dismutase (SOD) in presence of In. Results of inhibitor-protein native gel incubations identified the overexpressed enzyme as a Fe-SOD. Both strains exhibited a huge increase of oxidative stress when exposed to indium, visible by an extreme high amount of reactive oxygen species (ROS) production. The toxicity induced by indium triggered biological mechanisms of stress control namely, the decrease in reduced glutathione/total glutathione levels and an increase in the SOD activity. The effect of gallium in cells was not so boisterous, visible only by the decrease of reduced glutathione levels. Analysis of the cellular metabolic viability revealed that each strain was affected differently by the critical metals, which could be related to the distinct metal uptakes. Strain A3242 accumulated more Ga and In in comparison to strain B2A1Ga1, and showed lower metabolic activity. Understanding the biological response of the two metal resistant strains of S. fonticola to stress induced by Ga and In will tackle the current gap of information related with bacteria-critical metals interactions.
format	article
author	Joana B. Caldeira Paula V. Morais Rita Branco
author_facet	Joana B. Caldeira Paula V. Morais Rita Branco
author_sort	Joana B. Caldeira
title	Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
title_short	Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
title_full	Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
title_fullStr	Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
title_full_unstemmed	Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium
title_sort	exploiting the biological response of two serratia fonticola strains to the critical metals, gallium and indium
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/941ca6f4d199473fbd71113cc0c72dbf
work_keys_str_mv	AT joanabcaldeira exploitingthebiologicalresponseoftwoserratiafonticolastrainstothecriticalmetalsgalliumandindium AT paulavmorais exploitingthebiologicalresponseoftwoserratiafonticolastrainstothecriticalmetalsgalliumandindium AT ritabranco exploitingthebiologicalresponseoftwoserratiafonticolastrainstothecriticalmetalsgalliumandindium
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Exploiting the biological response of two Serratia fonticola strains to the critical metals, gallium and indium

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