Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications

Metal–organic frameworks (MOFs) have been widely used as porous nanomaterials for different applications ranging from industrial to biomedicals. An unpredictable one-pot method is introduced to synthesize NH<sub>2</sub>-MIL-53 assisted by high-gravity in a greener media for the first tim...

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Autores principales:	Navid Rabiee, Mohammad Rabiee, Soheil Sojdeh, Yousef Fatahi, Rassoul Dinarvand, Moein Safarkhani, Sepideh Ahmadi, Hossein Daneshgar, Fatemeh Radmanesh, Saeid Maghsoudi, Mojtaba Bagherzadeh, Rajender S. Varma, Ebrahim Mostafavi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gene delivery CRISPR biosensor biomedicine MOF COVID-19 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/94244b7b1c6f44cbbc2f962ad9602574
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spelling	oai:doaj.org-article:94244b7b1c6f44cbbc2f962ad96025742021-11-25T16:54:13ZPorphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications10.3390/biom111117142218-273Xhttps://doaj.org/article/94244b7b1c6f44cbbc2f962ad96025742021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1714https://doaj.org/toc/2218-273XMetal–organic frameworks (MOFs) have been widely used as porous nanomaterials for different applications ranging from industrial to biomedicals. An unpredictable one-pot method is introduced to synthesize NH<sub>2</sub>-MIL-53 assisted by high-gravity in a greener media for the first time. Then, porphyrins were deployed to adorn the surface of MOF to increase the sensitivity of the prepared nanocomposite to the genetic materials and in-situ cellular protein structures. The hydrogen bond formation between genetic domains and the porphyrin’ nitrogen as well as the surface hydroxyl groups is equally probable and could be considered a milestone in chemical physics and physical chemistry for biomedical applications. In this context, the role of incorporating different forms of porphyrins, their relationship with the final surface morphology, and their drug/gene loading efficiency were investigated to provide a predictable pattern in regard to the previous works. The conceptual phenomenon was optimized to increase the interactions between the biomolecules and the substrate by reaching the limit of detection to 10 pM for the Anti-cas9 protein, 20 pM for the single-stranded DNA (ssDNA), below 10 pM for the single guide RNA (sgRNA) and also around 10 nM for recombinant SARS-CoV-2 spike antigen. Also, the MTT assay showed acceptable relative cell viability of more than 85% in most cases, even by increasing the dose of the prepared nanostructures.Navid RabieeMohammad RabieeSoheil SojdehYousef FatahiRassoul DinarvandMoein SafarkhaniSepideh AhmadiHossein DaneshgarFatemeh RadmaneshSaeid MaghsoudiMojtaba BagherzadehRajender S. VarmaEbrahim MostafaviMDPI AGarticlegene deliveryCRISPRbiosensorbiomedicineMOFCOVID-19MicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1714, p 1714 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gene delivery CRISPR biosensor biomedicine MOF COVID-19 Microbiology QR1-502
spellingShingle	gene delivery CRISPR biosensor biomedicine MOF COVID-19 Microbiology QR1-502 Navid Rabiee Mohammad Rabiee Soheil Sojdeh Yousef Fatahi Rassoul Dinarvand Moein Safarkhani Sepideh Ahmadi Hossein Daneshgar Fatemeh Radmanesh Saeid Maghsoudi Mojtaba Bagherzadeh Rajender S. Varma Ebrahim Mostafavi Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
description	Metal–organic frameworks (MOFs) have been widely used as porous nanomaterials for different applications ranging from industrial to biomedicals. An unpredictable one-pot method is introduced to synthesize NH<sub>2</sub>-MIL-53 assisted by high-gravity in a greener media for the first time. Then, porphyrins were deployed to adorn the surface of MOF to increase the sensitivity of the prepared nanocomposite to the genetic materials and in-situ cellular protein structures. The hydrogen bond formation between genetic domains and the porphyrin’ nitrogen as well as the surface hydroxyl groups is equally probable and could be considered a milestone in chemical physics and physical chemistry for biomedical applications. In this context, the role of incorporating different forms of porphyrins, their relationship with the final surface morphology, and their drug/gene loading efficiency were investigated to provide a predictable pattern in regard to the previous works. The conceptual phenomenon was optimized to increase the interactions between the biomolecules and the substrate by reaching the limit of detection to 10 pM for the Anti-cas9 protein, 20 pM for the single-stranded DNA (ssDNA), below 10 pM for the single guide RNA (sgRNA) and also around 10 nM for recombinant SARS-CoV-2 spike antigen. Also, the MTT assay showed acceptable relative cell viability of more than 85% in most cases, even by increasing the dose of the prepared nanostructures.
format	article
author	Navid Rabiee Mohammad Rabiee Soheil Sojdeh Yousef Fatahi Rassoul Dinarvand Moein Safarkhani Sepideh Ahmadi Hossein Daneshgar Fatemeh Radmanesh Saeid Maghsoudi Mojtaba Bagherzadeh Rajender S. Varma Ebrahim Mostafavi
author_facet	Navid Rabiee Mohammad Rabiee Soheil Sojdeh Yousef Fatahi Rassoul Dinarvand Moein Safarkhani Sepideh Ahmadi Hossein Daneshgar Fatemeh Radmanesh Saeid Maghsoudi Mojtaba Bagherzadeh Rajender S. Varma Ebrahim Mostafavi
author_sort	Navid Rabiee
title	Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
title_short	Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
title_full	Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
title_fullStr	Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
title_full_unstemmed	Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications
title_sort	porphyrin molecules decorated on metal-organic frameworks for multi-functional biomedical applications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/94244b7b1c6f44cbbc2f962ad9602574
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Porphyrin Molecules Decorated on Metal-Organic Frameworks for Multi-Functional Biomedical Applications

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