Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum

Abstract The E3 ubiquitin ligases have been known to intrigue many researchers to date, due to their heterogenicity and substrate mediation for ubiquitin transfer to the protein. HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ligases are spatially and temporally regulated for substrate specific...

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Autores principales:	Bhaskar Sharma, Harshita Saxena, Harshita Negi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9426a96df4f7442d8ec8da9365f24e93
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spelling	oai:doaj.org-article:9426a96df4f7442d8ec8da9365f24e932021-12-02T16:35:28ZGenome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum10.1038/s41598-021-95436-22045-2322https://doaj.org/article/9426a96df4f7442d8ec8da9365f24e932021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95436-2https://doaj.org/toc/2045-2322Abstract The E3 ubiquitin ligases have been known to intrigue many researchers to date, due to their heterogenicity and substrate mediation for ubiquitin transfer to the protein. HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ligases are spatially and temporally regulated for substrate specificity, E2 ubiquitin-conjugating enzyme interaction, and chain specificity during ubiquitylation. However, the role of the HECT E3 ubiquitin ligase in plant development and stress responses was rarely explored. We have conducted an in-silico genome-wide analysis to identify and predict the structural and functional aspects of HECT E3 ligase members in tomato. Fourteen members of HECT E3 ligases were identified and analyzed for the physicochemical parameters, phylogenetic relations, structural organizations, tissue-specific gene expression patterns, and protein interaction networks. Our comprehensive analysis revealed the HECT domain conservation throughout the gene family, close evolutionary relationship with different plant species, and active involvement of HECT E3 ubiquitin ligases in tomato plant development and stress responses. We speculate an indispensable biological significance of the HECT gene family through extensive participation in several plant cellular and molecular pathways.Bhaskar SharmaHarshita SaxenaHarshita NegiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bhaskar Sharma Harshita Saxena Harshita Negi Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
description	Abstract The E3 ubiquitin ligases have been known to intrigue many researchers to date, due to their heterogenicity and substrate mediation for ubiquitin transfer to the protein. HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ligases are spatially and temporally regulated for substrate specificity, E2 ubiquitin-conjugating enzyme interaction, and chain specificity during ubiquitylation. However, the role of the HECT E3 ubiquitin ligase in plant development and stress responses was rarely explored. We have conducted an in-silico genome-wide analysis to identify and predict the structural and functional aspects of HECT E3 ligase members in tomato. Fourteen members of HECT E3 ligases were identified and analyzed for the physicochemical parameters, phylogenetic relations, structural organizations, tissue-specific gene expression patterns, and protein interaction networks. Our comprehensive analysis revealed the HECT domain conservation throughout the gene family, close evolutionary relationship with different plant species, and active involvement of HECT E3 ubiquitin ligases in tomato plant development and stress responses. We speculate an indispensable biological significance of the HECT gene family through extensive participation in several plant cellular and molecular pathways.
format	article
author	Bhaskar Sharma Harshita Saxena Harshita Negi
author_facet	Bhaskar Sharma Harshita Saxena Harshita Negi
author_sort	Bhaskar Sharma
title	Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
title_short	Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
title_full	Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
title_fullStr	Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
title_full_unstemmed	Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum
title_sort	genome-wide analysis of hect e3 ubiquitin ligase gene family in solanum lycopersicum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9426a96df4f7442d8ec8da9365f24e93
work_keys_str_mv	AT bhaskarsharma genomewideanalysisofhecte3ubiquitinligasegenefamilyinsolanumlycopersicum AT harshitasaxena genomewideanalysisofhecte3ubiquitinligasegenefamilyinsolanumlycopersicum AT harshitanegi genomewideanalysisofhecte3ubiquitinligasegenefamilyinsolanumlycopersicum
_version_	1718383706664599552

Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum

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