A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells

Zhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, H...

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Autores principales:	Zhang ZQ, Yao WJ, Qiao LL, Yang X, Shi J, Zhao MX
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	quantum dots fluorescence probe lysosome-targetable cells imaging Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/942ca50ed3774677b4b1b38f015f397e
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spelling	oai:doaj.org-article:942ca50ed3774677b4b1b38f015f397e2021-12-02T09:15:11ZA Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells1178-2013https://doaj.org/article/942ca50ed3774677b4b1b38f015f397e2020-03-01T00:00:00Zhttps://www.dovepress.com/a-lysosome-targetable-fluorescence-probe-based-on-l-cysteine-polyamine-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle.Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1H NMR) spectroscopy, and 13C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV–Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM).Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV–Vis and fluorescence spectra demonstrated that the cysteine-polyamine-morpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells.Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.Keywords: quantum dots, fluorescence probe, lysosome-targetable, cells imagingZhang ZQYao WJQiao LLYang XShi JZhao MXDove Medical Pressarticlequantum dotsfluorescence probelysosome-targetablecells imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1611-1622 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantum dots fluorescence probe lysosome-targetable cells imaging Medicine (General) R5-920
spellingShingle	quantum dots fluorescence probe lysosome-targetable cells imaging Medicine (General) R5-920 Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
description	Zhi-Qiang Zhang, Wen-Jing Yao, Lu-Lu Qiao, Xiaojing Yang, Jiahua Shi, Mei-Xia Zhao Key Laboratory of Natural Medicine and Immune Engineering, Henan University, Kaifeng 475004, People’s Republic of ChinaCorrespondence: Mei-Xia ZhaoKey Laboratory of Natural Medicine and Immune Engineering, Henan University, Jinming Road, Kaifeng 475004, People’s Republic of ChinaEmail zhaomeixia2011@henu.edu.cnPurpose: Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle.Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1H NMR) spectroscopy, and 13C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV–Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM).Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV–Vis and fluorescence spectra demonstrated that the cysteine-polyamine-morpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells.Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.Keywords: quantum dots, fluorescence probe, lysosome-targetable, cells imaging
format	article
author	Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX
author_facet	Zhang ZQ Yao WJ Qiao LL Yang X Shi J Zhao MX
author_sort	Zhang ZQ
title	A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
title_short	A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
title_full	A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
title_fullStr	A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
title_full_unstemmed	A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells
title_sort	lysosome-targetable fluorescence probe based on l-cysteine-polyamine-morpholine-modified quantum dots for imaging in living cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/942ca50ed3774677b4b1b38f015f397e
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A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells

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