Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro

Background Dental caries is a multifactorial disease caused by pathogenic biofilm. In particular, Streptococcus mutans synthesizes biofilm exopolysaccharides, while Candida albicans is associated with the development of severe carious lesions. Aim This study aimed to prevent the formation of S. muta...

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Autores principales:	Guilherme Roncari Rocha, Kenneth R. Sims, Baixue Xiao, Marlise I. Klein, Danielle S.W. Benoit
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2022
Materias:	biofilm treatment dental caries streptococcus mutans candida albicans Infectious and parasitic diseases RC109-216 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9422be401bfb4e278e977dfa1a86e293
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spelling	oai:doaj.org-article:9422be401bfb4e278e977dfa1a86e2932021-11-26T11:19:49ZNanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro2000-229710.1080/20002297.2021.1997230https://doaj.org/article/9422be401bfb4e278e977dfa1a86e2932022-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20002297.2021.1997230https://doaj.org/toc/2000-2297Background Dental caries is a multifactorial disease caused by pathogenic biofilm. In particular, Streptococcus mutans synthesizes biofilm exopolysaccharides, while Candida albicans is associated with the development of severe carious lesions. Aim This study aimed to prevent the formation of S. mutans and C. albicans biofilms by exploiting pH-sensitive nanoparticle carriers (NPCs) with high affinity to exopolysaccharides to increase the substantivity of multi-targeted antibiofilm drugs introduced topically in vitro. Methods Dual-species biofilms were grown on saliva-coated hydroxyapatite discs with sucrose. Twice-daily, 1.5 min topical treatment regimens of unloaded and drug-loaded NPC were used. Drugs included combinations of two or three compounds with distinct, complementary antibiofilm targets: tt-farnesol (terpenoid; bacterial acid tolerance, fungal quorum sensing), myricetin (flavonoid; exopolysaccharides inhibitor), and 1771 (lipoteichoic acid inhibitor; bacterial adhesion and co-aggregation). Biofilms were evaluated for biomass, microbial population, and architecture. Results NPC delivering tt-farnesol and 1771 with or without myricetin completely prevented biofilm formation by impeding biomass accumulation, bacterial and fungal population growth, and exopolysaccharide matrix deposition (vs. control unloaded NPC). Both formulations hindered acid production, maintaining the pH of spent media above the threshold for enamel demineralization. However, treatments had no effect on pre-established dual-species biofilms. Conclusion Complementary antibiofilm drug-NPC treatments prevented biofilm formation by targeting critical virulence factors of acidogenicity and exopolysaccharides synthesis.Guilherme Roncari RochaKenneth R. SimsBaixue XiaoMarlise I. KleinDanielle S.W. BenoitTaylor & Francis Grouparticlebiofilmtreatmentdental cariesstreptococcus mutanscandida albicansInfectious and parasitic diseasesRC109-216MicrobiologyQR1-502ENJournal of Oral Microbiology, Vol 14, Iss 1 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm treatment dental caries streptococcus mutans candida albicans Infectious and parasitic diseases RC109-216 Microbiology QR1-502
spellingShingle	biofilm treatment dental caries streptococcus mutans candida albicans Infectious and parasitic diseases RC109-216 Microbiology QR1-502 Guilherme Roncari Rocha Kenneth R. Sims Baixue Xiao Marlise I. Klein Danielle S.W. Benoit Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
description	Background Dental caries is a multifactorial disease caused by pathogenic biofilm. In particular, Streptococcus mutans synthesizes biofilm exopolysaccharides, while Candida albicans is associated with the development of severe carious lesions. Aim This study aimed to prevent the formation of S. mutans and C. albicans biofilms by exploiting pH-sensitive nanoparticle carriers (NPCs) with high affinity to exopolysaccharides to increase the substantivity of multi-targeted antibiofilm drugs introduced topically in vitro. Methods Dual-species biofilms were grown on saliva-coated hydroxyapatite discs with sucrose. Twice-daily, 1.5 min topical treatment regimens of unloaded and drug-loaded NPC were used. Drugs included combinations of two or three compounds with distinct, complementary antibiofilm targets: tt-farnesol (terpenoid; bacterial acid tolerance, fungal quorum sensing), myricetin (flavonoid; exopolysaccharides inhibitor), and 1771 (lipoteichoic acid inhibitor; bacterial adhesion and co-aggregation). Biofilms were evaluated for biomass, microbial population, and architecture. Results NPC delivering tt-farnesol and 1771 with or without myricetin completely prevented biofilm formation by impeding biomass accumulation, bacterial and fungal population growth, and exopolysaccharide matrix deposition (vs. control unloaded NPC). Both formulations hindered acid production, maintaining the pH of spent media above the threshold for enamel demineralization. However, treatments had no effect on pre-established dual-species biofilms. Conclusion Complementary antibiofilm drug-NPC treatments prevented biofilm formation by targeting critical virulence factors of acidogenicity and exopolysaccharides synthesis.
format	article
author	Guilherme Roncari Rocha Kenneth R. Sims Baixue Xiao Marlise I. Klein Danielle S.W. Benoit
author_facet	Guilherme Roncari Rocha Kenneth R. Sims Baixue Xiao Marlise I. Klein Danielle S.W. Benoit
author_sort	Guilherme Roncari Rocha
title	Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
title_short	Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
title_full	Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
title_fullStr	Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
title_full_unstemmed	Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
title_sort	nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro
publisher	Taylor & Francis Group
publishDate	2022
url	https://doaj.org/article/9422be401bfb4e278e977dfa1a86e293
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Nanoparticle carrier co-delivery of complementary antibiofilm drugs abrogates dual species cariogenic biofilm formation in vitro

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